Antioxidant N-acetylcysteine blocks nerve growth factor-induced H 2O2/ERK signaling in PC12 cells

Liang-Yo Yang, Wun Chang Ko, Chun-Mao Lin, Jia-Wei Lin, Jen Chine Wu, Chien Ju Lin, Huey Hwa Cheng, Chwen-Ming Shih

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We investigated whether H2O2, superoxide, and ERK participate in nerve growth factor (NGF)-induced signaling cascades and whether antioxidant N-acetylcysteine (NAC) regulates these NGF-induced responses. PC12 cells were cultured in medium containing NGF or vehicle with or without NAC pretreatment, and the intracellular H2O2 and superoxide levels and the amount of phosphorylated ERK were evaluated by flow cytometry and Western blotting, respectively. We found that NGF increased intracellular H2O2 concentration and activated ERK but failed to affect intracellular superoxide level. Moreover, NAC counteracted these NGF-induced responses. These findings demonstrate that NAC blocks the NGF-induced H 2O2/ERK signaling in PC12 cells.

Original languageEnglish
Pages (from-to)325-337
Number of pages13
JournalAnnals of the New York Academy of Sciences
Volume1042
DOIs
Publication statusPublished - 2005

Keywords

  • Antioxidant
  • ERK
  • Hydrogen peroxide
  • N-acetylcysteine
  • NGF
  • PC12 cells
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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