Antimicrobial activities of ceftazidime-avibactam, ceftolozane-tazobactam, and other agents against escherichia coli, klebsiella pneumoniae, and pseudomonas aeruginosa isolated from intensive care units in Taiwan: Results from the surveillance of multicenter antimicrobial resistance in Taiwan in 2016

Chun Hsing Liao, Na Yao Lee, Hung Jen Tang, Susan Shin Jung Lee, Chin Fu Lin, Po Liang Lu, Jiunn Jong Wu, Wen Chien Ko, Wen Sen Lee, Po Ren Hsueh

Research output: Contribution to journalArticle

Abstract

Objective: The aim of this study was to investigate the in vitro antimicrobial susceptibilities of clinically important Gram-negative bacteria from seven intensive care units in Taiwan in 2016. Materials and methods: In total, 300 non-duplicate isolates of Escherichia coli (n=100), Klebsiella pneumoniae (n=100), and Pseudomonas aeruginosa (n=100) collected from 300 patients were studied. The minimum inhibitory concentrations (MICs) of these isolates to antimicrobial agents were determined using the broth microdilution method. Carbapenemase-encoding genes (bla KPC , bla NDM , bla IMP , bla VIM , and bla OXA-48-like ) were studied for the isolates that were not susceptible to any carbapenems. Sequencing analysis of the mcr genes (mcr-1-5) was conducted for all isolates with colistin MICs ≥4 mg/L. Results: Ertapenem non-susceptibility was detected in 3% (n=3) E. coli and 12% (n=12) K. pneumoniae isolates. The susceptibility rates of imipenem, ceftazidime-avibactam (CAZ-AVB), and ceftolozane-tazobactam (CLZ-TAZ) were 99%, 99%, and 88%, respectively, for E. coli, 91%, 100%, and 80%, respectively, for K. pneumoniae, and 66%, 91%, and 93%, respectively, for P. aeruginosa. Carbapenemase-encoding genes were not detected in E. coli, were detected in four (33.3%) K. pneumoniae isolates that were not susceptible to ertapenem (three harboring blaKPC and one harboring blaOXA-48-like), and were not detected in P. aeruginosa isolates that were not susceptible to imipenem. One K. pneumoniae isolate was resistant to colistin (MIC 4 mg/L) and negative for mcrgenes. Conclusion: CAZ-AVB exhibited excellent activity against carbapenem-resistant Enterobacteriaceae, and CLZ-TAZ exhibited good activity against imipenem-resistant P. aeruginosa.

Original languageEnglish
Pages (from-to)545-552
Number of pages8
JournalInfection and Drug Resistance
Volume12
DOIs
Publication statusPublished - Jan 1 2019

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Klebsiella pneumoniae
Taiwan
Pseudomonas aeruginosa
Intensive Care Units
Imipenem
Escherichia coli
Microbial Sensitivity Tests
Colistin
Carbapenems
Genes
Inosine Monophosphate
Enterobacteriaceae
Anti-Infective Agents
Gram-Negative Bacteria
ceftazidime drug combination avibactam
tazobactam drug combination ceftolozane
ertapenem
carbapenemase

Keywords

  • Carbapenem resistance
  • Carbapenemase-encoding genes
  • Mcr
  • Second-generation
  • β-lactam
  • β-lactamase inhibitor combinations

ASJC Scopus subject areas

  • Pharmacology
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Antimicrobial activities of ceftazidime-avibactam, ceftolozane-tazobactam, and other agents against escherichia coli, klebsiella pneumoniae, and pseudomonas aeruginosa isolated from intensive care units in Taiwan : Results from the surveillance of multicenter antimicrobial resistance in Taiwan in 2016. / Liao, Chun Hsing; Lee, Na Yao; Tang, Hung Jen; Lee, Susan Shin Jung; Lin, Chin Fu; Lu, Po Liang; Wu, Jiunn Jong; Ko, Wen Chien; Lee, Wen Sen; Hsueh, Po Ren.

In: Infection and Drug Resistance, Vol. 12, 01.01.2019, p. 545-552.

Research output: Contribution to journalArticle

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title = "Antimicrobial activities of ceftazidime-avibactam, ceftolozane-tazobactam, and other agents against escherichia coli, klebsiella pneumoniae, and pseudomonas aeruginosa isolated from intensive care units in Taiwan: Results from the surveillance of multicenter antimicrobial resistance in Taiwan in 2016",
abstract = "Objective: The aim of this study was to investigate the in vitro antimicrobial susceptibilities of clinically important Gram-negative bacteria from seven intensive care units in Taiwan in 2016. Materials and methods: In total, 300 non-duplicate isolates of Escherichia coli (n=100), Klebsiella pneumoniae (n=100), and Pseudomonas aeruginosa (n=100) collected from 300 patients were studied. The minimum inhibitory concentrations (MICs) of these isolates to antimicrobial agents were determined using the broth microdilution method. Carbapenemase-encoding genes (bla KPC , bla NDM , bla IMP , bla VIM , and bla OXA-48-like ) were studied for the isolates that were not susceptible to any carbapenems. Sequencing analysis of the mcr genes (mcr-1-5) was conducted for all isolates with colistin MICs ≥4 mg/L. Results: Ertapenem non-susceptibility was detected in 3{\%} (n=3) E. coli and 12{\%} (n=12) K. pneumoniae isolates. The susceptibility rates of imipenem, ceftazidime-avibactam (CAZ-AVB), and ceftolozane-tazobactam (CLZ-TAZ) were 99{\%}, 99{\%}, and 88{\%}, respectively, for E. coli, 91{\%}, 100{\%}, and 80{\%}, respectively, for K. pneumoniae, and 66{\%}, 91{\%}, and 93{\%}, respectively, for P. aeruginosa. Carbapenemase-encoding genes were not detected in E. coli, were detected in four (33.3{\%}) K. pneumoniae isolates that were not susceptible to ertapenem (three harboring blaKPC and one harboring blaOXA-48-like), and were not detected in P. aeruginosa isolates that were not susceptible to imipenem. One K. pneumoniae isolate was resistant to colistin (MIC 4 mg/L) and negative for mcrgenes. Conclusion: CAZ-AVB exhibited excellent activity against carbapenem-resistant Enterobacteriaceae, and CLZ-TAZ exhibited good activity against imipenem-resistant P. aeruginosa.",
keywords = "Carbapenem resistance, Carbapenemase-encoding genes, Mcr, Second-generation, β-lactam, β-lactamase inhibitor combinations",
author = "Liao, {Chun Hsing} and Lee, {Na Yao} and Tang, {Hung Jen} and Lee, {Susan Shin Jung} and Lin, {Chin Fu} and Lu, {Po Liang} and Wu, {Jiunn Jong} and Ko, {Wen Chien} and Lee, {Wen Sen} and Hsueh, {Po Ren}",
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T2 - Results from the surveillance of multicenter antimicrobial resistance in Taiwan in 2016

