Application of porcine lipase secreted by pichia pastoris to improve fat digestion and growth performance of postweaning piglets

Fang Chueh Liu, Hsiao Ling Chen, Willie Lin, Y. U.Tang Tung, Cheng Wei Lai, A. L.I. Hsu, Chuan M.U. Chen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The aim of the study was to use Pichia pastoris to express a recombinant porcine lipase gene (pLip). The expression-secretion cassette was constructed using the P. pastoris GAPDH (glyceraldehyde-3-phosphate-dehydrogenase) promoter and an 89-residue prepro-α-factor secretion signal fused to the AOX1 terminator (the pGAPZαA vector). A total of 1,408 bp of pancreatic lipase cDNA was produced, which was located from the position of 4-nt upstream of ATG to 1408-nt inside the intact coding region of the pLip sequence. In an animal trial, three concentrations of recombinant lipase activity (0, 5,000 and 10,000 U/kg) were blended with the basal diet and fed to weaned piglets for six weeks. During the experimental period, the growth performance (bodyweight, feed intake, and feed efficiency) of the test groups was superior to that of the control group (p < 0.05). Furthermore, the group fed the diet blended with 10,000 U/kg of recombinant lipase showed significant (p < 0.05) increases in blood triglyceride (TG) concentration on the seventh day postweaning. These results suggested that the porcine lipase protein yielded by transformed yeast cells may improve fat digestibility and enhance the growth performance in postweaning piglets.

Original languageEnglish
Pages (from-to)3322-3329
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number6
DOIs
Publication statusPublished - Mar 24 2010
Externally publishedYes

Fingerprint

Pichia pastoris
Pichia
Lipase
piglets
Digestion
growth performance
Swine
digestion
Fats
swine
lipids
Growth
secretion
glyceraldehyde-3-phosphate dehydrogenase
Nutrition
diet
triacylglycerol lipase
feed conversion
feed intake
Genes

Keywords

  • Fat digestion
  • Pichia pastoris, animal feed
  • Porcine lipase
  • Postweaning piglet
  • Recombinant yeast

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Application of porcine lipase secreted by pichia pastoris to improve fat digestion and growth performance of postweaning piglets. / Liu, Fang Chueh; Chen, Hsiao Ling; Lin, Willie; Tung, Y. U.Tang; Lai, Cheng Wei; Hsu, A. L.I.; Chen, Chuan M.U.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 6, 24.03.2010, p. 3322-3329.

Research output: Contribution to journalArticle

Liu, Fang Chueh ; Chen, Hsiao Ling ; Lin, Willie ; Tung, Y. U.Tang ; Lai, Cheng Wei ; Hsu, A. L.I. ; Chen, Chuan M.U. / Application of porcine lipase secreted by pichia pastoris to improve fat digestion and growth performance of postweaning piglets. In: Journal of Agricultural and Food Chemistry. 2010 ; Vol. 58, No. 6. pp. 3322-3329.
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