Abstract

Antrodia camphorata (A. camphorata) is well-known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of A. camphorata extracts to protect against oxidative stress in vitro and against carbon tetrachloride (CCl4)-induced hepatic injury in vivo. An extract of A. camphorata inhibited nonenzymatic iron-induced lipid peroxidation in rat brain homogenates with an IC50 value about 3.1 mg/mL. It also scavenged the stable free radical 1,1diphenyl-2-picrylhydrazyl (DPPH). The dose of the A. camphorata extract resulting in a decrease of 0.20 in the absorbance of DPPH was about 31 ± 0.7 μg/mL. Furthermore, an A. camphorata extract dose-dependently (250-1250 mg/kg) ameliorated the increase in plasma aspartate aminotransferase (GOT) and alanine aminotransferase (GPT) levels caused by chronic repeated CCl4 intoxication in mice. Moreover, A. camphorata extract significantly improved the CCl4-induced increase in hepatic glutathione peroxidase, reductase, and CCl4-induced decrease in superoxide dismutase activities. It also restored the decrement in the glutathione content and catalase activity of hepatic tissues in CCl4-intoxicated mice. Furthermore, it also dose-dependently inhibited the formation of lipid peroxidative products during CCl4 treatment. Histopathological changes of hepatic lesions induced by CCl4 were significantly ameliorated by treatment with an A. camphorata extract in a dose-dependent manner. These results suggest that A. camphorata extract exerts effective protection against chronic chemical-induced hepatic injury in vivo, by mediating antioxidative and free radical scavenging activities.

Original languageEnglish
Pages (from-to)3302-3308
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number11
DOIs
Publication statusPublished - May 21 2003

Fingerprint

Antrodia
Taiwanofungus camphoratus
hepatoprotective effect
Free Radicals
Lipids
Oxidative stress
Carbon Tetrachloride
Scavenging
extracts
Glutathione Peroxidase
Aspartate Aminotransferases
Alanine Transaminase
Catalase
Superoxide Dismutase
Medicine
Glutathione
Rats
Brain
Oxidoreductases
Iron

Keywords

  • Antioxidant
  • Antrodia camphorata
  • Carbon tetrachloride
  • Free radical scavenger
  • Hepatotoxicity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Antioxidative and hepatoprotective effects of Antrodia camphorata extract. / Hsiao, George; Shen, Ming Yi; Lin, Kuan Hung; Lan, Ming Huang; Wu, Li Yu; Chou, Duen Suey; Lin, Chien-Huang; Su, Ching Hua; Sheu, Joen Rong.

In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 11, 21.05.2003, p. 3302-3308.

Research output: Contribution to journalArticle

Hsiao, George ; Shen, Ming Yi ; Lin, Kuan Hung ; Lan, Ming Huang ; Wu, Li Yu ; Chou, Duen Suey ; Lin, Chien-Huang ; Su, Ching Hua ; Sheu, Joen Rong. / Antioxidative and hepatoprotective effects of Antrodia camphorata extract. In: Journal of Agricultural and Food Chemistry. 2003 ; Vol. 51, No. 11. pp. 3302-3308.
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abstract = "Antrodia camphorata (A. camphorata) is well-known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of A. camphorata extracts to protect against oxidative stress in vitro and against carbon tetrachloride (CCl4)-induced hepatic injury in vivo. An extract of A. camphorata inhibited nonenzymatic iron-induced lipid peroxidation in rat brain homogenates with an IC50 value about 3.1 mg/mL. It also scavenged the stable free radical 1,1diphenyl-2-picrylhydrazyl (DPPH). The dose of the A. camphorata extract resulting in a decrease of 0.20 in the absorbance of DPPH was about 31 ± 0.7 μg/mL. Furthermore, an A. camphorata extract dose-dependently (250-1250 mg/kg) ameliorated the increase in plasma aspartate aminotransferase (GOT) and alanine aminotransferase (GPT) levels caused by chronic repeated CCl4 intoxication in mice. Moreover, A. camphorata extract significantly improved the CCl4-induced increase in hepatic glutathione peroxidase, reductase, and CCl4-induced decrease in superoxide dismutase activities. It also restored the decrement in the glutathione content and catalase activity of hepatic tissues in CCl4-intoxicated mice. Furthermore, it also dose-dependently inhibited the formation of lipid peroxidative products during CCl4 treatment. Histopathological changes of hepatic lesions induced by CCl4 were significantly ameliorated by treatment with an A. camphorata extract in a dose-dependent manner. These results suggest that A. camphorata extract exerts effective protection against chronic chemical-induced hepatic injury in vivo, by mediating antioxidative and free radical scavenging activities.",
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AU - Wu, Li Yu

AU - Chou, Duen Suey

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AU - Sheu, Joen Rong

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