Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats

Yen-Hsien Lee, Jung-Jhih Chang, Chiang-Ting Chien, Ming-Chien Yang, Hsiung-Fei Chien

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer. © 2012 Yen-Hsien Lee et al.
Original languageEnglish
JournalExperimental Diabetes Research
Volume2012
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

UCON 50-HB-5100
Poloxamer
Polymethyl Methacrylate
Streptozocin
Wound Healing
Antioxidants
Ascorbic Acid
Gels
Skin
Wounds and Injuries
Collagen
Apoptosis
Drug Delivery Systems
Wistar Rats
Air

Keywords

  • ascorbic acid
  • collagen
  • poloxamer
  • sodium chloride
  • antioxidant
  • hydroxyproline
  • reactive oxygen metabolite
  • streptozocin
  • animal cell
  • animal experiment
  • animal model
  • animal tissue
  • antioxidant activity
  • apoptosis
  • article
  • collagen synthesis
  • controlled study
  • dermis
  • drug delivery system
  • drug effect
  • epidermis
  • female
  • in vitro study
  • nonhuman
  • priority journal
  • protein content
  • rat
  • skin injury
  • streptozocin diabetes
  • tissue regeneration
  • wound contraction
  • wound healing
  • animal
  • chemistry
  • experimental diabetes mellitus
  • immunohistochemistry
  • metabolism
  • methodology
  • pathology
  • pathophysiology
  • phase transition
  • skin
  • time
  • Wistar rat
  • Animals
  • Antioxidants
  • Apoptosis
  • Ascorbic Acid
  • Diabetes Mellitus, Experimental
  • Drug Delivery Systems
  • Female
  • Hydroxyproline
  • Immunohistochemistry
  • Phase Transition
  • Poloxamer
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species
  • Skin
  • Streptozocin
  • Time Factors
  • Wound Healing

Cite this

Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats. / Lee, Yen-Hsien; Chang, Jung-Jhih; Chien, Chiang-Ting; Yang, Ming-Chien; Chien, Hsiung-Fei.

In: Experimental Diabetes Research, Vol. 2012, 2012.

