Antiproliferation and radiosensitization of caffeic acid phenethyl ester on human medulloblastoma cells

Yi Hsien Lin, Jen Hwey Chiu, Wen Ser Tseng, Tai-Tong Wong, Shih Hwa Chiou, Sang Hue Yen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Purpose: To investigate antiproliferative and radiosensitizing effects of caffeic acid phenethyl ester (CAPE) on medulloblastoma (MB) Daoy cells. Methods and materials: Daoy cells were treated with CAPE in different concentrations and assessed for cell viability, apoptosis, cell cycles, cyclin Bl expressions, radio-sensitization and chemosensitization. Human astroglia SVGp12 cells were treated with CAPE to present the possible protection or complication effects in normal tissues. Results: CAPE inhibited the growth of Daoy cells in a time- and concentration-dependent manner in MTT and Trypan blue exclusion assays. Flow cytometry revealed that CAPE significantly decreased G2/M fraction, and increased the S phase fraction. Western blot demonstrated a down-regulated cyclin B1 protein expression. Pretreatment with CAPE markedly decreased the viability of irradiated Daoy cells. The sensitizer enhancement ratios (SERs) were increased in CAPE-treated Daoy cells. CAPE in doxorubicin and cisplatin did not show chemosensitizing effect. Conclusions: These findings demonstrate the antiproliferative and radiosensitizing effects of CAPE for Daoy cells, which might bring improvement to the treatment of MB. For clinical application, in vivo models are expected.

Original languageEnglish
Pages (from-to)525-532
Number of pages8
JournalCancer Chemotherapy and Pharmacology
Volume57
Issue number4
DOIs
Publication statusPublished - Apr 1 2006
Externally publishedYes

Fingerprint

Medulloblastoma
Radiation-Sensitizing Agents
Cells
Cyclin B1
caffeic acid phenethyl ester
Cyclins
Trypan Blue
Flow cytometry
Radio
S Phase
Astrocytes
Doxorubicin
Cisplatin
Assays
Cell Survival
Cell Cycle
Flow Cytometry
Western Blotting
Tissue
Apoptosis

Keywords

  • Antiproliferation
  • Caffeic acid phenethyl ester (CAPE)
  • Medulloblastoma
  • Propolis
  • Radiosensitization
  • Radiotherapy

ASJC Scopus subject areas

  • Oncology
  • Toxicology
  • Pharmacology
  • Cancer Research
  • Pharmacology (medical)

Cite this

Antiproliferation and radiosensitization of caffeic acid phenethyl ester on human medulloblastoma cells. / Lin, Yi Hsien; Chiu, Jen Hwey; Tseng, Wen Ser; Wong, Tai-Tong; Chiou, Shih Hwa; Yen, Sang Hue.

In: Cancer Chemotherapy and Pharmacology, Vol. 57, No. 4, 01.04.2006, p. 525-532.

Research output: Contribution to journalArticle

Lin, Yi Hsien ; Chiu, Jen Hwey ; Tseng, Wen Ser ; Wong, Tai-Tong ; Chiou, Shih Hwa ; Yen, Sang Hue. / Antiproliferation and radiosensitization of caffeic acid phenethyl ester on human medulloblastoma cells. In: Cancer Chemotherapy and Pharmacology. 2006 ; Vol. 57, No. 4. pp. 525-532.
@article{205fce166fb8493cafc0975bb484b921,
title = "Antiproliferation and radiosensitization of caffeic acid phenethyl ester on human medulloblastoma cells",
abstract = "Purpose: To investigate antiproliferative and radiosensitizing effects of caffeic acid phenethyl ester (CAPE) on medulloblastoma (MB) Daoy cells. Methods and materials: Daoy cells were treated with CAPE in different concentrations and assessed for cell viability, apoptosis, cell cycles, cyclin Bl expressions, radio-sensitization and chemosensitization. Human astroglia SVGp12 cells were treated with CAPE to present the possible protection or complication effects in normal tissues. Results: CAPE inhibited the growth of Daoy cells in a time- and concentration-dependent manner in MTT and Trypan blue exclusion assays. Flow cytometry revealed that CAPE significantly decreased G2/M fraction, and increased the S phase fraction. Western blot demonstrated a down-regulated cyclin B1 protein expression. Pretreatment with CAPE markedly decreased the viability of irradiated Daoy cells. The sensitizer enhancement ratios (SERs) were increased in CAPE-treated Daoy cells. CAPE in doxorubicin and cisplatin did not show chemosensitizing effect. Conclusions: These findings demonstrate the antiproliferative and radiosensitizing effects of CAPE for Daoy cells, which might bring improvement to the treatment of MB. For clinical application, in vivo models are expected.",
keywords = "Antiproliferation, Caffeic acid phenethyl ester (CAPE), Medulloblastoma, Propolis, Radiosensitization, Radiotherapy",
author = "Lin, {Yi Hsien} and Chiu, {Jen Hwey} and Tseng, {Wen Ser} and Tai-Tong Wong and Chiou, {Shih Hwa} and Yen, {Sang Hue}",
year = "2006",
month = "4",
day = "1",
doi = "10.1007/s00280-005-0066-8",
language = "English",
volume = "57",
pages = "525--532",
journal = "Cancer Chemotherapy and Pharmacology",
issn = "0344-5704",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Antiproliferation and radiosensitization of caffeic acid phenethyl ester on human medulloblastoma cells

