Abstract

The wrinkle-fruited leaf flower (Phyllanthus urinaria L.) (Euphorbiaceae) is widely used as a traditional folk medicine for inflammatory relief. Geraniin, the hydrolysable tannin, was purified by a series of chromatographic processes from the 70% aqueous acetone extracts of P. urinaria and identified by NMR [1H (500 MHz) and 13C NMR (126 MHz)] spectra and mass spectroscopy. The scavenging activities of geraniin against DPPH radicals (half-inhibition concentration, IC50, were 0.92 and 1.27 μM, respectively, for pH 4.5 and pH 7.9), hydroxyl radicals (IC50 was 0.11 μM by deoxyribose method and 1.44 μM by electron spin resonance method), and superoxide radicals (IC50 were 2.65 μM) were determined in comparison with positive controls. The inhibitory activities against xanthine oxidase (IC50 were 30.49 μM) were measured. Geraniin also showed dose-dependent inhibitory activities against semicarbazide-sensitive amine oxidase (SSAO, IC50 were 6.58 μM) and against angiotensin converting enzyme (ACE, IC50 were 13.22 μM). For kinetic property determinations, geraniin showed competitive inhibitions against SSAO (the apparent inhibition constant, Ki, was 0.70 μM) and mixed noncompetitive inhibitions against ACE. Spontaneously hypertensive rats (SHR, 10-week age) were orally administered to once (5 mg geraniin/kg SHR), and changes of systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured over 24 h and compared with the positive control of captopril (2 mg/kg SHR). The geraniin showed antihypertensive activity in lowering SBP and DBP and showed a significant difference from the blank (distilled water) at 2, 4, 6, 8, and 24 h. Healthy food products could use geraniin for antioxidant protection and therapeutic effects in the future.

Original languageEnglish
Pages (from-to)2485-2492
Number of pages8
JournalFood and Chemical Toxicology
Volume46
Issue number7
DOIs
Publication statusPublished - Jul 2008

Fingerprint

Phyllanthus urinaria
Phyllanthus
Amine Oxidase (Copper-Containing)
antihypertensive effect
amines
Antihypertensive Agents
inhibitory concentration 50
Antioxidants
Inhibitory Concentration 50
Blood Pressure
antioxidants
Blood pressure
diastolic blood pressure
systolic blood pressure
traditional medicine
Traditional Medicine
Nuclear magnetic resonance
Hydrolyzable Tannins
xanthine oxidase
Deoxyribose

Keywords

  • Angiotensin converting enzyme (ACE)
  • Antihypertensive activity
  • Antioxidant
  • Geraniin
  • Phyllanthus urinaria
  • Semicarbazide-sensitive amine oxidase (SSAO)

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

@article{8388d280dc534e4c981b268b36b1c591,
title = "Antioxidant, anti-semicarbazide-sensitive amine oxidase, and anti-hypertensive activities of geraniin isolated from Phyllanthus urinaria",
abstract = "The wrinkle-fruited leaf flower (Phyllanthus urinaria L.) (Euphorbiaceae) is widely used as a traditional folk medicine for inflammatory relief. Geraniin, the hydrolysable tannin, was purified by a series of chromatographic processes from the 70{\%} aqueous acetone extracts of P. urinaria and identified by NMR [1H (500 MHz) and 13C NMR (126 MHz)] spectra and mass spectroscopy. The scavenging activities of geraniin against DPPH radicals (half-inhibition concentration, IC50, were 0.92 and 1.27 μM, respectively, for pH 4.5 and pH 7.9), hydroxyl radicals (IC50 was 0.11 μM by deoxyribose method and 1.44 μM by electron spin resonance method), and superoxide radicals (IC50 were 2.65 μM) were determined in comparison with positive controls. The inhibitory activities against xanthine oxidase (IC50 were 30.49 μM) were measured. Geraniin also showed dose-dependent inhibitory activities against semicarbazide-sensitive amine oxidase (SSAO, IC50 were 6.58 μM) and against angiotensin converting enzyme (ACE, IC50 were 13.22 μM). For kinetic property determinations, geraniin showed competitive inhibitions against SSAO (the apparent inhibition constant, Ki, was 0.70 μM) and mixed noncompetitive inhibitions against ACE. Spontaneously hypertensive rats (SHR, 10-week age) were orally administered to once (5 mg geraniin/kg SHR), and changes of systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured over 24 h and compared with the positive control of captopril (2 mg/kg SHR). The geraniin showed antihypertensive activity in lowering SBP and DBP and showed a significant difference from the blank (distilled water) at 2, 4, 6, 8, and 24 h. Healthy food products could use geraniin for antioxidant protection and therapeutic effects in the future.",
keywords = "Angiotensin converting enzyme (ACE), Antihypertensive activity, Antioxidant, Geraniin, Phyllanthus urinaria, Semicarbazide-sensitive amine oxidase (SSAO)",
author = "Lin, {Shyr Yi} and Wang, {Ching Chiung} and Lu, {Yeh Lin} and Wu, {Wen Chun} and Hou, {Wen Chi}",
year = "2008",
month = "7",
doi = "10.1016/j.fct.2008.04.007",
language = "English",
volume = "46",
pages = "2485--2492",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Elsevier Limited",
number = "7",

