Abstract

In this study, Gram-positive Staphylococcus aureus lipoteichoic acid (LTA) dose dependently (0.1-1.0 μg/mL) and time dependently (10-60 min) inhibited platelet aggregation in human platelets stimulated by agonists (i.e., thrombin and collagen). LTA also dose dependently inhibited intracellular Ca2+ mobilization in human platelets stimulated by collagen. In addition, LTA (0.5 and 1.0 μg/mL) dose dependently increased the formation of cyclic AMP but not cyclic GMP in platelets. LTA (0.5 and 1.0 μg/mL) did not significantly increase the production of nitrate within a 10-min incubation period. Rapid phosphorylation of a platelet protein of M(r) 47,000, a marker of protein kinase C activation, was triggered by PDBu (0.03 μM). This phosphorylation was dose dependently inhibited by LTA (0.5 and 1.0 μg/mL) within a 10-min incubation period. Furthermore, LTA (0.5 and 1.0 μg/mL) also inhibited platelet aggregation induced by PDBu (0.03 μM) in human platelets.These results indicate that the antiplatelet activity of LTA may be involved in the increase of cyclic AMP, leading to inhibition of intracellular Ca2+ mobilization and protein kinase C activity. Therefore, LTA-mediated alteration of platelet function may contribute to bleeding diathesis in septicemic and endotoxemic patients. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)249-258
Number of pages10
JournalThrombosis Research
Volume99
Issue number3
DOIs
Publication statusPublished - Jun 1 2000

Fingerprint

Cyclic AMP
Staphylococcus aureus
Blood Platelets
Platelet Aggregation
Protein Kinase C
Collagen
Phosphorylation
lipoteichoic acid
Disease Susceptibility
Cyclic GMP
Thrombin
Nitrates
Hemorrhage
Proteins

Keywords

  • Cyclic AMP
  • Gram-positive bacteria
  • LTA
  • Platelet aggregation
  • Protein kinase C

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Antiplatelet activity of Staphylococcus aureus lipoteichoic acid is mediated through a cyclic AMP pathway. / Sheu, Joen Rong; Hsiao, George; Lee, Cheng Rong; Chang, Wen Chiung; Lee, Ling Wen; Su, Ching Hua; Lin, Chien-Huang.

In: Thrombosis Research, Vol. 99, No. 3, 01.06.2000, p. 249-258.

Research output: Contribution to journalArticle

Sheu, Joen Rong ; Hsiao, George ; Lee, Cheng Rong ; Chang, Wen Chiung ; Lee, Ling Wen ; Su, Ching Hua ; Lin, Chien-Huang. / Antiplatelet activity of Staphylococcus aureus lipoteichoic acid is mediated through a cyclic AMP pathway. In: Thrombosis Research. 2000 ; Vol. 99, No. 3. pp. 249-258.
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N2 - In this study, Gram-positive Staphylococcus aureus lipoteichoic acid (LTA) dose dependently (0.1-1.0 μg/mL) and time dependently (10-60 min) inhibited platelet aggregation in human platelets stimulated by agonists (i.e., thrombin and collagen). LTA also dose dependently inhibited intracellular Ca2+ mobilization in human platelets stimulated by collagen. In addition, LTA (0.5 and 1.0 μg/mL) dose dependently increased the formation of cyclic AMP but not cyclic GMP in platelets. LTA (0.5 and 1.0 μg/mL) did not significantly increase the production of nitrate within a 10-min incubation period. Rapid phosphorylation of a platelet protein of M(r) 47,000, a marker of protein kinase C activation, was triggered by PDBu (0.03 μM). This phosphorylation was dose dependently inhibited by LTA (0.5 and 1.0 μg/mL) within a 10-min incubation period. Furthermore, LTA (0.5 and 1.0 μg/mL) also inhibited platelet aggregation induced by PDBu (0.03 μM) in human platelets.These results indicate that the antiplatelet activity of LTA may be involved in the increase of cyclic AMP, leading to inhibition of intracellular Ca2+ mobilization and protein kinase C activity. Therefore, LTA-mediated alteration of platelet function may contribute to bleeding diathesis in septicemic and endotoxemic patients. Copyright (C) 2000 Elsevier Science Ltd.

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