Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima

Shyh Shyun Huang, Chuan Sung Chiu, Tsung Hui Lin, Min Min Lee, Chao Ying Lee, Shu Jen Chang, Wen Chi Hou, Guan Jhong Huang, Jeng Shyan Deng

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Ethnopharmacological relevance Centipeda minima (L.) is traditionally used in Chinese folk medicine for the treatments of rhinitis, sinusitis, relieving pain, reducing swelling, and treating cancer for a long history in Taiwan. However, there is no scientific evidence which supports the use in the literature. Aim of the study The aim of this study was to evaluate the antioxidant and anti-inflammatory activities of the aqueous extract of Centipeda minima (ACM). Materials and methods The following activities were investigated: antioxidant activities [2,2′-azino-bis (3-ethylbenzothiazoline-6- sulphonic acid) (ABTS), DPPH (1,1-diphenyl-2-picrylhydrazyl)], and anti-inflammatory [lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW264.7 macrophages and paw-edema induced by λ-carrageenan (Carr)]. We also investigated the anti-inflammatory mechanism of ACM via studies of the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and the levels of malondialdehyde (MDA) in the edema paw. Serum NO, tumor necrosis factor α (TNF-α), and interleukin-1β (IL-1β) were also measured in vivo. In HPLC analysis, the fingerprint chromatogram of ACM was established. Results ACM showed the highest TEAC and DPPH radical scavenging activities, respectively. ACM also had highest contents of polyphenol and flavonoid contents. We evaluated that ACM and the reference compound of protocatechualdehyde and caffeic acid decreased the LPS-induced NO production in RAW264.7 cells. Administration of ACM showed a concentration dependent inhibition on paw edema development after Carr treatment in mice. The anti-inflammatory effects of ACM could be via NO, TNF-α, and IL-1β suppressions and associated with the increase in the activities of antioxidant enzymes. Western blotting revealed that ACM decreased Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions. Conclusions Anti-inflammatory mechanisms of ACM might be correlated to the decrease in the level of Malondialdehyde (MDA), iNOS, and COX-2 via increasing the activities of CAT, SOD, and GPx in the edema paw. Overall, the results showed that ACM demonstrated antioxidant and anti-inflammatory activity, which supports previous claims of the traditional use for inflammation and pain.

Original languageEnglish
Pages (from-to)395-405
Number of pages11
JournalJournal of Ethnopharmacology
Volume147
Issue number2
DOIs
Publication statusPublished - May 20 2013

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Anti-Inflammatory Agents
Antioxidants
Carrageenan
Edema
Nitric Oxide
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Glutathione Peroxidase
Malondialdehyde
Interleukin-1
Catalase
Superoxide Dismutase
Lipopolysaccharides
Tumor Necrosis Factor-alpha
Pain
Sulfonic Acids
Sinusitis
Dermatoglyphics
Polyphenols
Traditional Medicine

Keywords

  • Anti-inflammatory activity
  • Antioxidant activity
  • Centipeda minima
  • Chinese medicine

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Huang, S. S., Chiu, C. S., Lin, T. H., Lee, M. M., Lee, C. Y., Chang, S. J., ... Deng, J. S. (2013). Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima. Journal of Ethnopharmacology, 147(2), 395-405. https://doi.org/10.1016/j.jep.2013.03.025

Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima. / Huang, Shyh Shyun; Chiu, Chuan Sung; Lin, Tsung Hui; Lee, Min Min; Lee, Chao Ying; Chang, Shu Jen; Hou, Wen Chi; Huang, Guan Jhong; Deng, Jeng Shyan.

In: Journal of Ethnopharmacology, Vol. 147, No. 2, 20.05.2013, p. 395-405.

Research output: Contribution to journalArticle

Huang, SS, Chiu, CS, Lin, TH, Lee, MM, Lee, CY, Chang, SJ, Hou, WC, Huang, GJ & Deng, JS 2013, 'Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima', Journal of Ethnopharmacology, vol. 147, no. 2, pp. 395-405. https://doi.org/10.1016/j.jep.2013.03.025
Huang, Shyh Shyun ; Chiu, Chuan Sung ; Lin, Tsung Hui ; Lee, Min Min ; Lee, Chao Ying ; Chang, Shu Jen ; Hou, Wen Chi ; Huang, Guan Jhong ; Deng, Jeng Shyan. / Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima. In: Journal of Ethnopharmacology. 2013 ; Vol. 147, No. 2. pp. 395-405.
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abstract = "Ethnopharmacological relevance Centipeda minima (L.) is traditionally used in Chinese folk medicine for the treatments of rhinitis, sinusitis, relieving pain, reducing swelling, and treating cancer for a long history in Taiwan. However, there is no scientific evidence which supports the use in the literature. Aim of the study The aim of this study was to evaluate the antioxidant and anti-inflammatory activities of the aqueous extract of Centipeda minima (ACM). Materials and methods The following activities were investigated: antioxidant activities [2,2′-azino-bis (3-ethylbenzothiazoline-6- sulphonic acid) (ABTS), DPPH (1,1-diphenyl-2-picrylhydrazyl)], and anti-inflammatory [lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW264.7 macrophages and paw-edema induced by λ-carrageenan (Carr)]. We also investigated the anti-inflammatory mechanism of ACM via studies of the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and the levels of malondialdehyde (MDA) in the edema paw. Serum NO, tumor necrosis factor α (TNF-α), and interleukin-1β (IL-1β) were also measured in vivo. In HPLC analysis, the fingerprint chromatogram of ACM was established. Results ACM showed the highest TEAC and DPPH radical scavenging activities, respectively. ACM also had highest contents of polyphenol and flavonoid contents. We evaluated that ACM and the reference compound of protocatechualdehyde and caffeic acid decreased the LPS-induced NO production in RAW264.7 cells. Administration of ACM showed a concentration dependent inhibition on paw edema development after Carr treatment in mice. The anti-inflammatory effects of ACM could be via NO, TNF-α, and IL-1β suppressions and associated with the increase in the activities of antioxidant enzymes. Western blotting revealed that ACM decreased Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions. Conclusions Anti-inflammatory mechanisms of ACM might be correlated to the decrease in the level of Malondialdehyde (MDA), iNOS, and COX-2 via increasing the activities of CAT, SOD, and GPx in the edema paw. Overall, the results showed that ACM demonstrated antioxidant and anti-inflammatory activity, which supports previous claims of the traditional use for inflammation and pain.",
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AU - Hou, Wen Chi

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AU - Deng, Jeng Shyan

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