Apoptosis-related phenotype of ejaculated spermatozoa in patients with varicocele

Gwo Jang Wu, Fung W. Chang, Shang S. Lee, Ya Yuan Cheng, Chi Huang Chen, I. Ching Chen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objective: To determine the integrity of the sperm plasma membrane, mitochondria, and DNA, which are essential for accurate transmission of genetic material to offspring, and to quantify possible apoptosis and investigate any relationship between these parameters in ejaculated sperm from men with or without varicoceles. Design: Retrospective study. Setting: University teaching hospital. Patient(s): Twenty-five patients with varicocele and 10 normal, fertile controls. Intervention(s): Apoptosis-related phenotype activations including the plasma membrane translocation of phosphatidylserine, mitochondrial dysfunction, and nuclear DNA damage, were assessed by using the annexin-V/propidium iodide double staining assay, 3,3'-dihexloxacarbocyanine iodide staining assay, and single-cell gel electrophoresis assay (comet assay). Main Outcome Measure(s): Apoptosis-related phenotype. Result(s): Patients with varicocele had statistically significantly more annexin V live sperm cells and nuclear DNA fragmentation than did the control men. In contrast, their numbers of 3,3′-dihexloxacarbocyanine iodide live cells were statistically significantly less than those in control men. Conclusion(s): The increased externalization of phosphatidylserine, mitochondrial dysfunction, and nuclear DNA damage occurred in the sperm of men with varicoceles, suggesting that certain apoptotic mechanisms may relate to the condition of varicocele, originating in the mitochondria of spermatocytes and then functioning within the nucleus of the cell.

Original languageEnglish
Pages (from-to)831-837
Number of pages7
JournalFertility and Sterility
Volume91
Issue number3
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

Fingerprint

Varicocele
Spermatozoa
Apoptosis
Phenotype
Comet Assay
Annexin A5
Phosphatidylserines
Iodides
DNA Damage
Mitochondria
Cell Membrane
Staining and Labeling
Spermatocytes
Propidium
DNA Fragmentation
Cell Nucleus
Teaching Hospitals
Retrospective Studies
Outcome Assessment (Health Care)
DNA

Keywords

  • Apoptosis
  • DNA fragmentation
  • phosphatidylserine
  • sperm
  • varicocele

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Apoptosis-related phenotype of ejaculated spermatozoa in patients with varicocele. / Wu, Gwo Jang; Chang, Fung W.; Lee, Shang S.; Cheng, Ya Yuan; Chen, Chi Huang; Chen, I. Ching.

In: Fertility and Sterility, Vol. 91, No. 3, 03.2009, p. 831-837.

Research output: Contribution to journalArticle

Wu, Gwo Jang ; Chang, Fung W. ; Lee, Shang S. ; Cheng, Ya Yuan ; Chen, Chi Huang ; Chen, I. Ching. / Apoptosis-related phenotype of ejaculated spermatozoa in patients with varicocele. In: Fertility and Sterility. 2009 ; Vol. 91, No. 3. pp. 831-837.
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