Antimicrobial susceptibilities and molecular epidemiology of clinical isolates of Clostridium difficile in Taiwan

Yi Chun Lin, Yu Tsung Huang, Pei Jane Tsai, Tai Fen Lee, Nan Yao Lee, Chun Hsing Liao, Shyr Yi Lin, Wen Chien Ko, Po Ren Hsueh

Research output: Contribution to journalArticle

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Abstract

The antimicrobial susceptibility and virulence factors of Clostridium difficile clinical isolates in Taiwan have not previously been reported. One hundred and thirteen isolates were collected from two major teaching hospitals in Taiwan from 2001 to 2009. Molecular typing was performed by an automated repetitive extragenic palindromic sequence-based PCR (rep-PCR) method (DiversiLab; Bacterial Barcodes, Inc., Athens, GA) and PCR ribotyping. Detection of tcdA, tcdB, cdtA, and cdtB genes was performed using a multiplex PCR assay, and gyrA and gyrB genes of moxifloxacin-nonsusceptible isolates were sequenced. All isolates were susceptible to vancomycin and metronidazole. Ninety-five (84%) isolates were susceptible to moxifloxacin, and the MIC90 for nemonoxacin was 4 μg/ml. Tigecycline showed favorable antibacterial activity (MIC90 of 0.06 μg/ml). Thirteen rep-PCR types were identified as a predominant rep-PCR type (type A; non-North American pulsed-field gel electrophoresis type 1 [NAP1], -NAP7, or -NAP8) accounting for 52.2% (59 isolates). Nine of 18 moxifloxacinnonsusceptible isolates belonged to the rep-PCR type A. The rep-PCR type A and C isolates were distinct from NAP1 (ribotype 027) and NAP8 (ribotype 078) as determined by PCR ribotyping. Seventy-four (65%) isolates harbored tcdA and tcdB, and 15 (13%) harbored cdtAB encoding binary toxin. Eleven isolates had a gene deletion in tcdC, including a 39-bp deletion (9 isolates) and an 18-bp deletion (2). In conclusion, dissemination of a predominant C. difficile clone in southern and northern Taiwan was noted. However, no NAP1 (ribotype 027) isolate could be discovered in this study.

Original languageEnglish
Pages (from-to)1701-1705
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume55
Issue number4
DOIs
Publication statusPublished - Apr 2011

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Molecular Epidemiology
Clostridium difficile
Taiwan
Ribotyping
Polymerase Chain Reaction
Molecular Typing
Pulsed Field Gel Electrophoresis
Multiplex Polymerase Chain Reaction
Metronidazole
Gene Deletion
Virulence Factors
Vancomycin
Teaching Hospitals
Genes
Clone Cells

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Antimicrobial susceptibilities and molecular epidemiology of clinical isolates of Clostridium difficile in Taiwan. / Lin, Yi Chun; Huang, Yu Tsung; Tsai, Pei Jane; Lee, Tai Fen; Lee, Nan Yao; Liao, Chun Hsing; Lin, Shyr Yi; Ko, Wen Chien; Hsueh, Po Ren.

In: Antimicrobial Agents and Chemotherapy, Vol. 55, No. 4, 04.2011, p. 1701-1705.

Research output: Contribution to journalArticle

Lin, Yi Chun ; Huang, Yu Tsung ; Tsai, Pei Jane ; Lee, Tai Fen ; Lee, Nan Yao ; Liao, Chun Hsing ; Lin, Shyr Yi ; Ko, Wen Chien ; Hsueh, Po Ren. / Antimicrobial susceptibilities and molecular epidemiology of clinical isolates of Clostridium difficile in Taiwan. In: Antimicrobial Agents and Chemotherapy. 2011 ; Vol. 55, No. 4. pp. 1701-1705.
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abstract = "The antimicrobial susceptibility and virulence factors of Clostridium difficile clinical isolates in Taiwan have not previously been reported. One hundred and thirteen isolates were collected from two major teaching hospitals in Taiwan from 2001 to 2009. Molecular typing was performed by an automated repetitive extragenic palindromic sequence-based PCR (rep-PCR) method (DiversiLab; Bacterial Barcodes, Inc., Athens, GA) and PCR ribotyping. Detection of tcdA, tcdB, cdtA, and cdtB genes was performed using a multiplex PCR assay, and gyrA and gyrB genes of moxifloxacin-nonsusceptible isolates were sequenced. All isolates were susceptible to vancomycin and metronidazole. Ninety-five (84{\%}) isolates were susceptible to moxifloxacin, and the MIC90 for nemonoxacin was 4 μg/ml. Tigecycline showed favorable antibacterial activity (MIC90 of 0.06 μg/ml). Thirteen rep-PCR types were identified as a predominant rep-PCR type (type A; non-North American pulsed-field gel electrophoresis type 1 [NAP1], -NAP7, or -NAP8) accounting for 52.2{\%} (59 isolates). Nine of 18 moxifloxacinnonsusceptible isolates belonged to the rep-PCR type A. The rep-PCR type A and C isolates were distinct from NAP1 (ribotype 027) and NAP8 (ribotype 078) as determined by PCR ribotyping. Seventy-four (65{\%}) isolates harbored tcdA and tcdB, and 15 (13{\%}) harbored cdtAB encoding binary toxin. Eleven isolates had a gene deletion in tcdC, including a 39-bp deletion (9 isolates) and an 18-bp deletion (2). In conclusion, dissemination of a predominant C. difficile clone in southern and northern Taiwan was noted. However, no NAP1 (ribotype 027) isolate could be discovered in this study.",
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