Antiproliferation of Cryptocarya concinna-derived cryptocaryone against oral cancer cells involving apoptosis, oxidative stress, and DNA damage

Hsun Shuo Chang, Jen Yang Tang, Ching Yu Yen, Hurng Wern Huang, Chang Yi Wu, Yi An Chung, Hui Ru Wang, Ih Sheng Chen, Ming Yii Huang, Hsueh Wei Chang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Cryptocarya-derived crude extracts and their compounds have been reported to have an antiproliferation effect on several types of cancers but their impact on oral cancer is less well understood. Methods: We examined the cell proliferation effect and mechanism of C. concinna-derived cryptocaryone (CPC) on oral cancer cells in terms of cell viability, apoptosis, reactive oxygen species (ROS), mitochondrial depolarization, and DNA damage. Results: We found that CPC dose-responsively reduced cell viability of two types of oral cancer cells (Ca9-22 and CAL 27) in MTS assay. The CPC-induced dose-responsive apoptosis effects on Ca9-22 cells were confirmed by flow cytometry-based sub-G1 accumulation, annexin V staining, and pancaspase analyses. For oral cancer Ca9-22 cells, CPC also induced oxidative stress responses in terms of ROS generation and mitochondrial depolarization. Moreover, γH2AX flow cytometry showed DNA damage in CPC-treated Ca9-22 cells. CPC-induced cell responses in terms of cell viability, apoptosis, oxidative stress, and DNA damage were rescued by N-acetylcysteine pretreatment, suggesting that oxidative stress plays an important role in CPC-induced death of oral cancer cells. Conclusions: CPC is a potential ROS-mediated natural product for anti-oral cancer therapy.

Original languageEnglish
Article number94
JournalBMC Complementary and Alternative Medicine
Volume16
Issue number1
DOIs
Publication statusPublished - Mar 8 2016

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Cryptocarya
Mouth Neoplasms
DNA Damage
Oxidative Stress
Apoptosis
Reactive Oxygen Species
Cell Survival
Flow Cytometry
cryptocaryone
Annexin A5
Acetylcysteine
Biological Products
Complex Mixtures
Mitochondrial DNA
Cell Proliferation

Keywords

  • Apoptosis
  • Cryptocarya concinna
  • Cryptocaryone
  • Oral cancer
  • Oxidative stress
  • γH2AX

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Antiproliferation of Cryptocarya concinna-derived cryptocaryone against oral cancer cells involving apoptosis, oxidative stress, and DNA damage. / Chang, Hsun Shuo; Tang, Jen Yang; Yen, Ching Yu; Huang, Hurng Wern; Wu, Chang Yi; Chung, Yi An; Wang, Hui Ru; Chen, Ih Sheng; Huang, Ming Yii; Chang, Hsueh Wei.

In: BMC Complementary and Alternative Medicine, Vol. 16, No. 1, 94, 08.03.2016.

Research output: Contribution to journalArticle

Chang, Hsun Shuo ; Tang, Jen Yang ; Yen, Ching Yu ; Huang, Hurng Wern ; Wu, Chang Yi ; Chung, Yi An ; Wang, Hui Ru ; Chen, Ih Sheng ; Huang, Ming Yii ; Chang, Hsueh Wei. / Antiproliferation of Cryptocarya concinna-derived cryptocaryone against oral cancer cells involving apoptosis, oxidative stress, and DNA damage. In: BMC Complementary and Alternative Medicine. 2016 ; Vol. 16, No. 1.
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abstract = "Background: Cryptocarya-derived crude extracts and their compounds have been reported to have an antiproliferation effect on several types of cancers but their impact on oral cancer is less well understood. Methods: We examined the cell proliferation effect and mechanism of C. concinna-derived cryptocaryone (CPC) on oral cancer cells in terms of cell viability, apoptosis, reactive oxygen species (ROS), mitochondrial depolarization, and DNA damage. Results: We found that CPC dose-responsively reduced cell viability of two types of oral cancer cells (Ca9-22 and CAL 27) in MTS assay. The CPC-induced dose-responsive apoptosis effects on Ca9-22 cells were confirmed by flow cytometry-based sub-G1 accumulation, annexin V staining, and pancaspase analyses. For oral cancer Ca9-22 cells, CPC also induced oxidative stress responses in terms of ROS generation and mitochondrial depolarization. Moreover, γH2AX flow cytometry showed DNA damage in CPC-treated Ca9-22 cells. CPC-induced cell responses in terms of cell viability, apoptosis, oxidative stress, and DNA damage were rescued by N-acetylcysteine pretreatment, suggesting that oxidative stress plays an important role in CPC-induced death of oral cancer cells. Conclusions: CPC is a potential ROS-mediated natural product for anti-oral cancer therapy.",
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AU - Huang, Hurng Wern

AU - Wu, Chang Yi

AU - Chung, Yi An

AU - Wang, Hui Ru

AU - Chen, Ih Sheng

AU - Huang, Ming Yii

AU - Chang, Hsueh Wei

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AB - Background: Cryptocarya-derived crude extracts and their compounds have been reported to have an antiproliferation effect on several types of cancers but their impact on oral cancer is less well understood. Methods: We examined the cell proliferation effect and mechanism of C. concinna-derived cryptocaryone (CPC) on oral cancer cells in terms of cell viability, apoptosis, reactive oxygen species (ROS), mitochondrial depolarization, and DNA damage. Results: We found that CPC dose-responsively reduced cell viability of two types of oral cancer cells (Ca9-22 and CAL 27) in MTS assay. The CPC-induced dose-responsive apoptosis effects on Ca9-22 cells were confirmed by flow cytometry-based sub-G1 accumulation, annexin V staining, and pancaspase analyses. For oral cancer Ca9-22 cells, CPC also induced oxidative stress responses in terms of ROS generation and mitochondrial depolarization. Moreover, γH2AX flow cytometry showed DNA damage in CPC-treated Ca9-22 cells. CPC-induced cell responses in terms of cell viability, apoptosis, oxidative stress, and DNA damage were rescued by N-acetylcysteine pretreatment, suggesting that oxidative stress plays an important role in CPC-induced death of oral cancer cells. Conclusions: CPC is a potential ROS-mediated natural product for anti-oral cancer therapy.

KW - Apoptosis

KW - Cryptocarya concinna

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