}

TY - JOUR

T1 - Antioxidant, anti-semicarbazide-sensitive amine oxidase, and anti-hypertensive activities of geraniin isolated from Phyllanthus urinaria

AU - Lin, Shyr Yi

AU - Wang, Ching Chiung

AU - Lu, Yeh Lin

AU - Wu, Wen Chun

AU - Hou, Wen Chi

PY - 2008/7

Y1 - 2008/7

N2 - The wrinkle-fruited leaf flower (Phyllanthus urinaria L.) (Euphorbiaceae) is widely used as a traditional folk medicine for inflammatory relief. Geraniin, the hydrolysable tannin, was purified by a series of chromatographic processes from the 70% aqueous acetone extracts of P. urinaria and identified by NMR [1H (500 MHz) and 13C NMR (126 MHz)] spectra and mass spectroscopy. The scavenging activities of geraniin against DPPH radicals (half-inhibition concentration, IC50, were 0.92 and 1.27 μM, respectively, for pH 4.5 and pH 7.9), hydroxyl radicals (IC50 was 0.11 μM by deoxyribose method and 1.44 μM by electron spin resonance method), and superoxide radicals (IC50 were 2.65 μM) were determined in comparison with positive controls. The inhibitory activities against xanthine oxidase (IC50 were 30.49 μM) were measured. Geraniin also showed dose-dependent inhibitory activities against semicarbazide-sensitive amine oxidase (SSAO, IC50 were 6.58 μM) and against angiotensin converting enzyme (ACE, IC50 were 13.22 μM). For kinetic property determinations, geraniin showed competitive inhibitions against SSAO (the apparent inhibition constant, Ki, was 0.70 μM) and mixed noncompetitive inhibitions against ACE. Spontaneously hypertensive rats (SHR, 10-week age) were orally administered to once (5 mg geraniin/kg SHR), and changes of systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured over 24 h and compared with the positive control of captopril (2 mg/kg SHR). The geraniin showed antihypertensive activity in lowering SBP and DBP and showed a significant difference from the blank (distilled water) at 2, 4, 6, 8, and 24 h. Healthy food products could use geraniin for antioxidant protection and therapeutic effects in the future.

AB - The wrinkle-fruited leaf flower (Phyllanthus urinaria L.) (Euphorbiaceae) is widely used as a traditional folk medicine for inflammatory relief. Geraniin, the hydrolysable tannin, was purified by a series of chromatographic processes from the 70% aqueous acetone extracts of P. urinaria and identified by NMR [1H (500 MHz) and 13C NMR (126 MHz)] spectra and mass spectroscopy. The scavenging activities of geraniin against DPPH radicals (half-inhibition concentration, IC50, were 0.92 and 1.27 μM, respectively, for pH 4.5 and pH 7.9), hydroxyl radicals (IC50 was 0.11 μM by deoxyribose method and 1.44 μM by electron spin resonance method), and superoxide radicals (IC50 were 2.65 μM) were determined in comparison with positive controls. The inhibitory activities against xanthine oxidase (IC50 were 30.49 μM) were measured. Geraniin also showed dose-dependent inhibitory activities against semicarbazide-sensitive amine oxidase (SSAO, IC50 were 6.58 μM) and against angiotensin converting enzyme (ACE, IC50 were 13.22 μM). For kinetic property determinations, geraniin showed competitive inhibitions against SSAO (the apparent inhibition constant, Ki, was 0.70 μM) and mixed noncompetitive inhibitions against ACE. Spontaneously hypertensive rats (SHR, 10-week age) were orally administered to once (5 mg geraniin/kg SHR), and changes of systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured over 24 h and compared with the positive control of captopril (2 mg/kg SHR). The geraniin showed antihypertensive activity in lowering SBP and DBP and showed a significant difference from the blank (distilled water) at 2, 4, 6, 8, and 24 h. Healthy food products could use geraniin for antioxidant protection and therapeutic effects in the future.

KW - Angiotensin converting enzyme (ACE)

KW - Antihypertensive activity

KW - Antioxidant

KW - Geraniin

KW - Phyllanthus urinaria

KW - Semicarbazide-sensitive amine oxidase (SSAO)

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JO - Food and Chemical Toxicology

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