Research output: Contribution to journalArticle

Lee, Yen-Hsien ; Chang, Jung-Jhih ; Chien, Chiang-Ting ; Yang, Ming-Chien ; Chien, Hsiung-Fei. / Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats. In: Experimental Diabetes Research. 2012 ; Vol. 2012.
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title = "Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats",
abstract = "We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer. {\circledC} 2012 Yen-Hsien Lee et al.",
keywords = "ascorbic acid, collagen, poloxamer, sodium chloride, antioxidant, hydroxyproline, reactive oxygen metabolite, streptozocin, animal cell, animal experiment, animal model, animal tissue, antioxidant activity, apoptosis, article, collagen synthesis, controlled study, dermis, drug delivery system, drug effect, epidermis, female, in vitro study, nonhuman, priority journal, protein content, rat, skin injury, streptozocin diabetes, tissue regeneration, wound contraction, wound healing, animal, chemistry, experimental diabetes mellitus, immunohistochemistry, metabolism, methodology, pathology, pathophysiology, phase transition, skin, time, Wistar rat, Animals, Antioxidants, Apoptosis, Ascorbic Acid, Diabetes Mellitus, Experimental, Drug Delivery Systems, Female, Hydroxyproline, Immunohistochemistry, Phase Transition, Poloxamer, Rats, Rats, Wistar, Reactive Oxygen Species, Skin, Streptozocin, Time Factors, Wound Healing",
author = "Yen-Hsien Lee and Jung-Jhih Chang and Chiang-Ting Chien and Ming-Chien Yang and Hsiung-Fei Chien",
note = "被引用次數:10 Export Date: 16 March 2016 通訊地址: Yang, M.-C.; Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan; 電子郵件: myang@mail.ntust.edu.tw 化學物質/CAS: ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; collagen, 9007-34-5; poloxamer, 9003-11-6; sodium chloride, 7647-14-5; hydroxyproline, 51-35-4, 6912-67-0; streptozocin, 18883-66-4; Antioxidants; Ascorbic Acid, 50-81-7; Hydroxyproline, 51-35-4; Poloxamer, 106392-12-5; Reactive Oxygen Species; Streptozocin, 18883-66-4 製造商: Sigma Aldrich, United States 參考文獻: Singer, A.J., Clark, R.A.F., Cutaneous wound healing (1999) New England Journal of Medicine, 341 (10), pp. 738-746. , DOI 10.1056/NEJM199909023411006; Luo, J.-D., Wang, Y.-Y., Fu, W.-L., Wu, J., Chen, A.F., Gene therapy of endothelial nitric oxide synthase and manganese superoxide dismutase restores delayed wound healing in type 1 diabetic mice (2004) Circulation, 110 (16), pp. 2484-2493. , DOI 10.1161/01.CIR.0000137969.87365.05; Schorah, C.J., Downing, C., Piripitsi, A., Gallivan, L., Al-Hazaa, A.H., Sanderson, M.J., Bodenham, A., Total vitamin C, ascorbic acid, and dehydroascorbic acid concentrations in plasma of critically ill patients (1996) American Journal of Clinical Nutrition, 63 (5), pp. 760-765; Clark, R.A.F., Oxidative stress and senescent fibroblasts in non-healing wounds as potential therapeutic targets (2008) Journal of Investigative Dermatology, 128 (10), pp. 2361-2364. , 2-s2.0-51749092207 10.1038/jid.2008.257; Schneir, M., Ramamurthy, N., Golub, L., Dietary ascorbic acid normalizes diabetes-induced underhydroxylation of nascent type I collagen molecules (1985) Collagen and Related Research, 5 (5), pp. 415-422; Briganti, S., Picardo, M., Antioxidant activity, lipid peroxidation and skin diseases. What's new (2003) Journal of the European Academy of Dermatology and Venereology, 17 (6), pp. 663-669. , DOI 10.1046/j.1468-3083.2003.00751.x; Riccioni, G., Bucciarelli, T., Mancini, B., Di