Antioxidant and semicarbazide-sensitive amine oxidase inhibitory activities of glucuronic acid hydroxamate

Yuh Hwa Liu, Wen Li Liang, Chi Ching Lee, Yow Fu Tsai, Wen Chi Hou

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The antioxidant and inhibitory activities of glucuronic acid (GluA) and its synthesized 1-methoxyl-6-glucuronic acid hydroxamate (GluA-NHOH) against semicarbazide-sensitive amine oxidase (SSAO) were investigated. The scavenging activities of GluA-NHOH against 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radicals (half-inhibition concentration [IC50], 152 μM) and hydroxyl radicals (IC50, 70 nM) were determined. The protective activities of GluA-NHOH against hydroxyl radical-mediated calf thymus DNA damage and peroxynitrite-mediated dihydrorhodamine oxidation were also investigated. GluA-NHOH, but not GluA, exhibited dose-dependent protective activities. GluA-NHOH, but not GluA, effectively inhibited bovine SSAO activities in a dose-dependent manner, and was further confirmed by AO activity stains on an acrylamide gel. GluA-NHOH showed mixed noncompetitive inhibition of bovine SSAO with respect to benzylamine (substrate) and benzylamine-SSAO (substrate-enzyme complex). The Vmax′ and Km′ values were reduced in the presence of GluA-NHOH (0.04 mM).

Original languageEnglish
Pages (from-to)423-428
Number of pages6
JournalFood Chemistry
Volume129
Issue number2
DOIs
Publication statusPublished - Nov 15 2011

Keywords

  • 1-Methoxyl-6-glucuronic acid hydroxamate (GluA-NHOH)
  • Antioxidant activity
  • Electron spin resonance (ESR)

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

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