Abstract

Antroquinonol (ANQ) is a ubiquinone derivative from the unique mushroom Antrodia camphorata, which exhibits broad-spectrum bioactivities. The effects of ANQ on cancer stem cell-like properties in colon cancer, however, remain unclear. In this study, we found that ANQ inhibited growth of colon cancer cells. The 50% growth inhibitions (GI50) of ANQ on HCT15 and LoVo were 34.8 ± 0.07 and 17.9 ± 0.07 μM. Moreover, ANQ exhibited inhibitory activities toward migration/ invasion and tumorsphere formation of colon cancer cells. Mechanistically, ANQ inhibited pluripotent and cancer stem cellrelated genes and down-regulated β-catenin/T-cell factor (TCF) signaling. Moreover, activation of the phosphatidylinositol-3- kinase (PI3K)/AKT/β-catenin signaling axis was identified to be crucial for regulating the expressions of pluripotent genes, whereas suppression of PI3K/AKT by ANQ inhibited expressions of β-catenin and downstream targets. Molecular docking identified the potential interaction of ANQ with PI3K. Our data show for the first time that the bioactive component of A. camphorata, ANQ, suppresses stem cell-like properties via targeting PI3K/AKT/β-catenin signaling. ANQ could be a promising cancer prevention agent for colon cancer.

Original languageEnglish
Pages (from-to)51-59
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

Antrodia
Taiwanofungus camphoratus
Ubiquinone
Neoplastic Stem Cells
ubiquinones
Agaricales
phosphatidylinositol 3-kinase
Stem cells
colorectal neoplasms
mushrooms
Colonic Neoplasms
stem cells
chemical derivatives
Derivatives
Phosphatidylinositol 3-Kinase
Catenins
neoplasms
growth retardation
genes
T-lymphocytes

Keywords

  • Antrodia camphorata
  • Antroquinonol
  • Cancer prevention
  • Colon cancer stem cell
  • β-catenin

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

@article{74290181311b491d84c85fe6628e0bb7,
title = "Antroquinonol, a ubiquinone derivative from the mushroom antrodia camphorata, inhibits colon cancer stem cell-like properties: Insights into the molecular mechanism & inhibitory targets",
abstract = "Antroquinonol (ANQ) is a ubiquinone derivative from the unique mushroom Antrodia camphorata, which exhibits broad-spectrum bioactivities. The effects of ANQ on cancer stem cell-like properties in colon cancer, however, remain unclear. In this study, we found that ANQ inhibited growth of colon cancer cells. The 50{\%} growth inhibitions (GI50) of ANQ on HCT15 and LoVo were 34.8 ± 0.07 and 17.9 ± 0.07 μM. Moreover, ANQ exhibited inhibitory activities toward migration/ invasion and tumorsphere formation of colon cancer cells. Mechanistically, ANQ inhibited pluripotent and cancer stem cellrelated genes and down-regulated β-catenin/T-cell factor (TCF) signaling. Moreover, activation of the phosphatidylinositol-3- kinase (PI3K)/AKT/β-catenin signaling axis was identified to be crucial for regulating the expressions of pluripotent genes, whereas suppression of PI3K/AKT by ANQ inhibited expressions of β-catenin and downstream targets. Molecular docking identified the potential interaction of ANQ with PI3K. Our data show for the first time that the bioactive component of A. camphorata, ANQ, suppresses stem cell-like properties via targeting PI3K/AKT/β-catenin signaling. ANQ could be a promising cancer prevention agent for colon cancer.",
keywords = "Antrodia camphorata, Antroquinonol, Cancer prevention, Colon cancer stem cell, β-catenin",
author = "Lin, {Hsien Chun} and Lin, {Mei Hsiang} and Liao, {Jiahn Haur} and Wu, {Tzu Hua} and Lee, {Tzong Huei} and Mi, {Fwu Long} and Wu, {Chi Hao} and Chen, {Ku Chung} and Cheng, {Chia Hsiung} and Lin, {Cheng Wei}",
year = "2017",
doi = "10.1021/acs.jafc.6b04101",
language = "English",
volume = "65",
pages = "51--59",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "1",

}

TY - JOUR

T1 - Antroquinonol, a ubiquinone derivative from the mushroom antrodia camphorata, inhibits colon cancer stem cell-like properties

T2 - Insights into the molecular mechanism & inhibitory targets

AU - Lin, Hsien Chun

AU - Lin, Mei Hsiang

AU - Liao, Jiahn Haur

AU - Wu, Tzu Hua

AU - Lee, Tzong Huei

AU - Mi, Fwu Long

AU - Wu, Chi Hao

AU - Chen, Ku Chung

AU - Cheng, Chia Hsiung

AU - Lin, Cheng Wei

PY - 2017

Y1 - 2017

N2 - Antroquinonol (ANQ) is a ubiquinone derivative from the unique mushroom Antrodia camphorata, which exhibits broad-spectrum bioactivities. The effects of ANQ on cancer stem cell-like properties in colon cancer, however, remain unclear. In this study, we found that ANQ inhibited growth of colon cancer cells. The 50% growth inhibitions (GI50) of ANQ on HCT15 and LoVo were 34.8 ± 0.07 and 17.9 ± 0.07 μM. Moreover, ANQ exhibited inhibitory activities toward migration/ invasion and tumorsphere formation of colon cancer cells. Mechanistically, ANQ inhibited pluripotent and cancer stem cellrelated genes and down-regulated β-catenin/T-cell factor (TCF) signaling. Moreover, activation of the phosphatidylinositol-3- kinase (PI3K)/AKT/β-catenin signaling axis was identified to be crucial for regulating the expressions of pluripotent genes, whereas suppression of PI3K/AKT by ANQ inhibited expressions of β-catenin and downstream targets. Molecular docking identified the potential interaction of ANQ with PI3K. Our data show for the first time that the bioactive component of A. camphorata, ANQ, suppresses stem cell-like properties via targeting PI3K/AKT/β-catenin signaling. ANQ could be a promising cancer prevention agent for colon cancer.

AB - Antroquinonol (ANQ) is a ubiquinone derivative from the unique mushroom Antrodia camphorata, which exhibits broad-spectrum bioactivities. The effects of ANQ on cancer stem cell-like properties in colon cancer, however, remain unclear. In this study, we found that ANQ inhibited growth of colon cancer cells. The 50% growth inhibitions (GI50) of ANQ on HCT15 and LoVo were 34.8 ± 0.07 and 17.9 ± 0.07 μM. Moreover, ANQ exhibited inhibitory activities toward migration/ invasion and tumorsphere formation of colon cancer cells. Mechanistically, ANQ inhibited pluripotent and cancer stem cellrelated genes and down-regulated β-catenin/T-cell factor (TCF) signaling. Moreover, activation of the phosphatidylinositol-3- kinase (PI3K)/AKT/β-catenin signaling axis was identified to be crucial for regulating the expressions of pluripotent genes, whereas suppression of PI3K/AKT by ANQ inhibited expressions of β-catenin and downstream targets. Molecular docking identified the potential interaction of ANQ with PI3K. Our data show for the first time that the bioactive component of A. camphorata, ANQ, suppresses stem cell-like properties via targeting PI3K/AKT/β-catenin signaling. ANQ could be a promising cancer prevention agent for colon cancer.

KW - Antrodia camphorata

KW - Antroquinonol

KW - Cancer prevention

KW - Colon cancer stem cell

KW - β-catenin

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U2 - 10.1021/acs.jafc.6b04101

DO - 10.1021/acs.jafc.6b04101

M3 - Article

VL - 65

SP - 51

EP - 59

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 1

ER -