Antiobesity effect of Lactobacillus reuteri 263 associated with energy metabolism remodeling of white adipose tissue in high-energy-diet-fed rats

Li Han Chen, Yi Hsing Chen, Kuan Chen Cheng, Ting Yi Chien, Ching Hung Chan, Shu Ping Tsao, Hui Yu Huang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Obesity is a serious and costly issue to the medical welfare worldwide. Probiotics have been suggested as one of the candidates to resolve the obesity-associated problems, but how they combat obesity is not fully understood. Herein, we investigated the effects of Lactobacillus reuteri 263 (L. reuteri 263) on antiobesity using four groups of Sprague–Dawley rats (n=10/group), namely, C (normal diet with vehicle treatment), HE [high-energy diet (HED) with vehicle treatment], 1X (HED with 2.1×10 9 CFU/kg/day of L. reuteri 263) and 5X (HED with 1.05×10 10 CFU/kg/day of L. reuteri 263), for 8 weeks. L. reuteri 263 improved the phenomenon of obesity, serum levels of proinflammatory factors and antioxidant enzymes. More importantly, L. reuteri 263 increased oxygen consumption in white adipose tissue (WAT). The mRNA expressions of thermogenesis genes uncoupling protein-1, uncoupling protein-3, carnitine palmitoyltransferase-1 and cell death-inducing DFFA-like effector-a were up-regulated in WAT of the 5X group. Moreover, L. reuteri 263 might induce browning of WAT due to the higher mRNA levels of browning-related genes peroxisome proliferator-activated receptor-γ, PR domain containing-16, Pparγ coactivator-1α, bone morphogenetic protein-7 and fibroblast growth factor-21 in the 1X and 5X groups compared to the HE group. Finally, L. reuteri 263 altered the expressions of genes involved in glucose and lipid metabolisms in WAT, including increasing the levels of glucose transporter type 4 and carbohydrate-responsive element-binding protein and decreasing the expression of Acetyl-CoA carboxylase-1. The results suggest that L. reuteri 263 may treat obesity through energy metabolism remodeling of WAT in the high-energy-diet-induced obese rats.

Original languageEnglish
Pages (from-to)87-94
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume54
DOIs
Publication statusPublished - Apr 1 2018
Externally publishedYes

Fingerprint

Lactobacillus reuteri
White Adipose Tissue
Nutrition
Energy Metabolism
Rats
Tissue
Diet
Obesity
Genes
Glucose Transporter Type 4
Bone Morphogenetic Protein 7
Carnitine O-Palmitoyltransferase
Acetyl-CoA Carboxylase
Messenger RNA
Peroxisome Proliferator-Activated Receptors
Cell death
Carrier Proteins
Proteins
Antioxidants
Carbohydrates

Keywords

  • Antiobesity
  • Browning of white adipose tissue
  • Energy metabolism remodeling
  • Lactobacillus reuteri 263
  • Probiotics

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Antiobesity effect of Lactobacillus reuteri 263 associated with energy metabolism remodeling of white adipose tissue in high-energy-diet-fed rats. / Chen, Li Han; Chen, Yi Hsing; Cheng, Kuan Chen; Chien, Ting Yi; Chan, Ching Hung; Tsao, Shu Ping; Huang, Hui Yu.

In: Journal of Nutritional Biochemistry, Vol. 54, 01.04.2018, p. 87-94.

Research output: Contribution to journalArticle

Chen, Li Han ; Chen, Yi Hsing ; Cheng, Kuan Chen ; Chien, Ting Yi ; Chan, Ching Hung ; Tsao, Shu Ping ; Huang, Hui Yu. / Antiobesity effect of Lactobacillus reuteri 263 associated with energy metabolism remodeling of white adipose tissue in high-energy-diet-fed rats. In: Journal of Nutritional Biochemistry. 2018 ; Vol. 54. pp. 87-94.
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