Antioxidant effect of hydralazine on retinal pigment epithelial cells and its potential use in the therapy of age-related macular degeneration

Yu Wen Cheng, George C Y Chiou

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

• Aim: To investigate the antioxidant effect of hydralazine under hypoxia-induced damage on retinal pigment epithelial (ARPE-19) cells and the role of reactive oxygen species (ROS) in this effect. • Methods: Human retinal pigment epithelial (hRPE) cells were used to investigate the effect of hydralazine on oxidative stress, including tert-butyl hydroxyperoxide (t-BHP), H2 O2, sodium azide (NaN3), and hypoxia induced cell damage. Cell viability was determined by MTT assay. • Results: When ARPE-19 cells were treated with oxidative stress induced by ROS, hydralazine showed concentration-dependent protection against t-BHP, H2 O2 and hypoxia induced cell damage but not NaN3. Nitric oxide (NO) was not involved in this effect. • Conclusion: Hydralazine showed antioxidant potential against oxidative stress induced damage in ARPE-19 cells. These effects might be caused through scavenger of ROS. Thus, hydralazine could be used for the treatment of age-related macular degeneration (AMD).

Original languageEnglish
Pages (from-to)1059-1064
Number of pages6
JournalInternational Journal of Ophthalmology
Volume8
Issue number6
Publication statusPublished - Jun 25 2008
Externally publishedYes

Fingerprint

Hydralazine
Retinal Pigments
Macular Degeneration
Antioxidants
Epithelial Cells
Sodium Azide
Cell Hypoxia
Reactive Oxygen Species
Oxidative Stress
Therapeutics
Cell Survival
Nitric Oxide

Keywords

  • Age-related macular degeneration (AMD)
  • Reactive oxygen species (ROS)
  • Tea-butyl hydroxyperoxide (t-BHP)

ASJC Scopus subject areas

  • Ophthalmology

Cite this

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abstract = "• Aim: To investigate the antioxidant effect of hydralazine under hypoxia-induced damage on retinal pigment epithelial (ARPE-19) cells and the role of reactive oxygen species (ROS) in this effect. • Methods: Human retinal pigment epithelial (hRPE) cells were used to investigate the effect of hydralazine on oxidative stress, including tert-butyl hydroxyperoxide (t-BHP), H2 O2, sodium azide (NaN3), and hypoxia induced cell damage. Cell viability was determined by MTT assay. • Results: When ARPE-19 cells were treated with oxidative stress induced by ROS, hydralazine showed concentration-dependent protection against t-BHP, H2 O2 and hypoxia induced cell damage but not NaN3. Nitric oxide (NO) was not involved in this effect. • Conclusion: Hydralazine showed antioxidant potential against oxidative stress induced damage in ARPE-19 cells. These effects might be caused through scavenger of ROS. Thus, hydralazine could be used for the treatment of age-related macular degeneration (AMD).",
keywords = "Age-related macular degeneration (AMD), Reactive oxygen species (ROS), Tea-butyl hydroxyperoxide (t-BHP), Age-related macular degeneration (AMD), Reactive oxygen species (ROS), Tea-butyl hydroxyperoxide (t-BHP)",
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T1 - Antioxidant effect of hydralazine on retinal pigment epithelial cells and its potential use in the therapy of age-related macular degeneration

AU - Cheng, Yu Wen

AU - Chiou, George C Y

PY - 2008/6/25

Y1 - 2008/6/25

N2 - • Aim: To investigate the antioxidant effect of hydralazine under hypoxia-induced damage on retinal pigment epithelial (ARPE-19) cells and the role of reactive oxygen species (ROS) in this effect. • Methods: Human retinal pigment epithelial (hRPE) cells were used to investigate the effect of hydralazine on oxidative stress, including tert-butyl hydroxyperoxide (t-BHP), H2 O2, sodium azide (NaN3), and hypoxia induced cell damage. Cell viability was determined by MTT assay. • Results: When ARPE-19 cells were treated with oxidative stress induced by ROS, hydralazine showed concentration-dependent protection against t-BHP, H2 O2 and hypoxia induced cell damage but not NaN3. Nitric oxide (NO) was not involved in this effect. • Conclusion: Hydralazine showed antioxidant potential against oxidative stress induced damage in ARPE-19 cells. These effects might be caused through scavenger of ROS. Thus, hydralazine could be used for the treatment of age-related macular degeneration (AMD).

AB - • Aim: To investigate the antioxidant effect of hydralazine under hypoxia-induced damage on retinal pigment epithelial (ARPE-19) cells and the role of reactive oxygen species (ROS) in this effect. • Methods: Human retinal pigment epithelial (hRPE) cells were used to investigate the effect of hydralazine on oxidative stress, including tert-butyl hydroxyperoxide (t-BHP), H2 O2, sodium azide (NaN3), and hypoxia induced cell damage. Cell viability was determined by MTT assay. • Results: When ARPE-19 cells were treated with oxidative stress induced by ROS, hydralazine showed concentration-dependent protection against t-BHP, H2 O2 and hypoxia induced cell damage but not NaN3. Nitric oxide (NO) was not involved in this effect. • Conclusion: Hydralazine showed antioxidant potential against oxidative stress induced damage in ARPE-19 cells. These effects might be caused through scavenger of ROS. Thus, hydralazine could be used for the treatment of age-related macular degeneration (AMD).

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KW - Tea-butyl hydroxyperoxide (t-BHP)

KW - Age-related macular degeneration (AMD)

KW - Reactive oxygen species (ROS)

KW - Tea-butyl hydroxyperoxide (t-BHP)

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