Apamin modulates electrophysiological characteristics of the pulmonary vein and the Sinoatrial Node

Wei Ta Chen; Yao Chang Chen; Yen Yu Lu; Yu Hsun Kao; Jen Hung Huang; Yung Kuo Lin; Shih Ann Chen; Yi Jen Chen

doi:10.1111/eci.12125

Apamin modulates electrophysiological characteristics of the pulmonary vein and the Sinoatrial Node

Wei Ta Chen, Yao Chang Chen, Yen Yu Lu, Yu Hsun Kao, Jen Hung Huang, Yung Kuo Lin, Shih Ann Chen, Yi Jen Chen

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Background: Small-conductance calcium-activated potassium (SK) channels play an important role in atrial electrophysiology. Blocking SK channels prolongs action potential (AP) duration and attenuate electrical remodelling. The effects of SK blocking on the pulmonary vein (PV) and the sinoatrial node (SAN) remain unclear. Materials and methods: Conventional microelectrodes were used to record the AP in SANs and in isolated rabbit PVs with and without denudation before and after treatment with apamin (1, 10 nM). Using the whole-cell patch-clamp technique, the SK current was investigated in isolated single PV and SAN myocytes. Results: In nondenudated PVs (n = 6), apamin (1 and 10 nM) increased PV spontaneous activity (from 1·8 ± 0·2 Hz to 2·4 ± 0·2 Hz and 2·3 ± 0·2 Hz, P

Original language	English
Pages (from-to)	957-963
Number of pages	7
Journal	European Journal of Clinical Investigation
Volume	43
Issue number	9
DOIs	https://doi.org/10.1111/eci.12125
Publication status	Published - Sep 2013

Keywords

Apamin
Atrial fibrillation
Pulmonary vein
Sinoatrial node
Small-conductance calcium-activated potassium current

ASJC Scopus subject areas

Medicine(all)
Clinical Biochemistry
Biochemistry

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Apamin modulates electrophysiological characteristics of the pulmonary vein and the Sinoatrial Node. / Chen, Wei Ta; Chen, Yao Chang; Lu, Yen Yu; Kao, Yu Hsun ; Huang, Jen Hung ; Lin, Yung Kuo; Chen, Shih Ann; Chen, Yi Jen.

In: European Journal of Clinical Investigation, Vol. 43, No. 9, 09.2013, p. 957-963.

Research output: Contribution to journal › Article › peer-review

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abstract = "Background: Small-conductance calcium-activated potassium (SK) channels play an important role in atrial electrophysiology. Blocking SK channels prolongs action potential (AP) duration and attenuate electrical remodelling. The effects of SK blocking on the pulmonary vein (PV) and the sinoatrial node (SAN) remain unclear. Materials and methods: Conventional microelectrodes were used to record the AP in SANs and in isolated rabbit PVs with and without denudation before and after treatment with apamin (1, 10 nM). Using the whole-cell patch-clamp technique, the SK current was investigated in isolated single PV and SAN myocytes. Results: In nondenudated PVs (n = 6), apamin (1 and 10 nM) increased PV spontaneous activity (from 1·8 ± 0·2 Hz to 2·4 ± 0·2 Hz and 2·3 ± 0·2 Hz, P ",

keywords = "Apamin, Atrial fibrillation, Pulmonary vein, Sinoatrial node, Small-conductance calcium-activated potassium current",

author = "Chen, {Wei Ta} and Chen, {Yao Chang} and Lu, {Yen Yu} and Kao, {Yu Hsun} and Huang, {Jen Hung} and Lin, {Yung Kuo} and Chen, {Shih Ann} and Chen, {Yi Jen}",

year = "2013",

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AU - Chen, Wei Ta

AU - Chen, Yao Chang

AU - Lu, Yen Yu

AU - Kao, Yu Hsun

AU - Huang, Jen Hung

AU - Lin, Yung Kuo

AU - Chen, Shih Ann

AU - Chen, Yi Jen

PY - 2013/9

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N2 - Background: Small-conductance calcium-activated potassium (SK) channels play an important role in atrial electrophysiology. Blocking SK channels prolongs action potential (AP) duration and attenuate electrical remodelling. The effects of SK blocking on the pulmonary vein (PV) and the sinoatrial node (SAN) remain unclear. Materials and methods: Conventional microelectrodes were used to record the AP in SANs and in isolated rabbit PVs with and without denudation before and after treatment with apamin (1, 10 nM). Using the whole-cell patch-clamp technique, the SK current was investigated in isolated single PV and SAN myocytes. Results: In nondenudated PVs (n = 6), apamin (1 and 10 nM) increased PV spontaneous activity (from 1·8 ± 0·2 Hz to 2·4 ± 0·2 Hz and 2·3 ± 0·2 Hz, P

AB - Background: Small-conductance calcium-activated potassium (SK) channels play an important role in atrial electrophysiology. Blocking SK channels prolongs action potential (AP) duration and attenuate electrical remodelling. The effects of SK blocking on the pulmonary vein (PV) and the sinoatrial node (SAN) remain unclear. Materials and methods: Conventional microelectrodes were used to record the AP in SANs and in isolated rabbit PVs with and without denudation before and after treatment with apamin (1, 10 nM). Using the whole-cell patch-clamp technique, the SK current was investigated in isolated single PV and SAN myocytes. Results: In nondenudated PVs (n = 6), apamin (1 and 10 nM) increased PV spontaneous activity (from 1·8 ± 0·2 Hz to 2·4 ± 0·2 Hz and 2·3 ± 0·2 Hz, P

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KW - Atrial fibrillation

KW - Pulmonary vein

KW - Sinoatrial node

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Apamin modulates electrophysiological characteristics of the pulmonary vein and the Sinoatrial Node

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