Antioxidant activities of the synthesized thiol-contained peptides derived from computer-aided pepsin hydrolysis of yam tuber storage protein, dioscorin

Chuan Hsiao Han, Ju Chi Liu, Sheng Uei Fang, Wen Chi Hou

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Our previous report showed that yam dioscorin and its peptic hydrolysates exhibit radical scavenging activities; however, the functions of these peptic hydrolases are still under investigation. In this study, the thiol-containing peptides derived from computer-aided simulation of pepsin hydrolysis of dioscorin, namely, KTCGNGME (diotide1), PPCSE (diotide2), CDDRVIRTPLT (diotide3), KTCGY (diotide4), and PPCTE (diotide5) were synthesized to compare their antioxidant activities with GSH and/or carnosine by examining hydroxyl radical scavenging activity by electron spin resonance spectrometry, anti-low-density lipoprotein peroxidation, anti-AAPH-induced hemolysis, and oxygen radical absorbance capacity activity. We found that while all the synthesized diotides showed antioxidant activity, diotide4 exhibited the highest levels. Moreover, all diotides (100 μM) showed protective effects against methylglyoxal-induced human umbilical vein endothelial cell death. These results suggest that thiol-containing diotides derived from dioscorin hydrolysis exhibit antioxidant activities and reveal the benefits of yam tuber as an antioxidant-rich food.

Original languageEnglish
Pages (from-to)923-930
Number of pages8
JournalFood Chemistry
Volume138
Issue number2-3
DOIs
Publication statusPublished - Jun 1 2013

Fingerprint

Dioscorea
yams
Pepsin A
pepsin
storage proteins
thiols
Sulfhydryl Compounds
Hydrolysis
tubers
Antioxidants
antioxidant activity
hydrolysis
peptides
Peptides
Scavenging
digestion
carnosine
Digestion
oxygen radical absorbance capacity
Proteins

Keywords

  • Antioxidant activity
  • Hemolysis
  • Low-density lipoprotein
  • Oxygen radical absorbance capacity
  • Yam tuber

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Antioxidant activities of the synthesized thiol-contained peptides derived from computer-aided pepsin hydrolysis of yam tuber storage protein, dioscorin. / Han, Chuan Hsiao; Liu, Ju Chi; Fang, Sheng Uei; Hou, Wen Chi.

In: Food Chemistry, Vol. 138, No. 2-3, 01.06.2013, p. 923-930.

Research output: Contribution to journalArticle

@article{e747d09bc5f8448b8ee6ac662b230ece,
title = "Antioxidant activities of the synthesized thiol-contained peptides derived from computer-aided pepsin hydrolysis of yam tuber storage protein, dioscorin",
abstract = "Our previous report showed that yam dioscorin and its peptic hydrolysates exhibit radical scavenging activities; however, the functions of these peptic hydrolases are still under investigation. In this study, the thiol-containing peptides derived from computer-aided simulation of pepsin hydrolysis of dioscorin, namely, KTCGNGME (diotide1), PPCSE (diotide2), CDDRVIRTPLT (diotide3), KTCGY (diotide4), and PPCTE (diotide5) were synthesized to compare their antioxidant activities with GSH and/or carnosine by examining hydroxyl radical scavenging activity by electron spin resonance spectrometry, anti-low-density lipoprotein peroxidation, anti-AAPH-induced hemolysis, and oxygen radical absorbance capacity activity. We found that while all the synthesized diotides showed antioxidant activity, diotide4 exhibited the highest levels. Moreover, all diotides (100 μM) showed protective effects against methylglyoxal-induced human umbilical vein endothelial cell death. These results suggest that thiol-containing diotides derived from dioscorin hydrolysis exhibit antioxidant activities and reveal the benefits of yam tuber as an antioxidant-rich food.",
keywords = "Antioxidant activity, Hemolysis, Low-density lipoprotein, Oxygen radical absorbance capacity, Yam tuber",
author = "Han, {Chuan Hsiao} and Liu, {Ju Chi} and Fang, {Sheng Uei} and Hou, {Wen Chi}",
year = "2013",
month = "6",
day = "1",
doi = "10.1016/j.foodchem.2012.11.101",
language = "English",
volume = "138",
pages = "923--930",
journal = "Food Chemistry",
issn = "0308-8146",
publisher = "Elsevier Ltd",
number = "2-3",

}

TY - JOUR

T1 - Antioxidant activities of the synthesized thiol-contained peptides derived from computer-aided pepsin hydrolysis of yam tuber storage protein, dioscorin

AU - Han, Chuan Hsiao

AU - Liu, Ju Chi

AU - Fang, Sheng Uei

AU - Hou, Wen Chi

PY - 2013/6/1

Y1 - 2013/6/1

N2 - Our previous report showed that yam dioscorin and its peptic hydrolysates exhibit radical scavenging activities; however, the functions of these peptic hydrolases are still under investigation. In this study, the thiol-containing peptides derived from computer-aided simulation of pepsin hydrolysis of dioscorin, namely, KTCGNGME (diotide1), PPCSE (diotide2), CDDRVIRTPLT (diotide3), KTCGY (diotide4), and PPCTE (diotide5) were synthesized to compare their antioxidant activities with GSH and/or carnosine by examining hydroxyl radical scavenging activity by electron spin resonance spectrometry, anti-low-density lipoprotein peroxidation, anti-AAPH-induced hemolysis, and oxygen radical absorbance capacity activity. We found that while all the synthesized diotides showed antioxidant activity, diotide4 exhibited the highest levels. Moreover, all diotides (100 μM) showed protective effects against methylglyoxal-induced human umbilical vein endothelial cell death. These results suggest that thiol-containing diotides derived from dioscorin hydrolysis exhibit antioxidant activities and reveal the benefits of yam tuber as an antioxidant-rich food.

AB - Our previous report showed that yam dioscorin and its peptic hydrolysates exhibit radical scavenging activities; however, the functions of these peptic hydrolases are still under investigation. In this study, the thiol-containing peptides derived from computer-aided simulation of pepsin hydrolysis of dioscorin, namely, KTCGNGME (diotide1), PPCSE (diotide2), CDDRVIRTPLT (diotide3), KTCGY (diotide4), and PPCTE (diotide5) were synthesized to compare their antioxidant activities with GSH and/or carnosine by examining hydroxyl radical scavenging activity by electron spin resonance spectrometry, anti-low-density lipoprotein peroxidation, anti-AAPH-induced hemolysis, and oxygen radical absorbance capacity activity. We found that while all the synthesized diotides showed antioxidant activity, diotide4 exhibited the highest levels. Moreover, all diotides (100 μM) showed protective effects against methylglyoxal-induced human umbilical vein endothelial cell death. These results suggest that thiol-containing diotides derived from dioscorin hydrolysis exhibit antioxidant activities and reveal the benefits of yam tuber as an antioxidant-rich food.

KW - Antioxidant activity

KW - Hemolysis

KW - Low-density lipoprotein

KW - Oxygen radical absorbance capacity

KW - Yam tuber

UR - http://www.scopus.com/inward/record.url?scp=84873722842&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873722842&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2012.11.101

DO - 10.1016/j.foodchem.2012.11.101

M3 - Article

C2 - 23411197

AN - SCOPUS:84873722842

VL - 138

SP - 923

EP - 930

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

IS - 2-3

ER -