Antitumor effects of osthol from Cnidium monnieri

An in vitro and in vivo study

Szu-Yuan Chou, Chun-Sen Hsu, Kun Teng Wang, Min Chieh Wang, Ching Chiung Wang

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Cnidium monnieri (L.) Cusson is a Chinese medicine which is used widely by traditional medicine doctors. Osthol is a major bio-activity compound of the herb. In this study, osthol was isolated from C. monnieri and its in vitro and in vivo antitumor effects studied. The results of the in vitro study showed: that osthol inhibited the growth of HeLa, in a time- and concentration-dependent manner, with IC50, values of 77.96 and 64.94 μM for 24 and 48 h, respectively; that osthol had lower cytotoxic effects in primary cultured normal cervical fibroblasts; and that increased DNA fragmentation and activated PARP in HeLa after treatment with osthol which could induce apoptosis. The results of the in vivo model showed that the survival days of the P-388 D1 tumor-bearing CDF, mice were prolonged (ILS% = 37) after osthol (30 mg/kg) was given once a day for 9 days. Based on these results, it is suggested that osthol could inhibit P-388 D1 cells in vivo and induce apoptosis in HeLa cells in vitro, and that osthol is good lead compound for developing antitumor drugs. However, C. formosanum Yabe of Taiwan's endemic plants contained little osthol, with no imperatorin, and its major components were different from that of C. monnieri. Therefore, it is suggested that C. formosanum also may possess economic worth.

Original languageEnglish
Pages (from-to)226-230
Number of pages5
JournalPhytotherapy Research
Volume21
Issue number3
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Cnidium
Medicine
Bearings (structural)
Apoptosis
Lead compounds
In Vitro Techniques
osthol
Traditional Medicine
DNA Fragmentation
Fibroblasts
Bioactivity
Taiwan
HeLa Cells
Antineoplastic Agents
Inhibitory Concentration 50
Tumors

Keywords

  • Apoptosis
  • Cnidium formosanum
  • Cnidium monnieri
  • Osthol
  • P-388 D1-bearing CDF mice model
  • Umbelliferae

ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Antitumor effects of osthol from Cnidium monnieri : An in vitro and in vivo study. / Chou, Szu-Yuan; Hsu, Chun-Sen; Wang, Kun Teng; Wang, Min Chieh; Wang, Ching Chiung.

In: Phytotherapy Research, Vol. 21, No. 3, 03.2007, p. 226-230.

Research output: Contribution to journalArticle

Chou, Szu-Yuan ; Hsu, Chun-Sen ; Wang, Kun Teng ; Wang, Min Chieh ; Wang, Ching Chiung. / Antitumor effects of osthol from Cnidium monnieri : An in vitro and in vivo study. In: Phytotherapy Research. 2007 ; Vol. 21, No. 3. pp. 226-230.
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