Ilio, C., Capra, V., D'Orazio, N., The role of the antioxidant vitamin supplementation in the prevention of cardiovascular diseases (2007) Expert Opinion on Investigational Drugs, 16 (1), pp. 25-32. , DOI 10.1517/13543784.16.1.25; Phillips, C.L., Combs, S.B., Pinnell, S.R., Effects of ascorbic acid on proliferation and collagen synthesis in relation to the donor age of human dermal fibroblasts (1994) Journal of Investigative Dermatology, 103 (2), pp. 228-232; Bickers, D.R., Athar, M., Oxidative stress in the pathogenesis of skin disease (2006) Journal of Investigative Dermatology, 126 (12), pp. 2565-2575. , DOI 10.1038/sj.jid.5700340, PII 5700340; Silvetti, A.N., An effective method of treating long-enduring wounds and ulcers by topical applications of solutions of nutrients (1981) Journal of Dermatologic Surgery and Oncology, 7 (6), pp. 501-508; Collins, N., Adding vitamin C to the wound management mix (2004) Advances in Skin & Wound Care, 17 (3), pp. 109-112. , 2-s2.0-3142628477; Bercea, M., Darie, R.N., Nita, L.E., Morariu, S., Temperature responsive gels based on Pluronic F127 and poly(vinyl alcohol) (2011) Industrial and Engineering Chemistry Research, 50 (7), pp. 4199-4206. , 2-s2.0-79953293749 10.1021/ie1024408; Schmolka, I.R., Artificial skin. I. Preparation and properties of pluronic F-127 gels for treatment of burns. (1972) Journal of Biomedical Materials Research, 6 (6), pp. 571-582. , 2-s2.0-0015420938; Bromberg, L.E., Ron, E.S., Temperature-responsive gels and thermogelling polymer matrices for protein and peptide delivery (1998) Advanced Drug Delivery Reviews, 31 (3), pp. 197-221. , DOI 10.1016/S0169-409X(97)00121-X, PII S0169409X9700121X; Sharma, P.K., Reilly, M.J., Jones, D.N., Robinson, P.M., Bhatia, S.R., The effect of pharmaceuticals on the nanoscale structure of PEO-PPO-PEO micelles (2008) Colloids and Surfaces B: Biointerfaces, 61 (1), pp. 53-60. , DOI 10.1016/j.colsurfb.2007.07.002, PII S0927776507002822; Chang, J.J., Lin, P.J., Lee, Y.H., Yang, M.C., Chien, C.T., The effect of covalent immobilization of sialic acid on the removal of lipopolysaccharide and reactive oxygen species for polyethylene terephthalate (2011) Polymer for Advanced Technologies, 22, pp. 1872-1878; Baoyong, L., Jian, Z., Denglong, C., Min, L., Evaluation of a new type of wound dressing made from recombinant spider silk protein using rat models (2010) Burns, 36 (6), pp. 891-896. , 2-s2.0-77955588339 10.1016/j.burns.2009.12.001; Yates, C.C., Whaley, D., Babu, R., Zhang, J., Krishna, P., Beckman, E., Pasculle, A.W., Wells, A., The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse skin wound models (2007) Biomaterials, 28 (27), pp. 3977-3986. , DOI 10.1016/j.biomaterials.2007.05.008, PII S0142961207004048; Nemes, Z., Steinert, P.M., Bricks and mortar of the epidermal barrier (1999) Experimental and Molecular Medicine, 31 (1), pp. 5-19; Candi, E., Schmidt, R., Melino, G., The cornified envelope: A model of cell death in the skin (2005) Nature Reviews Molecular Cell Biology, 6 (4), pp. 328-340. , DOI 10.1038/nrm1619; Yamaoka, T., Takahashi, Y., Fujisato, T., Lee, C.W., Tsuji, T., Ohta, T., Murakami, A., Kimura, Y., Novel adhesion prevention membrane based on a bioresorbable copoly(ester-ether) comprised of poly-l-lactide and pluronic{\circledR}: In vitro and in vivo evaluations (2001) Journal of Biomedical Materials Research, 54 (4), pp. 470-479. , DOI 