Antioxidant and anti-inflammatory properties of Cardiospermum halicacabum and its reference compounds ex vivo and in vivo

Ming Hsing Huang, Shyh Shyun Huang, Bor Sen Wang, Chieh Hsi Wu, Ming Jyh Sheu, Wen Chi Hou, Shiang Shiou Lin, Guan Jhong Huang

Research output: Contribution to journalArticle

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Abstract

Aims of the study: Cardiospermum halicacabum (CH) has been used in Chinese medicine for a long time. However, its fingerprint chromatogram, antioxidant, anti-inflammatory effects and mechanism are still needed to be explored. Therefore, the aims of this study investigated the antioxidant and anti-inflammatory effects of CH extracts and its reference compounds ex vivo and in vivo. Materials and methods: In HPLC analysis, the fingerprint chromatogram of ethanolic extract of CH (ECH) was established. The effects of ACH (aqueous extract of CH) and ECH extracts were assessed for the antioxidant and LPS-induced NO production in RAW264.7 cells. In vivo anti-inflammatory activities of ECH were evaluated in mouse paw edema induced by λ-carrageenan (Carr). We investigate the anti-inflammatory mechanism of ECH via studies of the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver and the levels of malondialdehyde (MDA) and nitrite oxide (NO) in the edema paw. Serum NO and TNF-α were also measured. Results: ECH had better antioxidant activity than that of ACH. In the anti-inflammatory test, ECH inhibited the development of paw edema induced by Carr and increased the activities of CAT, SOD and GPx in the liver tissue. ECH also decreased the level of NO in edematous paw tissue and in serum level, and diminished the level of serum TNF-α at the fifth hour after Carr injection. Conclusions: ECH exerts anti-inflammatory effects by suppressing TNF-α and NO. The anti-inflammatory mechanism of ECH might be related to the decrement of the level of MDA in the edema paw via increasing the activities of CAT, SOD and GPx in the liver. The results showed that ECH might serve as a natural antioxidant and anti-inflammatory agent.

Original languageEnglish
Pages (from-to)743-750
Number of pages8
JournalJournal of Ethnopharmacology
Volume133
Issue number2
DOIs
Publication statusPublished - Jan 27 2011

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Sapindaceae
Anti-Inflammatory Agents
Antioxidants
Nitrites
Oxides
Carrageenan
Edema
Glutathione Peroxidase
Catalase
Superoxide Dismutase
Dermatoglyphics
Malondialdehyde
Liver
Serum
High Pressure Liquid Chromatography
Medicine

Keywords

  • Anti-inflammation
  • Antioxidant
  • Cardiospermum halicacabum
  • Chinese medicine
  • NO
  • TNF-α

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Antioxidant and anti-inflammatory properties of Cardiospermum halicacabum and its reference compounds ex vivo and in vivo. / Huang, Ming Hsing; Huang, Shyh Shyun; Wang, Bor Sen; Wu, Chieh Hsi; Sheu, Ming Jyh; Hou, Wen Chi; Lin, Shiang Shiou; Huang, Guan Jhong.

In: Journal of Ethnopharmacology, Vol. 133, No. 2, 27.01.2011, p. 743-750.

Research output: Contribution to journalArticle

Huang, Ming Hsing ; Huang, Shyh Shyun ; Wang, Bor Sen ; Wu, Chieh Hsi ; Sheu, Ming Jyh ; Hou, Wen Chi ; Lin, Shiang Shiou ; Huang, Guan Jhong. / Antioxidant and anti-inflammatory properties of Cardiospermum halicacabum and its reference compounds ex vivo and in vivo. In: Journal of Ethnopharmacology. 2011 ; Vol. 133, No. 2. pp. 743-750.
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AU - Wu, Chieh Hsi

AU - Sheu, Ming Jyh

AU - Hou, Wen Chi

AU - Lin, Shiang Shiou

AU - Huang, Guan Jhong

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