Antioxidant, antinociceptive, and anti-inflammatory activities from actinidia callosa var. callosa in vitro and in vivo

Jung Chun Liao, Jeng Shyan Deng, Ying Chih Lin, Chao Ying Lee, Min Min Lee, Wen Chi Hou, Shyh Shyun Huang, Guan Jhong Huang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Actinidia callosa var. callosa has been widely used to treat antipyretic, analgesic, anti-inflammation, abdominal pain, and fever in Taiwan. The aim of this study was to evaluate the antioxidant, antinociceptive, and anti-inflammatory lipopolysaccharide-(LPS-)induced nitric oxide (NO) production in RAW264.7 macrophages and pawedema induced by -carrageenan activities of the methanol extract from A. callosa. In HPLC analysis, the fingerprint chromatogram of ethyl-acetate fraction of A. callosa (EAAC) was established. EAAC showed the highest TEAC and DPPH radical scavenging activities, respectively. We evaluated that EAAC and the reference compound of catechin and caffeic acid decreased the LPS-induced NO production in RAW264.7 cells. Treatment of male ICR mice with EAAC significantly inhibited the numbers of acetic acid-induced writhing response and the formalin-induced pain in the late phase. Administration of EAAC showed a concentration-dependent inhibition on paw edema development after Carr treatment in mice. Anti-inflammatory mechanisms of EAAC might be correlated to the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and heme oxygenase-1 (HO-1) in vitro and in vivo. Overall, the results showed that EAAC demonstrated antioxidant, antinociceptive, and anti-inflammatory activity, which supports previous claims of the traditional use for inflammation and pain.

Original languageEnglish
Article number129152
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012

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Actinidia
Anti-Inflammatory Agents
Antioxidants
Nitric Oxide
Inflammation
Pain
Antipyretics
Inbred ICR Mouse
Heme Oxygenase-1
Catechin
Carrageenan
Dermatoglyphics
Nitric Oxide Synthase Type II
Cyclooxygenase 2
In Vitro Techniques
ethyl acetate
Taiwan
Acetic Acid
Abdominal Pain
Formaldehyde

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Antioxidant, antinociceptive, and anti-inflammatory activities from actinidia callosa var. callosa in vitro and in vivo. / Liao, Jung Chun; Deng, Jeng Shyan; Lin, Ying Chih; Lee, Chao Ying; Lee, Min Min; Hou, Wen Chi; Huang, Shyh Shyun; Huang, Guan Jhong.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 129152, 2012.

Research output: Contribution to journalArticle

Liao, Jung Chun ; Deng, Jeng Shyan ; Lin, Ying Chih ; Lee, Chao Ying ; Lee, Min Min ; Hou, Wen Chi ; Huang, Shyh Shyun ; Huang, Guan Jhong. / Antioxidant, antinociceptive, and anti-inflammatory activities from actinidia callosa var. callosa in vitro and in vivo. In: Evidence-based Complementary and Alternative Medicine. 2012 ; Vol. 2012.
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