Antioxidant peptides with angiotensin converting enzyme inhibitory activities and applications for angiotensin converting enzyme purification

Wen Chi Hou, Hsien Jung Chen, Yaw Huei Lin

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Five commercial peptides, namely, reduced glutathione (GSH), oxidized glutathione (GSSG), carnosine, homocarnosine, and anserine, were used to test angiotensin converting enzyme inhibitory (ACEI) activities using N-[3-(2-furyl)acryloyl]-Phe-Gly-Gly (FAPGG) as a substrate. All of these peptides showed dose-dependent ACEI activities. Using 50% inhibition (IC50) of captopril as 0.00781 μM for the reference, the IC50 values of GSH, carnosine, homocarnosine, and anserine were determined to be 32.4 μM, 5.216 mM, 6.147 mM, and 6.967 mM, respectively. GSH or carnosine showed mixed noncompetitive inhibition against ACE, When 0.0164 mM GSH or 0.4098 mM carnosine was added, the apparent inhibition constant (Ki) was 49.7 μM or 3.899 mM, respectively. Commercial glutathione-Sepharose 4 fast flow, GSH-coupled CNBr-activated and GSH-coupled EAH-activated Sepharose gels were used for ACE purification. Commercial ACE could be adsorbed only by EAH-coupled GSH gels and eluted off the gels by increasing salt concentrations. These EAH-coupled GSH gels might be developed as affinity aids for ACE purification.

Original languageEnglish
Pages (from-to)1706-1709
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number6
DOIs
Publication statusPublished - Mar 12 2003

Fingerprint

Carnosine
carnosine
peptidyl-dipeptidase A
Enzyme activity
Peptidyl-Dipeptidase A
homocarnosine
Purification
Anserine
Antioxidants
Gels
anserine
gels
peptides
antioxidants
glutathione
Peptides
Glutathione Disulfide
phenylalanyl-glycyl-glycine
Sepharose
agarose

Keywords

  • Angiotensin converting enzyme (ACE)
  • EAH-activated gel
  • Glutathione
  • N-[3-(2-furyl)acryloyl]-Phe-Gly-Gly (FAPGG)
  • Peptide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Antioxidant peptides with angiotensin converting enzyme inhibitory activities and applications for angiotensin converting enzyme purification. / Hou, Wen Chi; Chen, Hsien Jung; Lin, Yaw Huei.

In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 6, 12.03.2003, p. 1706-1709.

Research output: Contribution to journalArticle

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