Antioxidant activities of trypsin inhibitor, a 33 KDa root storage protein of sweet potato (Ipomoea batatas (L.) Lam cv. Tainong 57)

W. C. Hou, Y. C. Chen, H. J. Chen, Y. H. Lin, Ling-Ling Yang, M. H. Lee

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Trypsin inhibitors (TIs), root storage proteins, were purified from sweet potato (Ipomoea batatas [L.] Lam cv. Tainong 57) roots by trypsin affinity column according to the methods of Hou and Lin (Plant Sci. 1997, 126, 11-19 and Plant Sci. 1997, 128, 151-158), A single band of 33 kDa TI was obtained by preparative sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS - PAGE) gels. This purified 33 kDa TI had scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. There was positive correlation between scavenging effects against DPPH (2 to 22%) and amounts of 33 kDa TI (1.92 to 46 pmol). The scavenging activities of 33 kDa TI against DPPH were calculated from linear regression to be about one-third of those of glutathione between 5 and 80 pmol. Using electron paramagnetic resonance (EPR) spectrometry for hydroxyl radical detection, it was found that 33 kDa TI could capture hydroxyl radical, and the intensities of EPR signal were significantly decreased from 1.5 to 6 pmol of 33 kDa TI compared to those of the controls. It is suggested that 33 kDa TI, one of the sweet potato root storage proteins, may play a role as an antioxidant in roots and may be beneficial to health when it is consumed.

Original languageEnglish
Pages (from-to)2978-2981
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume49
Issue number6
Publication statusPublished - 2001

Fingerprint

Ipomoea batatas
Trypsin Inhibitors
trypsin inhibitors
storage proteins
sweet potatoes
Antioxidants
antioxidant activity
Scavenging
Proteins
electron paramagnetic resonance spectroscopy
Electron Spin Resonance Spectroscopy
hydroxyl radicals
Hydroxyl Radical
biphenyl
Paramagnetic resonance
Electrophoresis
Linear regression
Sodium Dodecyl Sulfate
Trypsin
trypsin

Keywords

  • Antioxidant
  • Electron paramagnetic resonance
  • Hydroxyl radical
  • Sweet potato
  • Trypsin inhibitor

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

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title = "Antioxidant activities of trypsin inhibitor, a 33 KDa root storage protein of sweet potato (Ipomoea batatas (L.) Lam cv. Tainong 57)",
abstract = "Trypsin inhibitors (TIs), root storage proteins, were purified from sweet potato (Ipomoea batatas [L.] Lam cv. Tainong 57) roots by trypsin affinity column according to the methods of Hou and Lin (Plant Sci. 1997, 126, 11-19 and Plant Sci. 1997, 128, 151-158), A single band of 33 kDa TI was obtained by preparative sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS - PAGE) gels. This purified 33 kDa TI had scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. There was positive correlation between scavenging effects against DPPH (2 to 22{\%}) and amounts of 33 kDa TI (1.92 to 46 pmol). The scavenging activities of 33 kDa TI against DPPH were calculated from linear regression to be about one-third of those of glutathione between 5 and 80 pmol. Using electron paramagnetic resonance (EPR) spectrometry for hydroxyl radical detection, it was found that 33 kDa TI could capture hydroxyl radical, and the intensities of EPR signal were significantly decreased from 1.5 to 6 pmol of 33 kDa TI compared to those of the controls. It is suggested that 33 kDa TI, one of the sweet potato root storage proteins, may play a role as an antioxidant in roots and may be beneficial to health when it is consumed.",
keywords = "Antioxidant, Electron paramagnetic resonance, Hydroxyl radical, Sweet potato, Trypsin inhibitor",
author = "Hou, {W. C.} and Chen, {Y. C.} and Chen, {H. J.} and Lin, {Y. H.} and Ling-Ling Yang and Lee, {M. H.}",
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language = "English",
volume = "49",
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journal = "Journal of Agricultural and Food Chemistry",
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TY - JOUR

T1 - Antioxidant activities of trypsin inhibitor, a 33 KDa root storage protein of sweet potato (Ipomoea batatas (L.) Lam cv. Tainong 57)

AU - Hou, W. C.

AU - Chen, Y. C.

AU - Chen, H. J.

AU - Lin, Y. H.

AU - Yang, Ling-Ling

AU - Lee, M. H.

PY - 2001

Y1 - 2001

N2 - Trypsin inhibitors (TIs), root storage proteins, were purified from sweet potato (Ipomoea batatas [L.] Lam cv. Tainong 57) roots by trypsin affinity column according to the methods of Hou and Lin (Plant Sci. 1997, 126, 11-19 and Plant Sci. 1997, 128, 151-158), A single band of 33 kDa TI was obtained by preparative sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS - PAGE) gels. This purified 33 kDa TI had scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. There was positive correlation between scavenging effects against DPPH (2 to 22%) and amounts of 33 kDa TI (1.92 to 46 pmol). The scavenging activities of 33 kDa TI against DPPH were calculated from linear regression to be about one-third of those of glutathione between 5 and 80 pmol. Using electron paramagnetic resonance (EPR) spectrometry for hydroxyl radical detection, it was found that 33 kDa TI could capture hydroxyl radical, and the intensities of EPR signal were significantly decreased from 1.5 to 6 pmol of 33 kDa TI compared to those of the controls. It is suggested that 33 kDa TI, one of the sweet potato root storage proteins, may play a role as an antioxidant in roots and may be beneficial to health when it is consumed.

AB - Trypsin inhibitors (TIs), root storage proteins, were purified from sweet potato (Ipomoea batatas [L.] Lam cv. Tainong 57) roots by trypsin affinity column according to the methods of Hou and Lin (Plant Sci. 1997, 126, 11-19 and Plant Sci. 1997, 128, 151-158), A single band of 33 kDa TI was obtained by preparative sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS - PAGE) gels. This purified 33 kDa TI had scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. There was positive correlation between scavenging effects against DPPH (2 to 22%) and amounts of 33 kDa TI (1.92 to 46 pmol). The scavenging activities of 33 kDa TI against DPPH were calculated from linear regression to be about one-third of those of glutathione between 5 and 80 pmol. Using electron paramagnetic resonance (EPR) spectrometry for hydroxyl radical detection, it was found that 33 kDa TI could capture hydroxyl radical, and the intensities of EPR signal were significantly decreased from 1.5 to 6 pmol of 33 kDa TI compared to those of the controls. It is suggested that 33 kDa TI, one of the sweet potato root storage proteins, may play a role as an antioxidant in roots and may be beneficial to health when it is consumed.

KW - Antioxidant

KW - Electron paramagnetic resonance

KW - Hydroxyl radical

KW - Sweet potato

KW - Trypsin inhibitor

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