10.1002/1097-4636(20010315)54:4<470::AID-JBM20>3.0.CO;2-X; Oh, S.H., Kim, J.K., Song, K.S., Noh, S.M., Ghil, S.H., Yuk, S.H., Lee, J.H., Prevention of postsurgical tissue adhesion by anti-inflammatory drug-loaded Pluronic mixtures with sol-gel transition behavior (2005) Journal of Biomedical Materials Research - Part A, 72 (3), pp. 306-316. , DOI 10.1002/jbm.a.30239; Hokett, S.D., Cuenin, M.F., O'Neal, R.B., Brennan, W.A., Strong, S.L., Runner, R.R., McPherson, J.C., Van Dyke, T.E., Pluronic polyol effects on human gingival fibroblast attachment and growth (2000) Journal of Periodontology, 71 (5), pp. 803-809; Fowler, E.B., Cuenin, M.F., Hokett, S.D., Peacock, M.E., McPherson III, J.C., Dirksen, T.R., Sharawy, M., Billman, M.A., Evaluation of pluronic polyols as carriers for grafting materials: Study in rat calvaria defects (2002) Journal of Periodontology, 73 (2), pp. 191-197. , DOI 10.1902/jop.2002.73.2.191; Khalil, E.A., Afifi, F.U., Al-Hussaini, M., Evaluation of the wound healing effect of some Jordanian traditional medicinal plants formulated in Pluronic F127 using mice (Mus musculus) (2007) Journal of Ethnopharmacology, 109 (1), pp. 104-112. , DOI 10.1016/j.jep.2006.07.010, PII S0378874106003473; Abd-El-Aleem, S.A., Ferguson, M.W.J., Appleton, I., Kairsingh, S., Jude, E.B., Jones, K., McCollum, C.N., Ireland, G.W., Expression of nitric oxide synthase isoforms and arginase in normal human skin and chronic venous leg ulcers (2000) Journal of Pathology, 191 (4), pp. 434-442; Rojkind, M., Dominguez-Rosales, J.-A., Nieto, N., Greenwel, P., Role of hydrogen peroxide and oxidative stress in healing responses (2002) Cellular and Molecular Life Sciences, 59 (11), pp. 1872-1891. , DOI 10.1007/PL00012511; Moseley, R., Hilton, J.R., Waddington, R.J., Harding, K.G., Stephens, P., Thomas, D.W., Comparison of oxidative stress biomarker profiles between acute and chronic wound environments (2004) Wound Repair and Regeneration, 12 (4), pp. 419-429. , DOI 10.1111/j.1067-1927.2004.12406.x; Rasik, A.M., Shukla, A., Antioxidant status in delayed healing type of wounds (2000) International Journal of Experimental Pathology, 81 (4), pp. 257-263. , 2-s2.0-0033864199 10.1046/j.1365-2613.2000.00158.x; Gopinath, D., Ahmed, M.R., Gomathi, K., Chitra, K., Sehgal, P.K., Jayakumar, R., Dermal wound healing processes with curcumin incorporated collagen films (2004) Biomaterials, 25 (10), pp. 1911-1917. , DOI 10.1016/S0142-9612(03)00625-2; Khanna, S., Roy, S., Bagchi, D., Bagchi, M., Sen, C.K., Upregulation of oxidant-induced VEGF expression in cultured keratinocytes by a grape seed proanthocyanidin extract (2001) Free Radical Biology and Medicine, 31 (1), pp. 38-42. , DOI 10.1016/S0891-5849(01)00544-5, PII S0891584901005445; Fadini, G.P., Albiero, M., Menegazzo, L., Boscaro, E., Pagnin, E., Iori, E., Cosma, C., Avogaro, A., The redox enzyme p66Shc contributes to diabetes and ischemia-induced delay in cutaneous wound healing (2010) Diabetes, 59 (9), pp. 2306-2314. , 2-s2.0-77956379343 10.2337/db09-1727; Al-Mashat, H.A., Kandru, S., Liu, R., Behl, Y., Desta, T., Graves, D.T., Diabetes enhances mRNA levels of proapoptotic genes and caspase activity, which contribute to impaired healing (2006) Diabetes, 55 (2), pp. 487-495. , http://diabetes.diabetesjournals.org/cgi/reprint/55/2/487, DOI 10.2337/diabetes.55.02.06.db05-1201",
year = "2012",
doi = "10.1155/2012/504693",
language = "English",
volume = "2012",
journal = "Experimental Diabesity Research",
issn = "1543-8600",
publisher = "Taylor and Francis Ltd.",

