Apoptosis of human retina and retinal pigment cells induced by human cytomegalovirus infection

Shih Hwa Chiou, Jorn Hon Liu, Steve S L Chen, Wu Tse Liu, Jung Chun Lin, Wing Wai Wong, Wen Ser Tseng, Ching Kuang Chou, Cheng Yi Liu, Larry L T Ho, Wen-Ming Hsu

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15 Citations (Scopus)

Abstract

Human cytomegalovirus (HCMV) retinitis is the most common ocular opportunistic infection in immunocompromised patients and AIDS. It often leads to blindness if left untreated. The question as to how HCMV infection causes retinal pathogenesis and visual destruction in AIDS patients remains unresolved. To answer the question, by using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay, we detected the significant signals of apoptotic cells at the same sites in the HCMV-infected retina of AIDS patients as compared to AIDS patients without HCMV retinitis. In vitro study also revealed apoptosis induced by HCMV infection in human retinal pigment epithelium cells, mediated by activation of caspase 3 and poly(ADP-ribose) polymerase pathway. These results strongly suggest the fundamental role of HCMV-induced apoptosis in mediating cell death in infected human retina and retinal pigment epithelium cells to make severe visual impairment.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalOphthalmic Research
Volume34
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Caspase 3
  • Human cytomegalovirus
  • Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL)

ASJC Scopus subject areas

  • Ophthalmology

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  • Cite this

    Chiou, S. H., Liu, J. H., Chen, S. S. L., Liu, W. T., Lin, J. C., Wong, W. W., Tseng, W. S., Chou, C. K., Liu, C. Y., Ho, L. L. T., & Hsu, W-M. (2002). Apoptosis of human retina and retinal pigment cells induced by human cytomegalovirus infection. Ophthalmic Research, 34(2), 77-82. https://doi.org/10.1159/000048332