Apamin modulates electrophysiological characteristics of the pulmonary vein and the Sinoatrial Node

Wei Ta Chen, Yao Chang Chen, Yen Yu Lu, Yu Hsun Kao, Jen Hung Huang, Yung Kuo Lin, Shih Ann Chen, Yi Jen Chen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Small-conductance calcium-activated potassium (SK) channels play an important role in atrial electrophysiology. Blocking SK channels prolongs action potential (AP) duration and attenuate electrical remodelling. The effects of SK blocking on the pulmonary vein (PV) and the sinoatrial node (SAN) remain unclear. Materials and methods: Conventional microelectrodes were used to record the AP in SANs and in isolated rabbit PVs with and without denudation before and after treatment with apamin (1, 10 nM). Using the whole-cell patch-clamp technique, the SK current was investigated in isolated single PV and SAN myocytes. Results: In nondenudated PVs (n = 6), apamin (1 and 10 nM) increased PV spontaneous activity (from 1·8 ± 0·2 Hz to 2·4 ± 0·2 Hz and 2·3 ± 0·2 Hz, P 

Original languageEnglish
Pages (from-to)957-963
Number of pages7
JournalEuropean Journal of Clinical Investigation
Volume43
Issue number9
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Apamin
Sinoatrial Node
Pulmonary Veins
Small-Conductance Calcium-Activated Potassium Channels
Electrophysiology
Action Potentials
Microelectrodes
Clamping devices
Atrial Remodeling
Patch-Clamp Techniques
Calcium
Muscle Cells
Rabbits

Keywords

  • Apamin
  • Atrial fibrillation
  • Pulmonary vein
  • Sinoatrial node
  • Small-conductance calcium-activated potassium current

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Biochemistry
  • Biochemistry

Cite this

Apamin modulates electrophysiological characteristics of the pulmonary vein and the Sinoatrial Node. / Chen, Wei Ta; Chen, Yao Chang; Lu, Yen Yu; Kao, Yu Hsun; Huang, Jen Hung; Lin, Yung Kuo; Chen, Shih Ann; Chen, Yi Jen.

In: European Journal of Clinical Investigation, Vol. 43, No. 9, 09.2013, p. 957-963.

Research output: Contribution to journalArticle

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AU - Chen, Wei Ta

AU - Chen, Yao Chang

AU - Lu, Yen Yu

AU - Kao, Yu Hsun

AU - Huang, Jen Hung

AU - Lin, Yung Kuo

AU - Chen, Shih Ann

AU - Chen, Yi Jen

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N2 - Background: Small-conductance calcium-activated potassium (SK) channels play an important role in atrial electrophysiology. Blocking SK channels prolongs action potential (AP) duration and attenuate electrical remodelling. The effects of SK blocking on the pulmonary vein (PV) and the sinoatrial node (SAN) remain unclear. Materials and methods: Conventional microelectrodes were used to record the AP in SANs and in isolated rabbit PVs with and without denudation before and after treatment with apamin (1, 10 nM). Using the whole-cell patch-clamp technique, the SK current was investigated in isolated single PV and SAN myocytes. Results: In nondenudated PVs (n = 6), apamin (1 and 10 nM) increased PV spontaneous activity (from 1·8 ± 0·2 Hz to 2·4 ± 0·2 Hz and 2·3 ± 0·2 Hz, P 

AB - Background: Small-conductance calcium-activated potassium (SK) channels play an important role in atrial electrophysiology. Blocking SK channels prolongs action potential (AP) duration and attenuate electrical remodelling. The effects of SK blocking on the pulmonary vein (PV) and the sinoatrial node (SAN) remain unclear. Materials and methods: Conventional microelectrodes were used to record the AP in SANs and in isolated rabbit PVs with and without denudation before and after treatment with apamin (1, 10 nM). Using the whole-cell patch-clamp technique, the SK current was investigated in isolated single PV and SAN myocytes. Results: In nondenudated PVs (n = 6), apamin (1 and 10 nM) increased PV spontaneous activity (from 1·8 ± 0·2 Hz to 2·4 ± 0·2 Hz and 2·3 ± 0·2 Hz, P 

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