}

TY - JOUR

T1 - Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats

AU - Lee, Yen-Hsien

AU - Chang, Jung-Jhih

AU - Chien, Chiang-Ting

AU - Yang, Ming-Chien

AU - Chien, Hsiung-Fei

N1 - 被引用次數:10 Export Date: 16 March 2016 通訊地址: Yang, M.-C.; Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan; 電子郵件: myang@mail.ntust.edu.tw 化學物質/CAS: ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; collagen, 9007-34-5; poloxamer, 9003-11-6; sodium chloride, 7647-14-5; hydroxyproline, 51-35-4, 6912-67-0; streptozocin, 18883-66-4; Antioxidants; Ascorbic Acid, 50-81-7; Hydroxyproline, 51-35-4; Poloxamer, 106392-12-5; Reactive Oxygen Species; Streptozocin, 18883-66-4 製造商: Sigma Aldrich, United States 參考文獻: Singer, A.J., Clark, R.A.F., Cutaneous wound healing (1999) New England Journal of Medicine, 341 (10), pp. 738-746. , DOI 10.1056/NEJM199909023411006; Luo, J.-D., Wang, Y.-Y., Fu, W.-L., Wu, J., Chen, A.F., Gene therapy of endothelial nitric oxide synthase and manganese superoxide dismutase restores delayed wound healing in type 1 diabetic mice (2004) Circulation, 110 (16), pp. 2484-2493. , DOI 10.1161/01.CIR.0000137969.87365.05; Schorah, C.J., Downing, C., Piripitsi, A., Gallivan, L., Al-Hazaa, A.H., Sanderson, M.J., Bodenham, A., Total vitamin C, ascorbic acid, and dehydroascorbic acid concentrations in plasma of critically ill patients (1996) American Journal of Clinical Nutrition, 63 (5), pp. 760-765; Clark, R.A.F., Oxidative stress and senescent fibroblasts in non-healing wounds as potential therapeutic targets (2008) Journal of Investigative Dermatology, 128 (10), pp. 2361-2364. , 2-s2.0-51749092207 10.1038/jid.2008.257; Schneir, M., Ramamurthy, N., Golub, L., Dietary ascorbic acid normalizes diabetes-induced underhydroxylation of nascent type I collagen molecules (1985) Collagen and Related Research, 5 (5), pp. 415-422; Briganti, S., Picardo, M., Antioxidant activity, lipid peroxidation and skin diseases. What's new (2003) Journal of the European Academy of Dermatology and Venereology, 17 (6), pp. 663-669. , DOI 10.1046/j.1468-3083.2003.00751.x; Riccioni, G., Bucciarelli, T., Mancini, B., Di Ilio, C., Capra, V., D'Orazio, N., The role of the antioxidant vitamin supplementation in the prevention of cardiovascular diseases (2007) Expert Opinion on Investigational Drugs, 16 (1), pp. 25-32. , DOI 10.1517/13543784.16.1.25; Phillips, C.L., Combs, S.B., Pinnell, S.R., Effects of ascorbic acid on proliferation and collagen synthesis in relation to the donor age of human dermal fibroblasts (1994) Journal of Investigative Dermatology, 103 (2), pp. 228-232; Bickers, D.R., Athar, M., Oxidative stress in the pathogenesis of skin disease (2006) Journal of Investigative Dermatology, 126 (12), pp. 2565-2575. , DOI 10.1038/sj.jid.5700340, PII 5700340; Silvetti, A.N., An effective method of treating long-enduring wounds and ulcers by topical applications of solutions of nutrients (1981) Journal of Dermatologic Surgery and Oncology, 7 (6), pp. 501-508; Collins, N., Adding vitamin C to the wound management mix (2004) Advances in Skin & Wound Care, 17 (3), pp. 109-112. , 2-s2.0-3142628477; Bercea, M., Darie, R.N., Nita, L.E., Morariu, S., Temperature responsive gels based on Pluronic F127 and poly(vinyl alcohol) (2011) Industrial and Engineering Chemistry Research, 50 (7), pp. 4199-4206. , 2-s2.0-79953293749 10.1021/ie1024408; Schmolka, I.R., Artificial skin. I. Preparation and properties of pluronic F-127 gels for treatment of burns. (1972) Journal of Biomedical Materials Research, 6 (6), pp. 571-582. , 2-s2.0-0015420938; Bromberg, L.E., Ron, E.S., Temperature-responsive gels and thermogelling polymer matrices for protein and peptide delivery (1998) Advanced Drug Delivery Reviews, 31 (3), pp. 197-221. , DOI 10.1016/S0169-409X(97)00121-X, PII S0169409X9700121X; Sharma, P.K., Reilly, M.J., Jones, D.N., Robinson, P.M., Bhatia, S.R., The effect of pharmaceuticals on the nanoscale structure of PEO-PPO-PEO micelles (2008) Colloids and Surfaces B: Biointerfaces, 61 (1), pp. 53-60. , DOI 10.1016/j.colsurfb.2007.07.002, PII S0927776507002822; Chang, J.J., Lin, P.J., Lee, Y.H., Yang, M.C., Chien, C.T., The effect of covalent immobilization of sialic acid on the removal of lipopolysaccharide and reactive oxygen species for polyethylene terephthalate (2011) Polymer for Advanced Technologies, 22, pp. 1872-1878; Baoyong, L., Jian, Z., Denglong, C., Min, L., Evaluation of a new type of wound