Antioxidant and anti-inflammatory properties of Dichondra repens Forst. and its reference compounds

Ming Jyh Sheu, Jeng Shyan Deng, Ming Hsing Huang, Jung Chun Liao, Chieh Hsi Wu, Shyh Shyun Huang, Guan Jhong Huang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Dichondra repens (DR) is the main constituent in herbal beverages and consumed daily as a nutrition supplement for the liver in Taiwan. This study investigated the antioxidant and anti-inflammatory effects of D. repens ethanol extract (EDR) and its reference compounds ex vivo and in vivo. Fingerprint chromatograms (from HPLC) indicated that EDR contained vanillin, umbelliferone and scopoletin. EDR was evaluated for its antioxidant effects and LPS-induced NO production in RAW 264.7 cells. EDR decreased the LPS-induced NO production and expressions of iNOS and COX-2 in RAW 264.7 cells. In vivo anti-inflammatory activities of EDR were assessed in mouse paw oedema, induced by λ-carrageenan (Carr). We investigate the antioxidant mechanism of EDR via studies of the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the liver and the levels of malondialdehyde (MDA) and nitrite oxide (NO) in the oedematous paw. Serum NO and TNF-α were also measured. EDR exerts anti-inflammatory effects by suppressing TNF-α, NO, and might be related to the decrement of the level of MDA in the oedema paw via increasing the activities of CAT, SOD and GPx in the liver. The results show that EDR might be a natural antioxidant and anti-inflammatory agent.

Original languageEnglish
Pages (from-to)1010-1018
Number of pages9
JournalFood Chemistry
Volume132
Issue number2
DOIs
Publication statusPublished - May 15 2012
Externally publishedYes

Fingerprint

Dichondra
Nitrites
anti-inflammatory activity
nitrites
Oxides
oxides
Anti-Inflammatory Agents
Antioxidants
antioxidants
Liver
7-hydroxycoumarin
Glutathione Peroxidase
Malondialdehyde
glutathione peroxidase
Catalase
edema
malondialdehyde
liver
Superoxide Dismutase
Edema

Keywords

  • Anti-inflammation
  • Antioxidant
  • Dichondra repens
  • MDA
  • NO
  • TNF-α

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Antioxidant and anti-inflammatory properties of Dichondra repens Forst. and its reference compounds. / Sheu, Ming Jyh; Deng, Jeng Shyan; Huang, Ming Hsing; Liao, Jung Chun; Wu, Chieh Hsi; Huang, Shyh Shyun; Huang, Guan Jhong.

In: Food Chemistry, Vol. 132, No. 2, 15.05.2012, p. 1010-1018.

Research output: Contribution to journalArticle

Sheu, Ming Jyh ; Deng, Jeng Shyan ; Huang, Ming Hsing ; Liao, Jung Chun ; Wu, Chieh Hsi ; Huang, Shyh Shyun ; Huang, Guan Jhong. / Antioxidant and anti-inflammatory properties of Dichondra repens Forst. and its reference compounds. In: Food Chemistry. 2012 ; Vol. 132, No. 2. pp. 1010-1018.
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