Antioxidant and antiglycation activities of the synthesised dipeptide, Asn-Trp, derived from computer-aided simulation of yam dioscorin hydrolysis and its analogue, Gln-Trp

Chuan Hsiao Han, Yin Shiou Lin, Shyr Yi Lin, Wen Chi Hou

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Previous studies have shown that the Trp residue contributes to a high oxygen radical absorbance capacity (ORAC). Therefore, in this study, a Trp-containing dipeptide derived from a computer-aided simulation of pepsin hydrolysis of the yam tuber, dioscorin - namely, Asn-Trp (NW), and its analogue, Gln-Trp (QW) - were synthesised to compare their antioxidant and antiglycation activities with carnosine, homocarnosine, or glutathione (GSH). The antioxidant assays included hydroxyl radical-scavenging activity, anti-AAPH-induced hemolysis, and ORAC activity. NW had a significantly higher antioxidant activity than had QW and performed much better than carnosine, homocarnosine, or GSH. Using bovine serum albumin (BSA)/galactose or BSA/glucose as experimental models, NW had better antiglycation effects than had QW, as detected by an anti-Nε-(carboxymethyl)lysine (anti-CML) antibody. Moreover, NW and QW (50-200 μM) showed protection against methylglyoxal-induced cell deaths in human umbilical vein endothelial cells. These results suggest that NW, derived from computer-aided simulation of dioscorin hydrolysis, exhibits antioxidant and antiglycation activities, which thus shows the benefits of the yam tuber as an antioxidant-rich food.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalFood Chemistry
Volume147
DOIs
Publication statusPublished - 2014

Keywords

  • Antioxidant activity
  • Asn-Trp
  • Hemolysis
  • N-(carboxymethyl) lysine (CML)
  • Oxygen radical absorbance capacity (ORAC)
  • Yam tuber dioscorin

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

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