Antiobesity activities of indole-3-carbinol in high-fat-diet-induced obese mice

H. P. Chang, Mei Ling Wang, M. H. Chan, Yen Shuo Chiu, Yue-Hwa Chen

Research output: Contribution to journalArticle

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Abstract

Objective: This study investigated the effects of indole-3-carbinol (I3C), a cruciferous vegetable derivative, on obesity and its associated factors in high-fat-diet-induced obese (DIO) mice. Methods: Eighteen male C57BL/6 mice were randomly assigned to one of three groups: basal, high fat (HF), and HF + 5 mg/kg of I3C intraperitoneally (HFI). After 12 wk of treatment, obesity-associated factors, including body weight, organ weight, serum concentrations of glucose, triacylglycerol, insulin, and adipokines, and macrophage accumulation and lipid metabolism-associated factors in epididymal adipose tissue were measured. Results: Body weight and epididymal adipose tissue weight were greater (P <0.01), and adipocytes were larger in the HF group than in the basal and HFI groups. Compared with the HF group, the HFI group had improved glucose tolerance, a higher serum adiponectin concentration, lower serum glucose, triacylglycerol, insulin, and leptin concentrations, and less F4/80 expression in epididymal adipose tissue (P <0.001). Furthermore, I3C treatment decreased acetyl coenzyme A carboxylase mRNA expression (P <0.05) and increased peroxisome proliferator-activated receptor-γ protein expression (P <0.05) in epididymal adipose tissue of DIO mice. Conclusion: The I3C treatment decreased body weight and fat accumulation and infiltrated macrophages in epididymal adipose tissue of DIO mice, and these reductions were associated with improved glucose tolerance and with modulated expression of adipokines and lipogenic-associated gene products, including acetyl coenzyme A carboxylase and peroxisome proliferator-activated receptor-γ.

Original languageEnglish
Pages (from-to)463-470
Number of pages8
JournalNutrition
Volume27
Issue number4
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Obese Mice
High Fat Diet
Adipose Tissue
Fats
Acetyl-CoA Carboxylase
Glucose
Peroxisome Proliferator-Activated Receptors
Adipokines
Body Weight
Triglycerides
Obesity
Serum
Macrophages
Insulin
Diet
Organ Size
Adiponectin
Leptin
Inbred C57BL Mouse
Lipid Metabolism

Keywords

  • Adipokines
  • Glucose tolerance
  • Indole-3-carbinol
  • Macrophage infiltration
  • Obesity
  • Peroxisome proliferator-activated receptor

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Antiobesity activities of indole-3-carbinol in high-fat-diet-induced obese mice. / Chang, H. P.; Wang, Mei Ling; Chan, M. H.; Chiu, Yen Shuo; Chen, Yue-Hwa.

In: Nutrition, Vol. 27, No. 4, 04.2011, p. 463-470.

Research output: Contribution to journalArticle

Chang, H. P. ; Wang, Mei Ling ; Chan, M. H. ; Chiu, Yen Shuo ; Chen, Yue-Hwa. / Antiobesity activities of indole-3-carbinol in high-fat-diet-induced obese mice. In: Nutrition. 2011 ; Vol. 27, No. 4. pp. 463-470.
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abstract = "Objective: This study investigated the effects of indole-3-carbinol (I3C), a cruciferous vegetable derivative, on obesity and its associated factors in high-fat-diet-induced obese (DIO) mice. Methods: Eighteen male C57BL/6 mice were randomly assigned to one of three groups: basal, high fat (HF), and HF + 5 mg/kg of I3C intraperitoneally (HFI). After 12 wk of treatment, obesity-associated factors, including body weight, organ weight, serum concentrations of glucose, triacylglycerol, insulin, and adipokines, and macrophage accumulation and lipid metabolism-associated factors in epididymal adipose tissue were measured. Results: Body weight and epididymal adipose tissue weight were greater (P <0.01), and adipocytes were larger in the HF group than in the basal and HFI groups. Compared with the HF group, the HFI group had improved glucose tolerance, a higher serum adiponectin concentration, lower serum glucose, triacylglycerol, insulin, and leptin concentrations, and less F4/80 expression in epididymal adipose tissue (P <0.001). Furthermore, I3C treatment decreased acetyl coenzyme A carboxylase mRNA expression (P <0.05) and increased peroxisome proliferator-activated receptor-γ protein expression (P <0.05) in epididymal adipose tissue of DIO mice. Conclusion: The I3C treatment decreased body weight and fat accumulation and infiltrated macrophages in epididymal adipose tissue of DIO mice, and these reductions were associated with improved glucose tolerance and with modulated expression of adipokines and lipogenic-associated gene products, including acetyl coenzyme A carboxylase and peroxisome proliferator-activated receptor-γ.",
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