AU - Liao, Chun Hsing

AU - Lee, Na Yao

AU - Tang, Hung Jen

AU - Lee, Susan Shin Jung

AU - Lin, Chin Fu

AU - Lu, Po Liang

AU - Wu, Jiunn Jong

AU - Ko, Wen Chien

AU - Lee, Wen Sen

AU - Hsueh, Po Ren

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Objective: The aim of this study was to investigate the in vitro antimicrobial susceptibilities of clinically important Gram-negative bacteria from seven intensive care units in Taiwan in 2016. Materials and methods: In total, 300 non-duplicate isolates of Escherichia coli (n=100), Klebsiella pneumoniae (n=100), and Pseudomonas aeruginosa (n=100) collected from 300 patients were studied. The minimum inhibitory concentrations (MICs) of these isolates to antimicrobial agents were determined using the broth microdilution method. Carbapenemase-encoding genes (bla KPC , bla NDM , bla IMP , bla VIM , and bla OXA-48-like ) were studied for the isolates that were not susceptible to any carbapenems. Sequencing analysis of the mcr genes (mcr-1-5) was conducted for all isolates with colistin MICs ≥4 mg/L. Results: Ertapenem non-susceptibility was detected in 3% (n=3) E. coli and 12% (n=12) K. pneumoniae isolates. The susceptibility rates of imipenem, ceftazidime-avibactam (CAZ-AVB), and ceftolozane-tazobactam (CLZ-TAZ) were 99%, 99%, and 88%, respectively, for E. coli, 91%, 100%, and 80%, respectively, for K. pneumoniae, and 66%, 91%, and 93%, respectively, for P. aeruginosa. Carbapenemase-encoding genes were not detected in E. coli, were detected in four (33.3%) K. pneumoniae isolates that were not susceptible to ertapenem (three harboring blaKPC and one harboring blaOXA-48-like), and were not detected in P. aeruginosa isolates that were not susceptible to imipenem. One K. pneumoniae isolate was resistant to colistin (MIC 4 mg/L) and negative for mcrgenes. Conclusion: CAZ-AVB exhibited excellent activity against carbapenem-resistant Enterobacteriaceae, and CLZ-TAZ exhibited good activity against imipenem-resistant P. aeruginosa.

AB - Objective: The aim of this study was to investigate the in vitro antimicrobial susceptibilities of clinically important Gram-negative bacteria from seven intensive care units in Taiwan in 2016. Materials and methods: In total, 300 non-duplicate isolates of Escherichia coli (n=100), Klebsiella pneumoniae (n=100), and Pseudomonas aeruginosa (n=100) collected from 300 patients were studied. The minimum inhibitory concentrations (MICs) of these isolates to antimicrobial agents were determined using the broth microdilution method. Carbapenemase-encoding genes (bla KPC , bla NDM , bla IMP , bla VIM , and bla OXA-48-like ) were studied for the isolates that were not susceptible to any carbapenems. Sequencing analysis of the mcr genes (mcr-1-5) was conducted for all isolates with colistin MICs ≥4 mg/L. Results: Ertapenem non-susceptibility was detected in 3% (n=3) E. coli and 12% (n=12) K. pneumoniae isolates. The susceptibility rates of imipenem, ceftazidime-avibactam (CAZ-AVB), and ceftolozane-tazobactam (CLZ-TAZ) were 99%, 99%, and 88%, respectively, for E. coli, 91%, 100%, and 80%, respectively, for K. pneumoniae, and 66%, 91%, and 93%, respectively, for P. aeruginosa. Carbapenemase-encoding genes were not detected in E. coli, were detected in four (33.3%) K. pneumoniae isolates that were not susceptible to ertapenem (three harboring blaKPC and one harboring blaOXA-48-like), and were not detected in P. aeruginosa isolates that were not susceptible to imipenem. One K. pneumoniae isolate was resistant to colistin (MIC 4 mg/L) and negative for mcrgenes. Conclusion: CAZ-AVB exhibited excellent activity against carbapenem-resistant Enterobacteriaceae, and CLZ-TAZ exhibited good activity against imipenem-resistant P. aeruginosa.

KW - Carbapenem resistance

KW - Carbapenemase-encoding genes

KW - Mcr

KW - Second-generation

KW - β-lactam

KW - β-lactamase inhibitor combinations

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