Antimutagenic constituents of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) with potential cancer chemopreventive activity

Huang Hui Chen, Wenchang Chiang, Jang Yang Chang, Ya Lin Chien, Ching Kuo Lee, Ko Jiunn Liu, Yen Ting Cheng, Ting Fang Chen, Yueh Hsiung Kuo, Ching Chuan Kuo

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Adlay has long been used in traditional Chinese medicine and as a nourishing food. The acetone extract of adlay hull had previously been demonstrated to possess potent antimutagenic activity. The aims of this study were to identify the antimutagenic constituents from adlay hull by using Ames antimutagenic activity-guide isolation procedures and to investigate their chemopreventive efficacies in cultured cells. The results demonstrated that six compounds showing great antimutagenic activity were identified by spectroscopic methods and by comparison with authentic samples to be p-hydroxybenzaldehyde, vanillin, syringaldehyde, trans-coniferylaldehyde, sinapaldehyde, and coixol. Two of them, trans-coniferylaldehyde and sinapaldehyde, exhibit relatively potent scavenging of DPPH radicals, inhibit TPA stimulated superoxide anion generation in neutrophil-like leukocytes, and induce Nrf2/ARE-driven luciferase activity in HSC-3 cells. Moreover, trans-coniferylaldehyde possesses cytoprotective efficacy against tert-butyl hydroperoxide-induced DNA double-strand breaks in cultured cells, and the chemopreventive potency induced by trans-coniferylaldehyde may be through the activation of kinase signals, including p38, ERK1/2, JNK, MEK1/2, and MSK1/2. In summary, we first identified six antimutagenic constituents from adlay hull. Among them, trans- coniferylaldehyde would be a highly promising agent for cancer chemoprevention and merits further investigation.

Original languageEnglish
Pages (from-to)6444-6452
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number12
DOIs
Publication statusPublished - Jun 22 2011

Fingerprint

Coix
Coix lacryma-jobi
antimutagenic activity
hulls
neoplasms
cultured cells
Neoplasms
Cultured Cells
chemoprevention
Cells
vanillin
tert-Butylhydroperoxide
luciferase
hydroperoxides
mitogen-activated protein kinase
superoxide anion
Double-Stranded DNA Breaks
acetone
Scavenging
Chinese Traditional Medicine

Keywords

  • adlay
  • antimutagenic constituents
  • ARE
  • cancer chemoprevention
  • kinase signal
  • traditional Chinese medicine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Antimutagenic constituents of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) with potential cancer chemopreventive activity. / Chen, Huang Hui; Chiang, Wenchang; Chang, Jang Yang; Chien, Ya Lin; Lee, Ching Kuo; Liu, Ko Jiunn; Cheng, Yen Ting; Chen, Ting Fang; Kuo, Yueh Hsiung; Kuo, Ching Chuan.

In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 12, 22.06.2011, p. 6444-6452.

Research output: Contribution to journalArticle

Chen, Huang Hui ; Chiang, Wenchang ; Chang, Jang Yang ; Chien, Ya Lin ; Lee, Ching Kuo ; Liu, Ko Jiunn ; Cheng, Yen Ting ; Chen, Ting Fang ; Kuo, Yueh Hsiung ; Kuo, Ching Chuan. / Antimutagenic constituents of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) with potential cancer chemopreventive activity. In: Journal of Agricultural and Food Chemistry. 2011 ; Vol. 59, No. 12. pp. 6444-6452.
@article{6b46dd1e47ec40a6902dbf8955181a49,
title = "Antimutagenic constituents of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) with potential cancer chemopreventive activity",
abstract = "Adlay has long been used in traditional Chinese medicine and as a nourishing food. The acetone extract of adlay hull had previously been demonstrated to possess potent antimutagenic activity. The aims of this study were to identify the antimutagenic constituents from adlay hull by using Ames antimutagenic activity-guide isolation procedures and to investigate their chemopreventive efficacies in cultured cells. The results demonstrated that six compounds showing great antimutagenic activity were identified by spectroscopic methods and by comparison with authentic samples to be p-hydroxybenzaldehyde, vanillin, syringaldehyde, trans-coniferylaldehyde, sinapaldehyde, and coixol. Two of them, trans-coniferylaldehyde and sinapaldehyde, exhibit relatively potent scavenging of DPPH radicals, inhibit TPA stimulated superoxide anion generation in neutrophil-like leukocytes, and induce Nrf2/ARE-driven luciferase activity in HSC-3 cells. Moreover, trans-coniferylaldehyde possesses cytoprotective efficacy against tert-butyl hydroperoxide-induced DNA double-strand breaks in cultured cells, and the chemopreventive potency induced by trans-coniferylaldehyde may be through the activation of kinase signals, including p38, ERK1/2, JNK, MEK1/2, and MSK1/2. In summary, we first identified six antimutagenic constituents from adlay hull. Among them, trans- coniferylaldehyde would be a highly promising agent for cancer chemoprevention and merits further investigation.",
keywords = "adlay, antimutagenic constituents, ARE, cancer chemoprevention, kinase signal, traditional Chinese medicine",
author = "Chen, {Huang Hui} and Wenchang Chiang and Chang, {Jang Yang} and Chien, {Ya Lin} and Lee, {Ching Kuo} and Liu, {Ko Jiunn} and Cheng, {Yen Ting} and Chen, {Ting Fang} and Kuo, {Yueh Hsiung} and Kuo, {Ching Chuan}",
year = "2011",
month = "6",
day = "22",
doi = "10.1021/jf200539r",
language = "English",
volume = "59",
pages = "6444--6452",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Antimutagenic constituents of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) with potential cancer chemopreventive activity

