Antioxidant N-acetylcysteine blocks nerve growth factor-induced H 2O2/ERK signaling in PC12 cells

Liang-Yo Yang; Wun Chang Ko; Chun-Mao Lin; Jia-Wei Lin; Jen Chine Wu; Chien Ju Lin; Huey Hwa Cheng; Chwen-Ming Shih

doi:10.1196/annals.1338.056

Antioxidant N-acetylcysteine blocks nerve growth factor-induced H ₂O₂/ERK signaling in PC12 cells

Liang-Yo Yang, Wun Chang Ko, Chun-Mao Lin, Jia-Wei Lin, Jen Chine Wu, Chien Ju Lin, Huey Hwa Cheng, Chwen-Ming Shih

Research output: Contribution to journal › Article

11 Citations (Scopus)

Abstract

We investigated whether H₂O₂, superoxide, and ERK participate in nerve growth factor (NGF)-induced signaling cascades and whether antioxidant N-acetylcysteine (NAC) regulates these NGF-induced responses. PC12 cells were cultured in medium containing NGF or vehicle with or without NAC pretreatment, and the intracellular H₂O₂ and superoxide levels and the amount of phosphorylated ERK were evaluated by flow cytometry and Western blotting, respectively. We found that NGF increased intracellular H₂O₂ concentration and activated ERK but failed to affect intracellular superoxide level. Moreover, NAC counteracted these NGF-induced responses. These findings demonstrate that NAC blocks the NGF-induced H ₂O₂/ERK signaling in PC12 cells.

Original language	English
Pages (from-to)	325-337
Number of pages	13
Journal	Annals of the New York Academy of Sciences
Volume	1042
DOIs	https://doi.org/10.1196/annals.1338.056
Publication status	Published - 2005

Keywords

Antioxidant
ERK
Hydrogen peroxide
N-acetylcysteine
NGF
PC12 cells
Reactive oxygen species

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
History and Philosophy of Science

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Yang, L-Y., Ko, W. C., Lin, C-M., Lin, J-W., Wu, J. C., Lin, C. J., Cheng, H. H., & Shih, C-M. (2005). Antioxidant N-acetylcysteine blocks nerve growth factor-induced H ₂O₂/ERK signaling in PC12 cells. Annals of the New York Academy of Sciences, 1042, 325-337. https://doi.org/10.1196/annals.1338.056

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abstract = "We investigated whether H2O2, superoxide, and ERK participate in nerve growth factor (NGF)-induced signaling cascades and whether antioxidant N-acetylcysteine (NAC) regulates these NGF-induced responses. PC12 cells were cultured in medium containing NGF or vehicle with or without NAC pretreatment, and the intracellular H2O2 and superoxide levels and the amount of phosphorylated ERK were evaluated by flow cytometry and Western blotting, respectively. We found that NGF increased intracellular H2O2 concentration and activated ERK but failed to affect intracellular superoxide level. Moreover, NAC counteracted these NGF-induced responses. These findings demonstrate that NAC blocks the NGF-induced H 2O2/ERK signaling in PC12 cells.",

keywords = "Antioxidant, ERK, Hydrogen peroxide, N-acetylcysteine, NGF, PC12 cells, Reactive oxygen species",

author = "Liang-Yo Yang and Ko, {Wun Chang} and Chun-Mao Lin and Jia-Wei Lin and Wu, {Jen Chine} and Lin, {Chien Ju} and Cheng, {Huey Hwa} and Chwen-Ming Shih",

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AU - Yang, Liang-Yo

AU - Ko, Wun Chang

AU - Lin, Chun-Mao

AU - Lin, Jia-Wei

AU - Wu, Jen Chine

AU - Lin, Chien Ju

AU - Cheng, Huey Hwa

AU - Shih, Chwen-Ming

PY - 2005

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KW - Hydrogen peroxide

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KW - NGF

KW - PC12 cells

KW - Reactive oxygen species

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