Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity

Y. S. Lin, C. F. Lin, Y. T. Fang, Y. M. Kuo, P. C. Liao, T. M. Yeh, K. Y. Hwa, C. C K Shieh, J. H. Yen, H. J. Wang, I. J. Su, H. Y. Lei

Research output: Contribution to journalArticle

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Abstract

Both viral effect and immune-mediated mechanism are involved in the pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. In this study, we showed that in SARS patient sera there were autoantibodies (autoAbs) that reacted with A549 cells, the type-2 pneumocytes, and that these autoAbs were mainly IgG. The autoAbs were detectable 20 days after fever onset. Tests of non-SARS-pneumonia patients did not show the same autoAb production as in SARS patients. After sera IgG bound to A549 cells, cytotoxicity was induced. Cell cytotoxicity and the anti-epithelial cell IgG level were positively correlated. Preabsorption and binding assays indicated the existence of cross-reactive epitopes on SARS-CoV spike protein domain 2 (S2). Furthermore, treatment of A549 cells with anti-S2 Abs and IFN-γ resulted in an increase in the adherence of human peripheral blood mononuclear cells to these epithelial cells. Taken together, we have demonstrated that the anti-S2 Abs in SARS patient sera cause cytotoxic injury as well as enhance immune cell adhesion to epithelial cells. The onset of autoimmune responses in SARS-CoV infection may be implicated in SARS pathogenesis.

Original languageEnglish
Pages (from-to)500-508
Number of pages9
JournalClinical and Experimental Immunology
Volume141
Issue number3
DOIs
Publication statusPublished - Sep 2005
Externally publishedYes

Fingerprint

Coronavirus Spike Glycoproteins
Severe Acute Respiratory Syndrome
Epithelial Cells
Lung
Antibodies
Autoantibodies
Immunoglobulin G
Serum
Coronavirus Infections
Alveolar Epithelial Cells
Protein Domains
Autoimmunity
Cell Adhesion
Epitopes
Blood Cells
Pneumonia
Fever

Keywords

  • Autoantibody
  • Cytotoxicity
  • Lung epithelial cell
  • SARS
  • Spike protein

ASJC Scopus subject areas

  • Immunology

Cite this

Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity. / Lin, Y. S.; Lin, C. F.; Fang, Y. T.; Kuo, Y. M.; Liao, P. C.; Yeh, T. M.; Hwa, K. Y.; Shieh, C. C K; Yen, J. H.; Wang, H. J.; Su, I. J.; Lei, H. Y.

In: Clinical and Experimental Immunology, Vol. 141, No. 3, 09.2005, p. 500-508.

Research output: Contribution to journalArticle

Lin, Y. S. ; Lin, C. F. ; Fang, Y. T. ; Kuo, Y. M. ; Liao, P. C. ; Yeh, T. M. ; Hwa, K. Y. ; Shieh, C. C K ; Yen, J. H. ; Wang, H. J. ; Su, I. J. ; Lei, H. Y. / Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity. In: Clinical and Experimental Immunology. 2005 ; Vol. 141, No. 3. pp. 500-508.
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