Antitumor principle constituents of Myrica rubra var. acuminata

Ling-Ling Yang, Chia Chen Chang, Lih Geeng Chen, Ching Chiung Wang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Myrica rubra var. acuminata is a native shrub widely distributed and used as folk medicine in Taiwan for stomach disorders and diarrhea. Column chromatography combined with cytotoxic bioassay guided fractionation was performed to isolate the antitumor principles from fresh leaves of M. rubra var. acuminata. The 20% MeOH eluate fraction of M. rubra var. acuminata inhibited the viability of HeLa and P-388 cells in an in vitro assay and an in vivo P-388 tumor-bearing CDF1 mouse model. The percent increase in life span (%ILS) of 20% MeOH eluate fraction was greater than 125%. (-)Epigallocatechin 3-O-gallate (1) and prodelphinidin A-2,3′- O-gallate (2) were isolated from D-20 as the antitumor principle components. Both compounds can inhibit the growth of HeLa cells, but 1 had lower cytotoxic effects in normal cervical fibroblasts than did 2. Moreover, pretreatment with a caspase-3 specific inhibitor prevented 1- and 2-induced poly(ADP-ribose) polymerase cleavage. In view of these results, we suggest that 1 and 2 can induce apoptosis in HeLa cells and that activation of caspase-3 may provide a mechanistic explanation for their cytotoxic effects. Therefore, we suggest that the 20% MeOH eluate fraction extract is good for health and that M. rubra var, acuminata is an economically valuable plant.

Original languageEnglish
Pages (from-to)2974-2979
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number10
DOIs
Publication statusPublished - May 7 2003

Keywords

  • (-)epigallocatechin 3-O-gallate
  • Apoptosis
  • HeLa cells
  • Myrica rubra var. acuminata
  • Myricaceae
  • P-388 cells
  • Prodelphinidin A-2,3′-O-gallate

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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