Antioxidant activities and phytochemical study of leaf extracts from 18 indigenous tree species in Taiwan

Shang Tse Ho, Yu Tang Tung, Yong Long Chen, Ying Ying Zhao, Min Jay Chung, Jyh Horng Wu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The objective of this study is to assess antioxidant activities of methanolic extracts from the leaves of 18 indigenous tree species in Taiwan. Results revealed that, among 18 species, Acer oliverianum exhibited the best free radical scavenging activities. The IC50 values were 5.8 and 11.8μg/mL on DPPH radical and superoxide radical scavenging activities, respectively. In addition, A. oliverianum also exhibited the strongest ferrous ion chelating activity. Based on a bioactivity-guided isolation principle, the resulting methanolic crude extracts of A. oliverianum leaves were fractionated to yield soluble fractions of hexane, EtOAc, BuOH, and water. Of these, the EtOAc fraction had the best antioxidant activity. Furthermore, 8 specific phytochemicals were isolated and identified from the EtOAc fraction. Among them, 1,2,3,4,6-O-penta-galloyl-β-D-glucopyranose had the best free radical scavenging activity. These results demonstrate that methanolic extracts and their derived phytochemicals of A. oliverianum leaves have excellent antioxidant activities and thus they have great potential as sources for natural health products.

Original languageEnglish
Article number215959
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Phytochemicals
Taiwan
Antioxidants
Free Radicals
Acer
Rubiaceae
Hexanes
Complex Mixtures
Superoxides
Inhibitory Concentration 50
Ions
Water
Health

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Antioxidant activities and phytochemical study of leaf extracts from 18 indigenous tree species in Taiwan. / Ho, Shang Tse; Tung, Yu Tang; Chen, Yong Long; Zhao, Ying Ying; Chung, Min Jay; Wu, Jyh Horng.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 215959, 2012.

Research output: Contribution to journalArticle

@article{ec7916d5e80942da8cb7f90617ec4208,
title = "Antioxidant activities and phytochemical study of leaf extracts from 18 indigenous tree species in Taiwan",
abstract = "The objective of this study is to assess antioxidant activities of methanolic extracts from the leaves of 18 indigenous tree species in Taiwan. Results revealed that, among 18 species, Acer oliverianum exhibited the best free radical scavenging activities. The IC50 values were 5.8 and 11.8μg/mL on DPPH radical and superoxide radical scavenging activities, respectively. In addition, A. oliverianum also exhibited the strongest ferrous ion chelating activity. Based on a bioactivity-guided isolation principle, the resulting methanolic crude extracts of A. oliverianum leaves were fractionated to yield soluble fractions of hexane, EtOAc, BuOH, and water. Of these, the EtOAc fraction had the best antioxidant activity. Furthermore, 8 specific phytochemicals were isolated and identified from the EtOAc fraction. Among them, 1,2,3,4,6-O-penta-galloyl-β-D-glucopyranose had the best free radical scavenging activity. These results demonstrate that methanolic extracts and their derived phytochemicals of A. oliverianum leaves have excellent antioxidant activities and thus they have great potential as sources for natural health products.",
author = "Ho, {Shang Tse} and Tung, {Yu Tang} and Chen, {Yong Long} and Zhao, {Ying Ying} and Chung, {Min Jay} and Wu, {Jyh Horng}",
year = "2012",
doi = "10.1155/2012/215959",
language = "English",
volume = "2012",
journal = "Evidence-based Complementary and Alternative Medicine",
issn = "1741-427X",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Antioxidant activities and phytochemical study of leaf extracts from 18 indigenous tree species in Taiwan

AU - Ho, Shang Tse

AU - Tung, Yu Tang

AU - Chen, Yong Long

AU - Zhao, Ying Ying

AU - Chung, Min Jay

AU - Wu, Jyh Horng

PY - 2012

Y1 - 2012

N2 - The objective of this study is to assess antioxidant activities of methanolic extracts from the leaves of 18 indigenous tree species in Taiwan. Results revealed that, among 18 species, Acer oliverianum exhibited the best free radical scavenging activities. The IC50 values were 5.8 and 11.8μg/mL on DPPH radical and superoxide radical scavenging activities, respectively. In addition, A. oliverianum also exhibited the strongest ferrous ion chelating activity. Based on a bioactivity-guided isolation principle, the resulting methanolic crude extracts of A. oliverianum leaves were fractionated to yield soluble fractions of hexane, EtOAc, BuOH, and water. Of these, the EtOAc fraction had the best antioxidant activity. Furthermore, 8 specific phytochemicals were isolated and identified from the EtOAc fraction. Among them, 1,2,3,4,6-O-penta-galloyl-β-D-glucopyranose had the best free radical scavenging activity. These results demonstrate that methanolic extracts and their derived phytochemicals of A. oliverianum leaves have excellent antioxidant activities and thus they have great potential as sources for natural health products.

AB - The objective of this study is to assess antioxidant activities of methanolic extracts from the leaves of 18 indigenous tree species in Taiwan. Results revealed that, among 18 species, Acer oliverianum exhibited the best free radical scavenging activities. The IC50 values were 5.8 and 11.8μg/mL on DPPH radical and superoxide radical scavenging activities, respectively. In addition, A. oliverianum also exhibited the strongest ferrous ion chelating activity. Based on a bioactivity-guided isolation principle, the resulting methanolic crude extracts of A. oliverianum leaves were fractionated to yield soluble fractions of hexane, EtOAc, BuOH, and water. Of these, the EtOAc fraction had the best antioxidant activity. Furthermore, 8 specific phytochemicals were isolated and identified from the EtOAc fraction. Among them, 1,2,3,4,6-O-penta-galloyl-β-D-glucopyranose had the best free radical scavenging activity. These results demonstrate that methanolic extracts and their derived phytochemicals of A. oliverianum leaves have excellent antioxidant activities and thus they have great potential as sources for natural health products.

UR - http://www.scopus.com/inward/record.url?scp=84863291807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863291807&partnerID=8YFLogxK

U2 - 10.1155/2012/215959

DO - 10.1155/2012/215959

M3 - Article

VL - 2012

JO - Evidence-based Complementary and Alternative Medicine

JF - Evidence-based Complementary and Alternative Medicine

SN - 1741-427X

M1 - 215959

ER -