AU - Lin, Yi Hsien

AU - Chiu, Jen Hwey

AU - Tseng, Wen Ser

AU - Wong, Tai-Tong

AU - Chiou, Shih Hwa

AU - Yen, Sang Hue

PY - 2006/4/1

Y1 - 2006/4/1

N2 - Purpose: To investigate antiproliferative and radiosensitizing effects of caffeic acid phenethyl ester (CAPE) on medulloblastoma (MB) Daoy cells. Methods and materials: Daoy cells were treated with CAPE in different concentrations and assessed for cell viability, apoptosis, cell cycles, cyclin Bl expressions, radio-sensitization and chemosensitization. Human astroglia SVGp12 cells were treated with CAPE to present the possible protection or complication effects in normal tissues. Results: CAPE inhibited the growth of Daoy cells in a time- and concentration-dependent manner in MTT and Trypan blue exclusion assays. Flow cytometry revealed that CAPE significantly decreased G2/M fraction, and increased the S phase fraction. Western blot demonstrated a down-regulated cyclin B1 protein expression. Pretreatment with CAPE markedly decreased the viability of irradiated Daoy cells. The sensitizer enhancement ratios (SERs) were increased in CAPE-treated Daoy cells. CAPE in doxorubicin and cisplatin did not show chemosensitizing effect. Conclusions: These findings demonstrate the antiproliferative and radiosensitizing effects of CAPE for Daoy cells, which might bring improvement to the treatment of MB. For clinical application, in vivo models are expected.

AB - Purpose: To investigate antiproliferative and radiosensitizing effects of caffeic acid phenethyl ester (CAPE) on medulloblastoma (MB) Daoy cells. Methods and materials: Daoy cells were treated with CAPE in different concentrations and assessed for cell viability, apoptosis, cell cycles, cyclin Bl expressions, radio-sensitization and chemosensitization. Human astroglia SVGp12 cells were treated with CAPE to present the possible protection or complication effects in normal tissues. Results: CAPE inhibited the growth of Daoy cells in a time- and concentration-dependent manner in MTT and Trypan blue exclusion assays. Flow cytometry revealed that CAPE significantly decreased G2/M fraction, and increased the S phase fraction. Western blot demonstrated a down-regulated cyclin B1 protein expression. Pretreatment with CAPE markedly decreased the viability of irradiated Daoy cells. The sensitizer enhancement ratios (SERs) were increased in CAPE-treated Daoy cells. CAPE in doxorubicin and cisplatin did not show chemosensitizing effect. Conclusions: These findings demonstrate the antiproliferative and radiosensitizing effects of CAPE for Daoy cells, which might bring improvement to the treatment of MB. For clinical application, in vivo models are expected.

KW - Antiproliferation

KW - Caffeic acid phenethyl ester (CAPE)

KW - Medulloblastoma

KW - Propolis

KW - Radiosensitization

KW - Radiotherapy

UR - http://www.scopus.com/inward/record.url?scp=30644463358&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30644463358&partnerID=8YFLogxK

U2 - 10.1007/s00280-005-0066-8

DO - 10.1007/s00280-005-0066-8

M3 - Article

VL - 57

SP - 525

EP - 532

JO - Cancer Chemotherapy and Pharmacology

JF - Cancer Chemotherapy and Pharmacology

SN - 0344-5704

IS - 4

ER -