Antioxidant effects of ginsenoside Re in cardiomyocytes

Jing Tian Xie, Zuo Hui Shao, Terry L. Vanden Hoek, Wei Tien Chang, Jing Li, Sangeeta Mehendale, Chong Zhi Wang, Chin Wang Hsu, Lance B. Becker, Jun Jie Yin, Chun Su Yuan

Research output: Contribution to journalArticle

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Abstract

We have previously demonstrated that American ginseng berry extract exhibited significant protection against oxidant-mediated injury in cardiomyocytes. To extend this work, we sought to investigate the antioxidant effects of Re, a protopanaxatriols-type and single chemical integrant present in American ginseng berry extract, using the same chick cardiomyocyte model of oxidant injury as well as ESR spectroscopy in a cell-free chemical system. In cells exposed to 2 h of H2O2 (0.5 mM), pretreatment with Re (0.05, 0.1, or 0.5 mg/ml for 2 h) significantly attenuated 2′,7′-dichlorofluorescein (DCF) fluorescence by 51% (from 1345 ± 67 to 658 ± 46 a.u., P <0.001), and remarkably reduced cell death (from 51.5 ± 3.0% to 11.8 ± 1.5%, P <0.001, compared to the control). Similar results were also observed in cells exposed to antimycin A (100 μM), a mitochondrial electron transport chain site III inhibitor which increases endogenous oxidative stress. In the ESR study, however, Re failed to reduce the formation of the superoxide/DMPO adduct and DPPH radicals. These results suggest that ginsenoside Re functions as an antioxidant, protecting cardiomyocytes from oxidant injury induced by both exogenous and endogenous oxidants, and that its protective effects may be mostly attributed to scavenging H2O2 and hydroxyl radicals.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalEuropean Journal of Pharmacology
Volume532
Issue number3
DOIs
Publication statusPublished - Feb 27 2006
Externally publishedYes

Fingerprint

Cardiac Myocytes
Oxidants
Antioxidants
Panax
Fruit
Wounds and Injuries
Antimycin A
Cell-Free System
Electron Transport
Superoxides
Hydroxyl Radical
Spectrum Analysis
Oxidative Stress
Cell Death
Fluorescence
ginsenoside Re

Keywords

  • Antimycin A
  • Antioxidant effect
  • Cardiomyocyte
  • ESR spectroscopy
  • Ginsenoside Re
  • HO
  • Ischemia and reperfusion damage

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Xie, J. T., Shao, Z. H., Vanden Hoek, T. L., Chang, W. T., Li, J., Mehendale, S., ... Yuan, C. S. (2006). Antioxidant effects of ginsenoside Re in cardiomyocytes. European Journal of Pharmacology, 532(3), 201-207. https://doi.org/10.1016/j.ejphar.2006.01.001

Antioxidant effects of ginsenoside Re in cardiomyocytes. / Xie, Jing Tian; Shao, Zuo Hui; Vanden Hoek, Terry L.; Chang, Wei Tien; Li, Jing; Mehendale, Sangeeta; Wang, Chong Zhi; Hsu, Chin Wang; Becker, Lance B.; Yin, Jun Jie; Yuan, Chun Su.

In: European Journal of Pharmacology, Vol. 532, No. 3, 27.02.2006, p. 201-207.

Research output: Contribution to journalArticle

Xie, JT, Shao, ZH, Vanden Hoek, TL, Chang, WT, Li, J, Mehendale, S, Wang, CZ, Hsu, CW, Becker, LB, Yin, JJ & Yuan, CS 2006, 'Antioxidant effects of ginsenoside Re in cardiomyocytes', European Journal of Pharmacology, vol. 532, no. 3, pp. 201-207. https://doi.org/10.1016/j.ejphar.2006.01.001
Xie JT, Shao ZH, Vanden Hoek TL, Chang WT, Li J, Mehendale S et al. Antioxidant effects of ginsenoside Re in cardiomyocytes. European Journal of Pharmacology. 2006 Feb 27;532(3):201-207. https://doi.org/10.1016/j.ejphar.2006.01.001
Xie, Jing Tian ; Shao, Zuo Hui ; Vanden Hoek, Terry L. ; Chang, Wei Tien ; Li, Jing ; Mehendale, Sangeeta ; Wang, Chong Zhi ; Hsu, Chin Wang ; Becker, Lance B. ; Yin, Jun Jie ; Yuan, Chun Su. / Antioxidant effects of ginsenoside Re in cardiomyocytes. In: European Journal of Pharmacology. 2006 ; Vol. 532, No. 3. pp. 201-207.
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AU - Mehendale, Sangeeta

AU - Wang, Chong Zhi

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