Abstract
Background: Small-conductance calcium-activated potassium (SK) channels play an important role in atrial electrophysiology. Blocking SK channels prolongs action potential (AP) duration and attenuate electrical remodelling. The effects of SK blocking on the pulmonary vein (PV) and the sinoatrial node (SAN) remain unclear. Materials and methods: Conventional microelectrodes were used to record the AP in SANs and in isolated rabbit PVs with and without denudation before and after treatment with apamin (1, 10 nM). Using the whole-cell patch-clamp technique, the SK current was investigated in isolated single PV and SAN myocytes. Results: In nondenudated PVs (n = 6), apamin (1 and 10 nM) increased PV spontaneous activity (from 1·8 ± 0·2 Hz to 2·4 ± 0·2 Hz and 2·3 ± 0·2 Hz, P
| Original language | English |
|---|---|
| Pages (from-to) | 957-963 |
| Number of pages | 7 |
| Journal | European Journal of Clinical Investigation |
| Volume | 43 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 2013 |
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Keywords
- Apamin
- Atrial fibrillation
- Pulmonary vein
- Sinoatrial node
- Small-conductance calcium-activated potassium current
ASJC Scopus subject areas
- Medicine(all)
- Clinical Biochemistry
- Biochemistry
Cite this
Apamin modulates electrophysiological characteristics of the pulmonary vein and the Sinoatrial Node. / Chen, Wei Ta; Chen, Yao Chang; Lu, Yen Yu; Kao, Yu Hsun; Huang, Jen Hung; Lin, Yung Kuo; Chen, Shih Ann; Chen, Yi Jen.
In: European Journal of Clinical Investigation, Vol. 43, No. 9, 09.2013, p. 957-963.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apamin modulates electrophysiological characteristics of the pulmonary vein and the Sinoatrial Node
AU - Chen, Wei Ta
AU - Chen, Yao Chang
AU - Lu, Yen Yu
AU - Kao, Yu Hsun
AU - Huang, Jen Hung
AU - Lin, Yung Kuo
AU - Chen, Shih Ann
AU - Chen, Yi Jen
PY - 2013/9
Y1 - 2013/9
N2 - Background: Small-conductance calcium-activated potassium (SK) channels play an important role in atrial electrophysiology. Blocking SK channels prolongs action potential (AP) duration and attenuate electrical remodelling. The effects of SK blocking on the pulmonary vein (PV) and the sinoatrial node (SAN) remain unclear. Materials and methods: Conventional microelectrodes were used to record the AP in SANs and in isolated rabbit PVs with and without denudation before and after treatment with apamin (1, 10 nM). Using the whole-cell patch-clamp technique, the SK current was investigated in isolated single PV and SAN myocytes. Results: In nondenudated PVs (n = 6), apamin (1 and 10 nM) increased PV spontaneous activity (from 1·8 ± 0·2 Hz to 2·4 ± 0·2 Hz and 2·3 ± 0·2 Hz, P
AB - Background: Small-conductance calcium-activated potassium (SK) channels play an important role in atrial electrophysiology. Blocking SK channels prolongs action potential (AP) duration and attenuate electrical remodelling. The effects of SK blocking on the pulmonary vein (PV) and the sinoatrial node (SAN) remain unclear. Materials and methods: Conventional microelectrodes were used to record the AP in SANs and in isolated rabbit PVs with and without denudation before and after treatment with apamin (1, 10 nM). Using the whole-cell patch-clamp technique, the SK current was investigated in isolated single PV and SAN myocytes. Results: In nondenudated PVs (n = 6), apamin (1 and 10 nM) increased PV spontaneous activity (from 1·8 ± 0·2 Hz to 2·4 ± 0·2 Hz and 2·3 ± 0·2 Hz, P
KW - Apamin
KW - Atrial fibrillation
KW - Pulmonary vein
KW - Sinoatrial node
KW - Small-conductance calcium-activated potassium current
UR - http://www.scopus.com/inward/record.url?scp=84881665628&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881665628&partnerID=8YFLogxK
U2 - 10.1111/eci.12125
DO - 10.1111/eci.12125
M3 - Article
C2 - 23834267
AN - SCOPUS:84881665628
VL - 43
SP - 957
EP - 963
JO - European Journal of Clinical Investigation
JF - European Journal of Clinical Investigation
SN - 0014-2972
IS - 9
ER -