Antigenotoxic properties of Cassia tea (Cassia tora L.): Mechanism of action and the influence of roasting process

Chi Hao Wu, Gow Chin Yen

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Antigenotoxic properties and the possible mechanisms of water extracts from Cassia tora L. (WECT) treated with different degrees of roasting (unroasted and roasted at 150 and 250°C) were evaluated by the Ames Salmonella/microsome test and the Comet assay. Results indicated that WECT, especially unroasted C. tora (WEUCT), markedly suppressed the mutagenicity of 2-amino-6- methyldipyrido(1,2-a:3′:2′-d)imidazole (Glu-P-1) and 3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole (Trp-P-1). In the Comet assay performed on human lymphocytes, WECT exhibited significant protective effect on Trp-P-1-mediated DNA damage followed the order of unroasted (55%) > roasted at 150°C (42%) > roasted at 250°C (29%). Pre-treatment of the lymphocytes with WEUCT resulted in 30% repression of DNA damage. However, no significant effect on excision-repair system was found during DNA damage expression time in post-treatment scheme (p>0.05). WEUCT showed 84% scavenging effect on oxygen free radicals generated in the activation process of mutagen detected by electron paramagentic resonance system. Two possible mechanisms were considered: (1) neutralization the reactive intermediate of Trp-P-1; and (2) protecting cells directly as an antioxidant that scavenge the oxygen radicals from the activation process of mutagen. The individual anthraquinone content in extracts of C. tora was measured by HPLC. Three anthraquinones, chrysophanol, emodin and rhein, have been detected under experimental conditions. The anthraquinone content decreased with increased roasting temperature. Each of these anthraquinones demonstrated significant antigenotoxicity against Trp-P-1 in the Comet assay. In conclusion, our data suggest that the decrease in antigenotoxic potency of roasted C. tora was related to the reduction in their anthraquinones.

Original languageEnglish
Pages (from-to)85-101
Number of pages17
JournalLife Sciences
Volume76
Issue number1
DOIs
Publication statusPublished - Nov 19 2004
Externally publishedYes

Fingerprint

Cassia
Anthraquinones
Tea
2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole
Comet Assay
DNA Damage
Assays
Lymphocytes
Mutagens
Water
Reactive Oxygen Species
DNA
Chemical activation
Electron resonance
Emodin
Carbolines
Salmonella
Scavenging
Microsomes
DNA Repair

Keywords

  • Anthraquinones
  • Antigenotoxicity
  • Cassia tora L.
  • Food mutagen
  • Mechanism
  • Roasting

ASJC Scopus subject areas

  • Pharmacology

Cite this

Antigenotoxic properties of Cassia tea (Cassia tora L.) : Mechanism of action and the influence of roasting process. / Wu, Chi Hao; Yen, Gow Chin.

In: Life Sciences, Vol. 76, No. 1, 19.11.2004, p. 85-101.

Research output: Contribution to journalArticle

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abstract = "Antigenotoxic properties and the possible mechanisms of water extracts from Cassia tora L. (WECT) treated with different degrees of roasting (unroasted and roasted at 150 and 250°C) were evaluated by the Ames Salmonella/microsome test and the Comet assay. Results indicated that WECT, especially unroasted C. tora (WEUCT), markedly suppressed the mutagenicity of 2-amino-6- methyldipyrido(1,2-a:3′:2′-d)imidazole (Glu-P-1) and 3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole (Trp-P-1). In the Comet assay performed on human lymphocytes, WECT exhibited significant protective effect on Trp-P-1-mediated DNA damage followed the order of unroasted (55{\%}) > roasted at 150°C (42{\%}) > roasted at 250°C (29{\%}). Pre-treatment of the lymphocytes with WEUCT resulted in 30{\%} repression of DNA damage. However, no significant effect on excision-repair system was found during DNA damage expression time in post-treatment scheme (p>0.05). WEUCT showed 84{\%} scavenging effect on oxygen free radicals generated in the activation process of mutagen detected by electron paramagentic resonance system. Two possible mechanisms were considered: (1) neutralization the reactive intermediate of Trp-P-1; and (2) protecting cells directly as an antioxidant that scavenge the oxygen radicals from the activation process of mutagen. The individual anthraquinone content in extracts of C. tora was measured by HPLC. Three anthraquinones, chrysophanol, emodin and rhein, have been detected under experimental conditions. The anthraquinone content decreased with increased roasting temperature. Each of these anthraquinones demonstrated significant antigenotoxicity against Trp-P-1 in the Comet assay. In conclusion, our data suggest that the decrease in antigenotoxic potency of roasted C. tora was related to the reduction in their anthraquinones.",
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