Application of Adipose-Derived Stem Cells to Observe the Morphological Changes by Induction with Decellularized Corneal Extracellular Matrix and Specified Medium

Hsu Wei Fang, Li Ting Li, Shou Cheng Teng, Tsung Jen Wang

Research output: Contribution to journalArticle

Abstract

In recent years, many researches have focused on the behaviors of ADSCs induced with tissue-specific environment such as corneal extracellular matrix (ECM) due to their same embryonic origin from neural crest. In this study, human ADSCs were cultivated on bovine ECM (bECM) with different culture medium, including ADSC preserved medium (APM), supplemented hormonal epithelial medium (SHEM) and keratinocyte serum free medium (KSFM), compared to TCPS substrate. Cell morphology, proliferation and cytoskeleton markers were examined. The induction results implicated by morphology and immunostaining evidence showed that ADSCs exhibited spindle-shaped morphology on bECM cultured with APM and SHEM, while it displayed dendritic phenotype on bECM in KSFM. Moreover, the F-actin staining demonstrated a diffused expression in KSFM condition, which is different from a dense and thickened expression pattern in APM and SHEM when ADSCs were cultured on bECM. The ADSCs induced with serum-free medium and decellularized corneal ECM showed altered morphology and cytoskeleton structures different from serum-containing medium, which implied the differentiation potential of ADSCs and potential characteristics of corneal stromal cells.

Original languageEnglish
Pages (from-to)695-702
Number of pages8
JournalJournal of Medical and Biological Engineering
Volume37
Issue number5
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Serum-Free Culture Media
Stem cells
Military electronic countermeasures
Extracellular Matrix
Stem Cells
Keratinocytes
Cytoskeleton
Neural Crest
Stromal Cells
Culture Media
Actins
Cell Proliferation
Tissue
Staining and Labeling
Phenotype
Substrates
Serum
Research

Keywords

  • Adipose-derived stem cells
  • Cornea
  • Cytoskeleton

ASJC Scopus subject areas

  • Medicine(all)
  • Biomedical Engineering

Cite this

@article{ec9b62a83e1d42c28f2664ccdfbeb5c4,
title = "Application of Adipose-Derived Stem Cells to Observe the Morphological Changes by Induction with Decellularized Corneal Extracellular Matrix and Specified Medium",
abstract = "In recent years, many researches have focused on the behaviors of ADSCs induced with tissue-specific environment such as corneal extracellular matrix (ECM) due to their same embryonic origin from neural crest. In this study, human ADSCs were cultivated on bovine ECM (bECM) with different culture medium, including ADSC preserved medium (APM), supplemented hormonal epithelial medium (SHEM) and keratinocyte serum free medium (KSFM), compared to TCPS substrate. Cell morphology, proliferation and cytoskeleton markers were examined. The induction results implicated by morphology and immunostaining evidence showed that ADSCs exhibited spindle-shaped morphology on bECM cultured with APM and SHEM, while it displayed dendritic phenotype on bECM in KSFM. Moreover, the F-actin staining demonstrated a diffused expression in KSFM condition, which is different from a dense and thickened expression pattern in APM and SHEM when ADSCs were cultured on bECM. The ADSCs induced with serum-free medium and decellularized corneal ECM showed altered morphology and cytoskeleton structures different from serum-containing medium, which implied the differentiation potential of ADSCs and potential characteristics of corneal stromal cells.",
keywords = "Adipose-derived stem cells, Cornea, Cytoskeleton",
author = "Fang, {Hsu Wei} and Li, {Li Ting} and Teng, {Shou Cheng} and Wang, {Tsung Jen}",
year = "2017",
month = "10",
day = "1",
doi = "10.1007/s40846-017-0268-7",
language = "English",
volume = "37",
pages = "695--702",
journal = "Journal of Medical and Biological Engineering",
issn = "1609-0985",
publisher = "中華民國生物醫學工程學會",
number = "5",

}

TY - JOUR

T1 - Application of Adipose-Derived Stem Cells to Observe the Morphological Changes by Induction with Decellularized Corneal Extracellular Matrix and Specified Medium

AU - Fang, Hsu Wei

AU - Li, Li Ting

AU - Teng, Shou Cheng

AU - Wang, Tsung Jen

PY - 2017/10/1

Y1 - 2017/10/1

N2 - In recent years, many researches have focused on the behaviors of ADSCs induced with tissue-specific environment such as corneal extracellular matrix (ECM) due to their same embryonic origin from neural crest. In this study, human ADSCs were cultivated on bovine ECM (bECM) with different culture medium, including ADSC preserved medium (APM), supplemented hormonal epithelial medium (SHEM) and keratinocyte serum free medium (KSFM), compared to TCPS substrate. Cell morphology, proliferation and cytoskeleton markers were examined. The induction results implicated by morphology and immunostaining evidence showed that ADSCs exhibited spindle-shaped morphology on bECM cultured with APM and SHEM, while it displayed dendritic phenotype on bECM in KSFM. Moreover, the F-actin staining demonstrated a diffused expression in KSFM condition, which is different from a dense and thickened expression pattern in APM and SHEM when ADSCs were cultured on bECM. The ADSCs induced with serum-free medium and decellularized corneal ECM showed altered morphology and cytoskeleton structures different from serum-containing medium, which implied the differentiation potential of ADSCs and potential characteristics of corneal stromal cells.

AB - In recent years, many researches have focused on the behaviors of ADSCs induced with tissue-specific environment such as corneal extracellular matrix (ECM) due to their same embryonic origin from neural crest. In this study, human ADSCs were cultivated on bovine ECM (bECM) with different culture medium, including ADSC preserved medium (APM), supplemented hormonal epithelial medium (SHEM) and keratinocyte serum free medium (KSFM), compared to TCPS substrate. Cell morphology, proliferation and cytoskeleton markers were examined. The induction results implicated by morphology and immunostaining evidence showed that ADSCs exhibited spindle-shaped morphology on bECM cultured with APM and SHEM, while it displayed dendritic phenotype on bECM in KSFM. Moreover, the F-actin staining demonstrated a diffused expression in KSFM condition, which is different from a dense and thickened expression pattern in APM and SHEM when ADSCs were cultured on bECM. The ADSCs induced with serum-free medium and decellularized corneal ECM showed altered morphology and cytoskeleton structures different from serum-containing medium, which implied the differentiation potential of ADSCs and potential characteristics of corneal stromal cells.

KW - Adipose-derived stem cells

KW - Cornea

KW - Cytoskeleton

UR - http://www.scopus.com/inward/record.url?scp=85029852178&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029852178&partnerID=8YFLogxK

U2 - 10.1007/s40846-017-0268-7

DO - 10.1007/s40846-017-0268-7

M3 - Article

VL - 37

SP - 695

EP - 702

JO - Journal of Medical and Biological Engineering

JF - Journal of Medical and Biological Engineering

SN - 1609-0985

IS - 5

ER -