AU - Chen, Huang Hui

AU - Chiang, Wenchang

AU - Chang, Jang Yang

AU - Chien, Ya Lin

AU - Lee, Ching Kuo

AU - Liu, Ko Jiunn

AU - Cheng, Yen Ting

AU - Chen, Ting Fang

AU - Kuo, Yueh Hsiung

AU - Kuo, Ching Chuan

PY - 2011/6/22

Y1 - 2011/6/22

N2 - Adlay has long been used in traditional Chinese medicine and as a nourishing food. The acetone extract of adlay hull had previously been demonstrated to possess potent antimutagenic activity. The aims of this study were to identify the antimutagenic constituents from adlay hull by using Ames antimutagenic activity-guide isolation procedures and to investigate their chemopreventive efficacies in cultured cells. The results demonstrated that six compounds showing great antimutagenic activity were identified by spectroscopic methods and by comparison with authentic samples to be p-hydroxybenzaldehyde, vanillin, syringaldehyde, trans-coniferylaldehyde, sinapaldehyde, and coixol. Two of them, trans-coniferylaldehyde and sinapaldehyde, exhibit relatively potent scavenging of DPPH radicals, inhibit TPA stimulated superoxide anion generation in neutrophil-like leukocytes, and induce Nrf2/ARE-driven luciferase activity in HSC-3 cells. Moreover, trans-coniferylaldehyde possesses cytoprotective efficacy against tert-butyl hydroperoxide-induced DNA double-strand breaks in cultured cells, and the chemopreventive potency induced by trans-coniferylaldehyde may be through the activation of kinase signals, including p38, ERK1/2, JNK, MEK1/2, and MSK1/2. In summary, we first identified six antimutagenic constituents from adlay hull. Among them, trans- coniferylaldehyde would be a highly promising agent for cancer chemoprevention and merits further investigation.

AB - Adlay has long been used in traditional Chinese medicine and as a nourishing food. The acetone extract of adlay hull had previously been demonstrated to possess potent antimutagenic activity. The aims of this study were to identify the antimutagenic constituents from adlay hull by using Ames antimutagenic activity-guide isolation procedures and to investigate their chemopreventive efficacies in cultured cells. The results demonstrated that six compounds showing great antimutagenic activity were identified by spectroscopic methods and by comparison with authentic samples to be p-hydroxybenzaldehyde, vanillin, syringaldehyde, trans-coniferylaldehyde, sinapaldehyde, and coixol. Two of them, trans-coniferylaldehyde and sinapaldehyde, exhibit relatively potent scavenging of DPPH radicals, inhibit TPA stimulated superoxide anion generation in neutrophil-like leukocytes, and induce Nrf2/ARE-driven luciferase activity in HSC-3 cells. Moreover, trans-coniferylaldehyde possesses cytoprotective efficacy against tert-butyl hydroperoxide-induced DNA double-strand breaks in cultured cells, and the chemopreventive potency induced by trans-coniferylaldehyde may be through the activation of kinase signals, including p38, ERK1/2, JNK, MEK1/2, and MSK1/2. In summary, we first identified six antimutagenic constituents from adlay hull. Among them, trans- coniferylaldehyde would be a highly promising agent for cancer chemoprevention and merits further investigation.

KW - adlay

KW - antimutagenic constituents

KW - ARE

KW - cancer chemoprevention

KW - kinase signal

KW - traditional Chinese medicine

UR - http://www.scopus.com/inward/record.url?scp=79959224748&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959224748&partnerID=8YFLogxK

U2 - 10.1021/jf200539r

DO - 10.1021/jf200539r

M3 - Article

C2 - 21561091

AN - SCOPUS:79959224748

VL - 59

SP - 6444

EP - 6452

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 12

ER -