Antioxidant and semicarbazide-sensitive amine oxidase inhibitory activities of glucuronic acid hydroxamate

Yuh Hwa Liu, Wen Li Liang, Chi Ching Lee, Yow Fu Tsai, Wen Chi Hou

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The antioxidant and inhibitory activities of glucuronic acid (GluA) and its synthesized 1-methoxyl-6-glucuronic acid hydroxamate (GluA-NHOH) against semicarbazide-sensitive amine oxidase (SSAO) were investigated. The scavenging activities of GluA-NHOH against 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radicals (half-inhibition concentration [IC50], 152 μM) and hydroxyl radicals (IC50, 70 nM) were determined. The protective activities of GluA-NHOH against hydroxyl radical-mediated calf thymus DNA damage and peroxynitrite-mediated dihydrorhodamine oxidation were also investigated. GluA-NHOH, but not GluA, exhibited dose-dependent protective activities. GluA-NHOH, but not GluA, effectively inhibited bovine SSAO activities in a dose-dependent manner, and was further confirmed by AO activity stains on an acrylamide gel. GluA-NHOH showed mixed noncompetitive inhibition of bovine SSAO with respect to benzylamine (substrate) and benzylamine-SSAO (substrate-enzyme complex). The Vmax′ and Km′ values were reduced in the presence of GluA-NHOH (0.04 mM).

Original languageEnglish
Pages (from-to)423-428
Number of pages6
JournalFood Chemistry
Volume129
Issue number2
DOIs
Publication statusPublished - Nov 15 2011

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Amine Oxidase (Copper-Containing)
Glucuronic Acid
glucuronic acid
amines
Antioxidants
antioxidants
hydroxyl radicals
Hydroxyl Radical
Inhibitory Concentration 50
inhibitory concentration 50
Peroxynitrous Acid
Acrylamide
enzyme substrates
acrylamides
cattle
Scavenging
Substrates
dosage
Hydrates
DNA damage

Keywords

  • 1-Methoxyl-6-glucuronic acid hydroxamate (GluA-NHOH)
  • Antioxidant activity
  • Electron spin resonance (ESR)

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Antioxidant and semicarbazide-sensitive amine oxidase inhibitory activities of glucuronic acid hydroxamate. / Liu, Yuh Hwa; Liang, Wen Li; Lee, Chi Ching; Tsai, Yow Fu; Hou, Wen Chi.

In: Food Chemistry, Vol. 129, No. 2, 15.11.2011, p. 423-428.

Research output: Contribution to journalArticle

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