Antitumor effects of zerumbone from Zingiber zerumbet in P-388D1 cells in vitro and in vivo

Guan Cheng Huang, Ting Yi Chien, Lih Geeng Chen, Ching Chiung Wang

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The fresh rhizome of Zingiber zerumbet (L.) Roscoe ex Smith (Zingiberaceae) is widely used as a folk medicine in Taiwan. In this study, the fresh rhizome was extracted with 95 % EtOH and partitioned with diethyl ether. The antitumor effects of the diethyl ether extract were measured in cultured P-388D 1 cells and in an animal model of P-388D1-bearing CDF 1 mice. The results indicated that the extract could induce DNA fragmentation in P-388D1 cells in vitro, and significantly prolong the life of P-388D1-bearing CDF1 mice (ILS% = 127.8) at a dosage of 5 mg/kg body weight. After column chromatography combined with an MTT cytotoxicity bioassay, zerumbone, a cyclic sesquiterpene was isolated from the diethyl ether extract. Zerumbone inhibited the growth of P-388D1 cells, induced DNA fragmentation in culture, and significantly prolonged the life of P-388D1-bearing CDF1 mice (1LS% = 120.5) at a dosage of 2 mg/kg. Furthermore, zerumbone inhibited the growth of a human leukemia cell line, HL-60 cells, in a time- and concentration-dependent manner, with IC50 values of 22.29, 9.12, and 2.27 μg/mL for 6, 12, and 18 h, respectively. The cell cycle of HL-60 cells was observed after treatment with zerumbone, which induced G2/M cell cycle arrest in HL-60 cells in a time- and concentration-dependent manner, and decreased the cyclin B1/cdk 1 protein level. These results suggest that zerumbone is an active principal of Z. zerumbet and is potentially a lead compound for the development of anticancer drugs.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalPlanta Medica
Volume71
Issue number3
DOIs
Publication statusPublished - Mar 2005

Keywords

  • Antitumor
  • Cell cycle
  • P-388D cell
  • Zerumbone
  • Zingiber zerumbet
  • Zingiberaceae

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

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