Antimicrobial susceptibility testing of Mycobacterium tuberculosis to first-line drugs: Comparisons of the MGIT 960 and BACTEC 460 systems

Tsi S. Huang, Hui Z. Tu, S. S J Lee, Wen Kuei Huang, Yung Ching Liu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The reliability of the Mycobacteria Growth Indicator Tube (MGIT) 960 system for rapid antimicrobial susceptibility testing (AST) of Mycobacterium tuberculosis was evaluated. Forty-seven isolates, including 10 fully susceptible and 37 resistant strains, were tested for susceptibility to the critical concentrations of streptomycin (STR), isoniazid (INH), rifampin (RMP), and ethambutol (EMB), as recommended by the manufacturer. Strains resistant to the critical concentrations were tested with higher concentrations. The results were compared to those obtained by a radiometric method (BACTEC 460TB) and by a conventional agar dilution method, which served as the reference method. Based on these data, we suggest that the following antibiotic concentrations give satisfactory results with the MGIT 960 system: STR, 4.0 μg/ml; INH, 0.1 μg/ml; RMP, 1.0 μg/ml; and EMB, 5.0 μg/ml. The time required to obtain susceptibility results averaged 6.9 days by the MGIT 960 system and 5.4 days by the BACTEC 460TB system; these intervals were not significantly different. This study shows that the MGIT 960 system is a reliable, rapid, automated method for testing the susceptibility of M. tuberculosis isolates to first-line drugs.

Original languageEnglish
Pages (from-to)142-147
Number of pages6
JournalAnnals of Clinical and Laboratory Science
Volume32
Issue number2
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Antimicrobial susceptibility testing
  • Automated analysis
  • Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

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