Apoptotic toxicity of destruxin B in human non-Hodgkin lymphoma cells

Pin Zhir Chao, Yi Ping Chin, I. Uen Hsu, Cheng-Ming Liu, Ya Chi Yu, Ting-Kai Leung, Yuarn Jang Lee, Chien Ho Chen, Yung Feng Lin

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4 Citations (Scopus)

Abstract

Destruxins are fungal toxins used as insecticides. Recent reports demonstrated the potential anti-cancer activities of destruxin B (DB). This study is to discover the effects of DB in lymphoma. Flow cytometry and Western blotting were used to analyze apoptosis and protein expression, respectively, in Toledo human non-Hodgkin lymphoma cells in response to DB. Administration of DB, induced apoptosis via death receptor pathway activating Fas associated death domain (FADD), caspase 8 and caspase 3, and suppressed the cell growth. In addition, DB alterated mitochondrial membrane potential by increasing the expressions of tBid and Bax, but decreasing the levels of Bcl-2, resulting in the release of apoptosis-inducing factor (AIF). In conclusion, apoptosis of human non-Hodgkin lymphoma cells in response to DB is induced through the death receptor pathway and involves an alteration of the mitochondrial membrane potential. These findings may aid the development of novel treatment of non-Hodgkin lymphoma.

Original languageEnglish
Pages (from-to)1870-1876
Number of pages7
JournalToxicology in Vitro
Volume27
Issue number6
DOIs
Publication statusPublished - Sep 2013

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Keywords

  • Apoptosis
  • Death receptor pathway
  • Destruxin B
  • Mitochondrial membrane potential
  • Non-Hodgkin lymphoma

ASJC Scopus subject areas

  • Toxicology

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