Abstract
We investigated whether H2O2, superoxide, and ERK participate in nerve growth factor (NGF)-induced signaling cascades and whether antioxidant N-acetylcysteine (NAC) regulates these NGF-induced responses. PC12 cells were cultured in medium containing NGF or vehicle with or without NAC pretreatment, and the intracellular H2O2 and superoxide levels and the amount of phosphorylated ERK were evaluated by flow cytometry and Western blotting, respectively. We found that NGF increased intracellular H2O2 concentration and activated ERK but failed to affect intracellular superoxide level. Moreover, NAC counteracted these NGF-induced responses. These findings demonstrate that NAC blocks the NGF-induced H 2O2/ERK signaling in PC12 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 325-337 |
| Number of pages | 13 |
| Journal | Annals of the New York Academy of Sciences |
| Volume | 1042 |
| DOIs | |
| Publication status | Published - 2005 |
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Keywords
- Antioxidant
- ERK
- Hydrogen peroxide
- N-acetylcysteine
- NGF
- PC12 cells
- Reactive oxygen species
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- History and Philosophy of Science
Cite this
Antioxidant N-acetylcysteine blocks nerve growth factor-induced H 2O2/ERK signaling in PC12 cells. / Yang, Liang-Yo; Ko, Wun Chang; Lin, Chun-Mao; Lin, Jia-Wei; Wu, Jen Chine; Lin, Chien Ju; Cheng, Huey Hwa; Shih, Chwen-Ming.
In: Annals of the New York Academy of Sciences, Vol. 1042, 2005, p. 325-337.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antioxidant N-acetylcysteine blocks nerve growth factor-induced H 2O2/ERK signaling in PC12 cells
AU - Yang, Liang-Yo
AU - Ko, Wun Chang
AU - Lin, Chun-Mao
AU - Lin, Jia-Wei
AU - Wu, Jen Chine
AU - Lin, Chien Ju
AU - Cheng, Huey Hwa
AU - Shih, Chwen-Ming
PY - 2005
Y1 - 2005
N2 - We investigated whether H2O2, superoxide, and ERK participate in nerve growth factor (NGF)-induced signaling cascades and whether antioxidant N-acetylcysteine (NAC) regulates these NGF-induced responses. PC12 cells were cultured in medium containing NGF or vehicle with or without NAC pretreatment, and the intracellular H2O2 and superoxide levels and the amount of phosphorylated ERK were evaluated by flow cytometry and Western blotting, respectively. We found that NGF increased intracellular H2O2 concentration and activated ERK but failed to affect intracellular superoxide level. Moreover, NAC counteracted these NGF-induced responses. These findings demonstrate that NAC blocks the NGF-induced H 2O2/ERK signaling in PC12 cells.
AB - We investigated whether H2O2, superoxide, and ERK participate in nerve growth factor (NGF)-induced signaling cascades and whether antioxidant N-acetylcysteine (NAC) regulates these NGF-induced responses. PC12 cells were cultured in medium containing NGF or vehicle with or without NAC pretreatment, and the intracellular H2O2 and superoxide levels and the amount of phosphorylated ERK were evaluated by flow cytometry and Western blotting, respectively. We found that NGF increased intracellular H2O2 concentration and activated ERK but failed to affect intracellular superoxide level. Moreover, NAC counteracted these NGF-induced responses. These findings demonstrate that NAC blocks the NGF-induced H 2O2/ERK signaling in PC12 cells.
KW - Antioxidant
KW - ERK
KW - Hydrogen peroxide
KW - N-acetylcysteine
KW - NGF
KW - PC12 cells
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=22044452586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22044452586&partnerID=8YFLogxK
U2 - 10.1196/annals.1338.056
DO - 10.1196/annals.1338.056
M3 - Article
C2 - 15965078
AN - SCOPUS:22044452586
VL - 1042
SP - 325
EP - 337
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
SN - 0077-8923
ER -