Application of ECIS to assess FCCP-induced changes of MSC micromotion and wound healing migration

Sheng Po Chiu, Yu Wei Lee, Ling Yi Wu, Tse Hua Tung, Sofia Gomez, Chun Min Lo, Jia Yi Wang

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Electric cell-substrate impedance sensing (ECIS) is an emerging technique for sensitively monitoring morphological changes of adherent cells in tissue culture. In this study, human mesenchymal stem cells (hMSCs) were exposed to different concentrations of carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) for 20 h and their subsequent concentration-dependent responses in micromotion and wound healing migration were measured by ECIS. FCCP disrupts ATP synthesis and results in a decrease in cell migration rates. To detect the change of cell micromotion in response to FCCP challenge, time-series resistances of cell-covered electrodes were monitored and the values of variance were calculated to verify the difference. While Seahorse XF-24 extracellular flux analyzer can detect the effect of FCCP at 3 µM concentration, the variance calculation of the time-series resistances measured at 4 kHz can detect the effect of FCCP at concentrations as low as 1 µM. For wound healing migration, the recovery resistance curves were fitted by sigmoid curve and the hill slope showed a concentration-dependent decline from 0.3 µM to 3 µM, indicating a decrease in cell migration rate. Moreover, dose dependent incline of the inflection points from 0.3 µM to 3 µM FCCP implied the increase of the half time for wound recovery migration. Together, our results demonstrate that partial uncoupling of mitochondrial oxidative phosphorylation reduces micromotion and wound healing migration of hMSCs. The ECIS method used in this study offers a simple and sensitive approach to investigate stem cell migration and its regulation by mitochondrial dynamics.

Original languageEnglish
Article number3210
JournalSensors (Switzerland)
Volume19
Issue number14
DOIs
Publication statusPublished - Jul 2 2019

Keywords

  • Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP)
  • ECIS
  • Human mesenchymal stem cells (hMSCs)
  • Micromotion
  • Mitochondria
  • Wound healing migration

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Electrical and Electronic Engineering

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