Antioxidant activities of mucilages from different Taiwanese yam cultivars

Shyr Yi Lin, Hao Yu Liu, Yeh Lin Lu, Wen Chi Hou

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The antioxident effects of crude mucilages (CM) and partially purified mucilages (PPM) from three different Taiwanese yam cultivars - including Dioscorea alata L. cv. Tainong 1 (TN1), Dioscorea alata L. cv. Tainong 2 (TN2), and D. alata L. var. purpurea (Roxb.) Ming-Jen (MJ) - were evaluated, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and superoxide radical scavenging activities Electron spin resonance (ESR) spectrometry was used to measure hydroxyl radical scavenging activities. The IC50 stands for the concentration required for 50% scavenging activity. The IC 50 of CM and PPM against DPPH radical was 0.329, 0.279; 0.547, 0.653; and 0.847, 0.631 mg/ml, respectively, for TN1, TN2 and MJ. The IC50 of CM and PPM against hydroxyl radical by spectrophotometry was 0.668, 1.146; 1.461, 1.096; and 0.946, 1.554 mg/ml, respectively, for TN1, TN2 and MJ. The IC50 of CM and PPM against superoxide radical was 0.802, 0.368; 0.681, 0.258; and 0.086, 0.148 mg/ml, respectively, for TN1, TN2 and MJ. Using ESR to detect hydroxyl radicals, the IC50 of PPM against hydroxyl radical was 0.083, 0.47, and 0.004 mg/ml, respectively, for TN1, TN2 and MJ. The results demonstrated that different cultivars of yams exhibited different antioxidant ability, and the purification process was able to partially increase the antioxidant activity of the mucilage polysaccharide. Taken together, these results suggest that mucilage polysaccharides of the yam tuber might play an important role on antiradicals and antioxidants.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalBotanical Bulletin of Academia Sinica
Volume46
Issue number3
Publication statusPublished - Jul 2005

Fingerprint

mucilages
yams
antioxidant activity
cultivars
hydroxyl radicals
inhibitory concentration 50
Dioscorea alata
electron paramagnetic resonance spectroscopy
superoxide anion
spectroscopy
polysaccharides
antioxidants
purification methods
tubers

Keywords

  • 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical
  • Electron spin resonance (ESR)
  • Hydroxyl radical
  • Mucilage
  • Superoxide radical
  • Yam

ASJC Scopus subject areas

  • Plant Science

Cite this

Antioxidant activities of mucilages from different Taiwanese yam cultivars. / Lin, Shyr Yi; Liu, Hao Yu; Lu, Yeh Lin; Hou, Wen Chi.

In: Botanical Bulletin of Academia Sinica, Vol. 46, No. 3, 07.2005, p. 183-188.

Research output: Contribution to journalArticle

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abstract = "The antioxident effects of crude mucilages (CM) and partially purified mucilages (PPM) from three different Taiwanese yam cultivars - including Dioscorea alata L. cv. Tainong 1 (TN1), Dioscorea alata L. cv. Tainong 2 (TN2), and D. alata L. var. purpurea (Roxb.) Ming-Jen (MJ) - were evaluated, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and superoxide radical scavenging activities Electron spin resonance (ESR) spectrometry was used to measure hydroxyl radical scavenging activities. The IC50 stands for the concentration required for 50{\%} scavenging activity. The IC 50 of CM and PPM against DPPH radical was 0.329, 0.279; 0.547, 0.653; and 0.847, 0.631 mg/ml, respectively, for TN1, TN2 and MJ. The IC50 of CM and PPM against hydroxyl radical by spectrophotometry was 0.668, 1.146; 1.461, 1.096; and 0.946, 1.554 mg/ml, respectively, for TN1, TN2 and MJ. The IC50 of CM and PPM against superoxide radical was 0.802, 0.368; 0.681, 0.258; and 0.086, 0.148 mg/ml, respectively, for TN1, TN2 and MJ. Using ESR to detect hydroxyl radicals, the IC50 of PPM against hydroxyl radical was 0.083, 0.47, and 0.004 mg/ml, respectively, for TN1, TN2 and MJ. The results demonstrated that different cultivars of yams exhibited different antioxidant ability, and the purification process was able to partially increase the antioxidant activity of the mucilage polysaccharide. Taken together, these results suggest that mucilage polysaccharides of the yam tuber might play an important role on antiradicals and antioxidants.",
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