Abstract

Isosteviol is a derivative of stevioside, a constituent of Stevia rebaudiana, which is commonly used as a noncaloric sugar substitute in Japan and Brazil. The aims of this study were to examine whether isosteviol alters angiotensin-II-induced cell proliferation in rat aortic smooth muscle cells. Cultured rat aortic smooth muscle cells were preincubated with isosteviol, then stimulated with angiotensin II, after which [3H]thymidine incorporation and endothelin-1 secretion were examined. Isosteviol (1-100 μmol/l) inhibits angiotensin-II-induced DNA synthesis and endothelin-1 secretion. Measurements of 2′7′-dichlorofluorescin diacetate, a redox-sensitive fluorescent dye, showed an isosteviol-mediated inhibition of intracellular reactive oxygen species generated by the effects of angiotensin II. The inductive properties of angiotensin II on extracellular signal-regulated kinase (ERK) phosphorylation were found reversed with isosteviol and antioxidants such as N-acetylcysteine. In summary, we speculate that isosteviol inhibits angiotensin-II-induced cell proliferation and endothelin-1 secretion via attenuation of reactive oxygen species generation. Thus, this study provides important insights that may contribute to the effects of isosteviol on the cardiovascular system.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalPharmacology
Volume76
Issue number4
DOIs
Publication statusPublished - Apr 2006

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Angiotensin II
Smooth Muscle Myocytes
Endothelin-1
Reactive Oxygen Species
Stevia
Cell Proliferation
Sweetening Agents
isosteviol
Extracellular Signal-Regulated MAP Kinases
Acetylcysteine
Cardiovascular System
Fluorescent Dyes
Thymidine
Oxidation-Reduction
Brazil
Japan
Antioxidants
Phosphorylation
DNA

Keywords

  • Angiotensin II
  • Endothelin-1
  • Isosteviol
  • Reactive oxygen species
  • Smooth muscle cells

ASJC Scopus subject areas

  • Pharmacology

Cite this

Antiproliferative effect of isosteviol on angiotensin-II-treated rat aortic smooth muscle cells. / Wong, Kar Lok; Lin, Jia Wei; Liu, Ju Chi; Yang, Hung Yu; Kao, Pei Feng; Chen, Cheng Hsien; Loh, Shih Hurng; Chiu, Wen Ta; Cheng, Tzu-Hurng; Lin, Jaung Geng; Hong, Hong Jye.

In: Pharmacology, Vol. 76, No. 4, 04.2006, p. 163-169.

Research output: Contribution to journalArticle

Wong, Kar Lok ; Lin, Jia Wei ; Liu, Ju Chi ; Yang, Hung Yu ; Kao, Pei Feng ; Chen, Cheng Hsien ; Loh, Shih Hurng ; Chiu, Wen Ta ; Cheng, Tzu-Hurng ; Lin, Jaung Geng ; Hong, Hong Jye. / Antiproliferative effect of isosteviol on angiotensin-II-treated rat aortic smooth muscle cells. In: Pharmacology. 2006 ; Vol. 76, No. 4. pp. 163-169.
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AU - Chen, Cheng Hsien

AU - Loh, Shih Hurng

AU - Chiu, Wen Ta

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