Antihepatoma activity of Artocarpus communis is higher in fractions with high artocarpin content

Cheng Wei Tzeng, Feng Lin Yen, Liang Tzung Lin, Chiang Wen Lee, Ming Hong Yen, Wen Sheng Tzeng, Chun Ching Lin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Extracts from natural plants have been used in traditional medicine for many centuries worldwide. Artocarpus communis is one such plant that has been used to treat liver cirrhosis, hypertension, and diabetes. To our knowledge, this study is the first to investigate the antihepatoma activity of A. communis toward HepG2 and PLC/PRF/5 cells and the first to explore the relationship between antihepatoma activity and the active compound artocarpin content in different fractions of A. communis. A. communis methanol extract and fractions induced dose-dependent reduction of tumor cell viability. DNA laddering analysis revealed that A. communis extract and fractions did not induce apoptosis in HepG2 and PLC/PRF/5 cells. Instead, acridine orange staining revealed that A. communis triggered autophagic cell death in a dose-dependent manner. The antihepatoma activity of A. communis is attributable to artocarpin. The fractions with the highest artocarpin content were also the fractions with the highest antihepatoma activity in the following order: dichloromethane fraction > methanol extract > ethyl acetate fraction > n-butanol fraction > n-hexane fraction. Taken together, A. communis showed antihepatoma activity through autophagic cell death. The effect was related to artocarpin content. Artocarpin could be considered an indicator of the anticancer potential of A. communis extract.

Original languageEnglish
Article number978525
JournalScientific World Journal
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

Artocarpus
methanol
traditional medicine
hypertension
diabetes
Autophagy
apoptosis
Cell death
Programmable logic controllers
tumor
Methanol
acetate
viability
DNA
Acridine Orange
1-Butanol
Methylene Chloride
Plant Extracts
Traditional Medicine
Medical problems

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Antihepatoma activity of Artocarpus communis is higher in fractions with high artocarpin content. / Tzeng, Cheng Wei; Yen, Feng Lin; Lin, Liang Tzung; Lee, Chiang Wen; Yen, Ming Hong; Tzeng, Wen Sheng; Lin, Chun Ching.

In: Scientific World Journal, Vol. 2014, 978525, 2014.

Research output: Contribution to journalArticle

Tzeng, Cheng Wei ; Yen, Feng Lin ; Lin, Liang Tzung ; Lee, Chiang Wen ; Yen, Ming Hong ; Tzeng, Wen Sheng ; Lin, Chun Ching. / Antihepatoma activity of Artocarpus communis is higher in fractions with high artocarpin content. In: Scientific World Journal. 2014 ; Vol. 2014.
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