dressing made from recombinant spider silk protein using rat models (2010) Burns, 36 (6), pp. 891-896. , 2-s2.0-77955588339 10.1016/j.burns.2009.12.001; Yates, C.C., Whaley, D., Babu, R., Zhang, J., Krishna, P., Beckman, E., Pasculle, A.W., Wells, A., The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse skin wound models (2007) Biomaterials, 28 (27), pp. 3977-3986. , DOI 10.1016/j.biomaterials.2007.05.008, PII S0142961207004048; Nemes, Z., Steinert, P.M., Bricks and mortar of the epidermal barrier (1999) Experimental and Molecular Medicine, 31 (1), pp. 5-19; Candi, E., Schmidt, R., Melino, G., The cornified envelope: A model of cell death in the skin (2005) Nature Reviews Molecular Cell Biology, 6 (4), pp. 328-340. , DOI 10.1038/nrm1619; Yamaoka, T., Takahashi, Y., Fujisato, T., Lee, C.W., Tsuji, T., Ohta, T., Murakami, A., Kimura, Y., Novel adhesion prevention membrane based on a bioresorbable copoly(ester-ether) comprised of poly-l-lactide and pluronic®: In vitro and in vivo evaluations (2001) Journal of Biomedical Materials Research, 54 (4), pp. 470-479. , DOI 10.1002/1097-4636(20010315)54:4<470::AID-JBM20>3.0.CO;2-X; Oh, S.H., Kim, J.K., Song, K.S., Noh, S.M., Ghil, S.H., Yuk, S.H., Lee, J.H., Prevention of postsurgical tissue adhesion by anti-inflammatory drug-loaded Pluronic mixtures with sol-gel transition behavior (2005) Journal of Biomedical Materials Research - Part A, 72 (3), pp. 306-316. , DOI 10.1002/jbm.a.30239; Hokett, S.D., Cuenin, M.F., O'Neal, R.B., Brennan, W.A., Strong, S.L., Runner, R.R., McPherson, J.C., Van Dyke, T.E., Pluronic polyol effects on human gingival fibroblast attachment and growth (2000) Journal of Periodontology, 71 (5), pp. 803-809; Fowler, E.B., Cuenin, M.F., Hokett, S.D., Peacock, M.E., McPherson III, J.C., Dirksen, T.R., Sharawy, M., Billman, M.A., Evaluation of pluronic polyols as carriers for grafting materials: Study in rat calvaria defects (2002) Journal of Periodontology, 73 (2), pp. 191-197. , DOI 10.1902/jop.2002.73.2.191; Khalil, E.A., Afifi, F.U., Al-Hussaini, M., Evaluation of the wound healing effect of some Jordanian traditional medicinal plants formulated in Pluronic F127 using mice (Mus musculus) (2007) Journal of Ethnopharmacology, 109 (1), pp. 104-112. , DOI 10.1016/j.jep.2006.07.010, PII S0378874106003473; Abd-El-Aleem, S.A., Ferguson, M.W.J., Appleton, I., Kairsingh, S., Jude, E.B., Jones, K., McCollum, C.N., Ireland, G.W., Expression of nitric oxide synthase isoforms and arginase in normal human skin and chronic venous leg ulcers (2000) Journal of Pathology, 191 (4), pp. 434-442; Rojkind, M., Dominguez-Rosales, J.-A., Nieto, N., Greenwel, P., Role of hydrogen peroxide and oxidative stress in healing responses (2002) Cellular and Molecular Life Sciences, 59 (11), pp. 1872-1891. , DOI 10.1007/PL00012511; Moseley, R., Hilton, J.R., Waddington, R.J., Harding, K.G., Stephens, P., Thomas, D.W., Comparison of oxidative stress biomarker profiles between acute and chronic wound environments (2004) Wound Repair and Regeneration, 12 (4), pp. 419-429. , DOI 10.1111/j.1067-1927.2004.12406.x; Rasik, A.M., Shukla, A., Antioxidant status in delayed healing type of wounds (2000) International Journal of Experimental Pathology, 81 (4), pp. 257-263. , 2-s2.0-0033864199 10.1046/j.1365-2613.2000.00158.x; Gopinath, D., Ahmed, M.R., Gomathi, K., Chitra, K., Sehgal, P.K., Jayakumar, R., Dermal wound healing processes with curcumin incorporated collagen films (2004) Biomaterials, 25 (10), pp. 1911-1917. , DOI 10.1016/S0142-9612(03)00625-2; Khanna, S., Roy, S., Bagchi, D., Bagchi, M., Sen, C.K., Upregulation of oxidant-induced VEGF expression in cultured keratinocytes by a grape seed proanthocyanidin extract (2001) Free Radical Biology and Medicine, 31 (1), pp. 38-42. , DOI 10.1016/S0891-5849(01)00544-5, PII S0891584901005445; Fadini, G.P., Albiero, M., Menegazzo, L., Boscaro, E., Pagnin, E., Iori, E., Cosma, C., Avogaro, A., The redox enzyme p66Shc contributes to diabetes and ischemia-induced delay in cutaneous wound healing (2010) Diabetes, 59 (9), pp. 2306-2314. , 2-s2.0-77956379343 10.2337/db09-1727; Al-Mashat, H.A., Kandru, S., Liu, R., Behl, Y., Desta, T., Graves, D.T., Diabetes enhances mRNA levels of proapoptotic genes and caspase activity, which contribute to impaired healing (2006) Diabetes, 55 (2), pp. 487-495. , http://diabetes.diabetesjournals.org/cgi/reprint/55/2/487, DOI 10.2337/diabetes.55.02.06.db05-1201

