Antrodia camphorata Potentiates Neuroprotection against Cerebral Ischemia in Rats via Downregulation of iNOS/HO-1/Bax and Activated Caspase-3 and Inhibition of Hydroxyl Radical Formation

Po Sheng Yang, Po Yen Lin, Chao Chien Chang, Meng Che Yu, Ting Lin Yen, Chang Chou Lan, Thanasekaran Jayakumar, Chih Hao Yang

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Abstract

Antrodia camphorata (A. camphorata) is a fungus generally used in Chinese folk medicine for treatment of viral hepatitis and cancer. Our previous study found A. camphorata has neuroprotective properties and could reduce stroke injury in cerebral ischemia animal models. In this study, we sought to investigate the molecular mechanisms of neuroprotective effects of A. camphorata in middle cerebral artery occlusion (MCAO) rats. A selective occlusion of the middle cerebral artery (MCA) with whole blood clots was used to induce ischemic stroke in rats and they were orally treated with A. camphorata (0.25 and 0.75 g/kg/day) alone or combined with aspirin (5 mg/kg/day). To provide insight into the functions of A. camphorata mediated neuroprotection, the expression of Bax, inducible nitric oxide synthase (iNOS), haem oxygenase-1 (HO-1), and activated caspase-3 was determined by Western blot assay. Treatment of aspirin alone significantly reduced the expressions of HO-1 (P•) formation at a dose of 40 mg/mL. Taken together, A. camphorata has shown neuroprotective effects in embolic rats, and the molecular mechanisms may correlate with the downregulation of Bax, iNOS, HO-1, and activated caspase-3 and the inhibition of OH signals.

Original languageEnglish
Article number232789
JournalEvidence-based Complementary and Alternative Medicine
Volume2015
DOIs
Publication statusPublished - 2015

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Antrodia
Heme Oxygenase (Decyclizing)
Nitric Oxide Synthase Type II
Brain Ischemia
Caspase 3
Hydroxyl Radical
Down-Regulation
Middle Cerebral Artery Infarction
Neuroprotective Agents
Aspirin
Stroke
Traditional Medicine
Hepatitis
Neuroprotection
Thrombosis
Fungi
Animal Models
Western Blotting
Wounds and Injuries
Therapeutics

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Antrodia camphorata Potentiates Neuroprotection against Cerebral Ischemia in Rats via Downregulation of iNOS/HO-1/Bax and Activated Caspase-3 and Inhibition of Hydroxyl Radical Formation. / Yang, Po Sheng; Lin, Po Yen; Chang, Chao Chien; Yu, Meng Che; Yen, Ting Lin; Lan, Chang Chou; Jayakumar, Thanasekaran; Yang, Chih Hao.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2015, 232789, 2015.

Research output: Contribution to journalArticle

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