Anticancer activity of rhamnoallosan against DU-145 cells is kinetically complementary to coexisting polyphenolics in psidium guajava budding leaves

Kuan Chou Chen, Chiu Lan Hsieh, Kuan Dar Huang, Yaw Bee Ker, Charng Cherng Chyau, Robert Y. Peng

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Psldlum guajava L. is a valuable farm fruit plant having many medicinal uses. Previously its budding leaves (PE) were shown to contain huge amounts of soluble polyphenols (SP) including (in mg/g) gallic acid (348), catechin (102), epicatechin (60), rutin (100), quercetin (102), and rutin (100) and to exhibit potent anticancer activity. However, reconstitution of these polyphenols recovered only 40% of the original bioactivity, and the soluble carbohydrate (SC) portion in PE was suspected to contribute the remaining. PE contained a novel rhamnoallosan, which had a carbohydrate/protein (w/w) ratio = 29.06%/10.27% (=2.83, average molecular mass of 5029 kDa), characteristically evidencing a peptidoglycan, consisting of a composition (mole % ratio) of rhamnose/allose/arabinose/tallose/ xylose/fucose/glucose/mannose/galactose = 36.05:24.24:8.76:7.95:7.37:5.90:3.69:3.19:2.85 and of amino acid (in wt %) glycine/leucine/proline/alanine/methionine/isoleucine/valine/histidine/tyrosine/ phenylalanine/cysteine/aspartic acid/lysine/glutamic acid=37.12:12.68:10.05:8. 97:5.99:4.89:4.83:4.25: 4.05:2.78:1.86:1.10:0.73:0.70. Kinetic analysis showed comparable apparent cell-killing rate coefficients (K app) to be 4.03 x 10 3 and 2.92 x 10 3 cells mg -1 h -1 respectively, by SP and SC, evidencing the complementary anti-DU-145 bioactivity in nature.

Original languageEnglish
Pages (from-to)6114-6122
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number14
DOIs
Publication statusPublished - Jul 22 2009

Fingerprint

Psidium
Psidium guajava
Polyphenols
Rutin
polyphenols
Ala-Leu-Pro-Met
Catechin
Carbohydrates
rutin
Bioactivity
carbohydrates
allose
leaves
Rhamnose
Gallic Acid
Arabinose
Fucose
fucose
Peptidoglycan
Isoleucine

Keywords

  • Alióse
  • Prostate cancer
  • Psldlum guajava budding leaves
  • Rhamnoallosan peptldoglycans
  • Rhamnose

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Anticancer activity of rhamnoallosan against DU-145 cells is kinetically complementary to coexisting polyphenolics in psidium guajava budding leaves. / Chen, Kuan Chou; Hsieh, Chiu Lan; Huang, Kuan Dar; Ker, Yaw Bee; Chyau, Charng Cherng; Peng, Robert Y.

In: Journal of Agricultural and Food Chemistry, Vol. 57, No. 14, 22.07.2009, p. 6114-6122.

Research output: Contribution to journalArticle

Chen, Kuan Chou ; Hsieh, Chiu Lan ; Huang, Kuan Dar ; Ker, Yaw Bee ; Chyau, Charng Cherng ; Peng, Robert Y. / Anticancer activity of rhamnoallosan against DU-145 cells is kinetically complementary to coexisting polyphenolics in psidium guajava budding leaves. In: Journal of Agricultural and Food Chemistry. 2009 ; Vol. 57, No. 14. pp. 6114-6122.
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abstract = "Psldlum guajava L. is a valuable farm fruit plant having many medicinal uses. Previously its budding leaves (PE) were shown to contain huge amounts of soluble polyphenols (SP) including (in mg/g) gallic acid (348), catechin (102), epicatechin (60), rutin (100), quercetin (102), and rutin (100) and to exhibit potent anticancer activity. However, reconstitution of these polyphenols recovered only 40{\%} of the original bioactivity, and the soluble carbohydrate (SC) portion in PE was suspected to contribute the remaining. PE contained a novel rhamnoallosan, which had a carbohydrate/protein (w/w) ratio = 29.06{\%}/10.27{\%} (=2.83, average molecular mass of 5029 kDa), characteristically evidencing a peptidoglycan, consisting of a composition (mole {\%} ratio) of rhamnose/allose/arabinose/tallose/ xylose/fucose/glucose/mannose/galactose = 36.05:24.24:8.76:7.95:7.37:5.90:3.69:3.19:2.85 and of amino acid (in wt {\%}) glycine/leucine/proline/alanine/methionine/isoleucine/valine/histidine/tyrosine/ phenylalanine/cysteine/aspartic acid/lysine/glutamic acid=37.12:12.68:10.05:8. 97:5.99:4.89:4.83:4.25: 4.05:2.78:1.86:1.10:0.73:0.70. Kinetic analysis showed comparable apparent cell-killing rate coefficients (K app) to be 4.03 x 10 3 and 2.92 x 10 3 cells mg -1 h -1 respectively, by SP and SC, evidencing the complementary anti-DU-145 bioactivity in nature.",
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AB - Psldlum guajava L. is a valuable farm fruit plant having many medicinal uses. Previously its budding leaves (PE) were shown to contain huge amounts of soluble polyphenols (SP) including (in mg/g) gallic acid (348), catechin (102), epicatechin (60), rutin (100), quercetin (102), and rutin (100) and to exhibit potent anticancer activity. However, reconstitution of these polyphenols recovered only 40% of the original bioactivity, and the soluble carbohydrate (SC) portion in PE was suspected to contribute the remaining. PE contained a novel rhamnoallosan, which had a carbohydrate/protein (w/w) ratio = 29.06%/10.27% (=2.83, average molecular mass of 5029 kDa), characteristically evidencing a peptidoglycan, consisting of a composition (mole % ratio) of rhamnose/allose/arabinose/tallose/ xylose/fucose/glucose/mannose/galactose = 36.05:24.24:8.76:7.95:7.37:5.90:3.69:3.19:2.85 and of amino acid (in wt %) glycine/leucine/proline/alanine/methionine/isoleucine/valine/histidine/tyrosine/ phenylalanine/cysteine/aspartic acid/lysine/glutamic acid=37.12:12.68:10.05:8. 97:5.99:4.89:4.83:4.25: 4.05:2.78:1.86:1.10:0.73:0.70. Kinetic analysis showed comparable apparent cell-killing rate coefficients (K app) to be 4.03 x 10 3 and 2.92 x 10 3 cells mg -1 h -1 respectively, by SP and SC, evidencing the complementary anti-DU-145 bioactivity in nature.

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