PY - 2012

Y1 - 2012

N2 - We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer. © 2012 Yen-Hsien Lee et al.

AB - We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer. © 2012 Yen-Hsien Lee et al.

KW - ascorbic acid

KW - collagen

KW - poloxamer

KW - sodium chloride

KW - antioxidant

KW - hydroxyproline

KW - reactive oxygen metabolite

KW - streptozocin

KW - animal cell

KW - animal experiment

KW - animal model

KW - animal tissue

KW - antioxidant activity

KW - apoptosis

KW - article

KW - collagen synthesis

KW - controlled study

KW - dermis

KW - drug delivery system

KW - drug effect

KW - epidermis

KW - female

KW - in vitro study

KW - nonhuman

KW - priority journal

KW - protein content

KW - rat

KW - skin injury

KW - streptozocin diabetes

KW - tissue regeneration

KW - wound contraction

KW - wound healing

KW - animal

KW - chemistry

KW - experimental diabetes mellitus

KW - immunohistochemistry

KW - metabolism

KW - methodology

KW - pathology

KW - pathophysiology

KW - phase transition

KW - skin

KW - time

KW - Wistar rat

KW - Animals

KW - Antioxidants

KW - Apoptosis

KW - Ascorbic Acid

KW - Diabetes Mellitus, Experimental

KW - Drug Delivery Systems

KW - Female

KW - Hydroxyproline

KW - Immunohistochemistry

KW - Phase Transition

KW - Poloxamer

KW - Rats

KW - Rats, Wistar

KW - Reactive Oxygen Species

KW - Skin

KW - Streptozocin

KW - Time Factors

KW - Wound Healing

U2 - 10.1155/2012/504693

DO - 10.1155/2012/504693

M3 - Article

VL - 2012

JO - Experimental Diabesity Research

JF - Experimental Diabesity Research

SN - 1543-8600

ER -