Apelin regulates the electrophysiological characteristics of atrial myocytes

Chen Chuan Cheng, Punate Weerateerangkul, Yen Yu Lu, Yao Chang Chen, Yung Kuo Lin, Shih Ann Chen, Yi Jen Chen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Backgroud: Apelin, a potential agent for treating heart failure, has various ionic effects on ventricular myocytes. However, the effects of apelin on the atrium are not clear. The purpose of this study was to investigate the acute effects of apelin on the electrophysiological characteristics of atrial myocytes. Method: Whole-cell patch-clamp techniques were used to investigate the action potential (AP) and ionic currents in isolated rabbit left atrial (LA) myocytes before and after the administration of apelin. Result: Apelin reduced LA AP duration measured at 90%, 50% and 20% repolarization of the amplitude by 11±3%, 24±5%, 30±7% at 1nM (n=11), and by 14±4%, 36±6% and 45±5% at 10nM (n=11), but not at 0·1nM. Apeline (0·1, 1, 10nM) did not change the amplitude, or resting membrane potential in LA myocytes. Apelin (1nM) increased sodium currents, ultra-rapid potassium currents and the reverse mode of sodium-calcium exchanger currents, but decreased late sodium currents and L-type calcium currents and did not change transient outward currents or inward rectifier potassium currents in LA myocytes. Conclusions: Apelin significantly changed the atrial electrophysiology with a shortening of AP duration, which may be caused by its effects on multiple ionic currents.

Original languageEnglish
Pages (from-to)34-40
Number of pages7
JournalEuropean Journal of Clinical Investigation
Volume43
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Muscle Cells
Potassium
Sodium
Sodium-Calcium Exchanger
Electrophysiology
Clamping devices
Action Potentials
Calcium
Membranes
Patch-Clamp Techniques
Membrane Potentials
Heart Failure
Rabbits

Keywords

  • Apelin
  • Atrium
  • Cardiac electrophysiology
  • Isolated cardiomyocytes

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Biochemistry
  • Biochemistry

Cite this

Apelin regulates the electrophysiological characteristics of atrial myocytes. / Cheng, Chen Chuan; Weerateerangkul, Punate; Lu, Yen Yu; Chen, Yao Chang; Lin, Yung Kuo; Chen, Shih Ann; Chen, Yi Jen.

In: European Journal of Clinical Investigation, Vol. 43, No. 1, 01.2013, p. 34-40.

Research output: Contribution to journalArticle

Cheng, Chen Chuan ; Weerateerangkul, Punate ; Lu, Yen Yu ; Chen, Yao Chang ; Lin, Yung Kuo ; Chen, Shih Ann ; Chen, Yi Jen. / Apelin regulates the electrophysiological characteristics of atrial myocytes. In: European Journal of Clinical Investigation. 2013 ; Vol. 43, No. 1. pp. 34-40.
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AB - Backgroud: Apelin, a potential agent for treating heart failure, has various ionic effects on ventricular myocytes. However, the effects of apelin on the atrium are not clear. The purpose of this study was to investigate the acute effects of apelin on the electrophysiological characteristics of atrial myocytes. Method: Whole-cell patch-clamp techniques were used to investigate the action potential (AP) and ionic currents in isolated rabbit left atrial (LA) myocytes before and after the administration of apelin. Result: Apelin reduced LA AP duration measured at 90%, 50% and 20% repolarization of the amplitude by 11±3%, 24±5%, 30±7% at 1nM (n=11), and by 14±4%, 36±6% and 45±5% at 10nM (n=11), but not at 0·1nM. Apeline (0·1, 1, 10nM) did not change the amplitude, or resting membrane potential in LA myocytes. Apelin (1nM) increased sodium currents, ultra-rapid potassium currents and the reverse mode of sodium-calcium exchanger currents, but decreased late sodium currents and L-type calcium currents and did not change transient outward currents or inward rectifier potassium currents in LA myocytes. Conclusions: Apelin significantly changed the atrial electrophysiology with a shortening of AP duration, which may be caused by its effects on multiple ionic currents.

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