Antifibrotic effect of bletilla striata polysaccharide-resveratrol-impregnated dual-layer carboxymethyl cellulose-based sponge for the prevention of epidural fibrosis after laminectomy

Hsuan Yu Chen, Tzu Chieh Lin, Chih Yung Chiang, Shiuan Li Wey, Feng Huei Lin, Kai Chiang Yang, Chih Hao Chang, Ming Hsiao Hu

Research output: Contribution to journalArticlepeer-review

Abstract

The application of antifibrotic materials can alleviate epidural fibrosis by restricting excessive fibroblast proliferation and mitigating scar tissue formation. Here, a biodegradable car-boxymethyl cellulose (CMC)-Bletilla striata polysaccharide (BSP)-resveratrol (RES) sponge was fabricated to inhibit scar tissue formation post laminectomy surgery. Fibroblasts NIH/3T3, myoblasts C2C12, neural cells PC-12, and Schwann cells RSC96 were used to evaluate the in vitro cytocompati-bility. Laminectomies on 10 Sprague–Dawley rats with/without the application of the CMC-BSP-RES sponge were performed. The severity of adhesion between the dura mater and formed scar tissue was qualitatively scored. All cell lines exhibited good viability with no significant difference in cytotoxicity when cultured with variable extractions of the CMC-BSP-RES sponge. S100a4 and P4hb expressions were downregulated in NIH/3T3 cultured in the CMC-BSP-RES sponge, implying that this sponge potentially inhibits fibroblast activity. No post-operative shrinkage or dura mater expan-sion along the surgical site was detected. The peel-off tests revealed that the tenacity of adhesion de-creased. Histopathological examinations verified that the average number of fibroblasts in the CMC-BSP-RES group considerably decreased. The CMC-BSP-RES sponge is a biocompatible and effective material for alleviating post-operative epidural fibrosis and mitigating fibroblast expression following laminectomy.

Original languageEnglish
Article number2129
JournalPolymers
Volume13
Issue number13
DOIs
Publication statusPublished - Jul 2021

Keywords

  • Bletilla striata
  • Carboxymethyl cellulose
  • Epidural fibrosis
  • Laminectomy
  • Resveratrol

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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