Antibacterial nanostructured composite films for biomedical applications: microstructural characteristics, biocompatibility, and antibacterial mechanisms

Fei Peng Lee, Peter Da-yun Wang, Li Kai Chen, Chun Ming Kung, Yi Chien Wu, Keng Liang Ou, Chih Hua Yu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Hydrogenated Cu-incorporated diamond-like carbon (a-C:H/Cu) films were prepared in the present study using a radio-frequency plasma magnetron sputtering system at various CH4/Ar gas ratios. The a-C:H/Cu films were characterized by scanning electron microscopy, atomic force microscopy, Raman spectroscopy, transmission electron microscopy, nano-indentation and a contact angle goniometer. The antibacterial properties and cell cytotoxicity of a-C:H/Cu films were evaluated as per JIS Z2801:2010 and ISO 10993-5 specifications, respectively. The analytical results revealed that the production of a-C:H/Cu films varied with the CH4/Ar ratio, and the phase transformation (amorphous-like → nano-polycrystalline structure) was induced by Cu doping/ion bombardment and radical reactions. Moreover, it was found that the microhardness of the a-C:H/Cu films decreased with increasing Ar fraction in the gas ratio. The a-C:H/Cu films exhibited a high hydrophobic surface feature. The film which contained 77.3 ± 4.4 at.% Cu did not influence cell adhesion and proliferation behaviors. Antibacterial tests also demonstrated that a-C:H/Cu films possessed excellent antibacterial properties. Therefore, a-C:H/Cu films could be developed as promising antibacterial coatings for biomedical applications.

Original languageEnglish
Pages (from-to)295-305
Number of pages11
JournalBiofouling
Volume29
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

biocompatibility
films (materials)
Gases
Diamond
Raman Spectrum Analysis
Atomic Force Microscopy
Transmission Electron Microscopy
Radio
Cell Adhesion
Electron Scanning Microscopy
Carbon
Cell Proliferation
Ions
antibacterial properties
gases
Raman spectroscopy
contact angle
atomic force microscopy
indentation
phase transition

Keywords

  • antibacterial properties
  • biocompatibility
  • contact angle
  • hydrogenated diamond-like carbon
  • microstructure

ASJC Scopus subject areas

  • Aquatic Science
  • Water Science and Technology
  • Applied Microbiology and Biotechnology

Cite this

Antibacterial nanostructured composite films for biomedical applications : microstructural characteristics, biocompatibility, and antibacterial mechanisms. / Lee, Fei Peng; Wang, Peter Da-yun; Chen, Li Kai; Kung, Chun Ming; Wu, Yi Chien; Ou, Keng Liang; Yu, Chih Hua.

In: Biofouling, Vol. 29, No. 3, 03.2013, p. 295-305.

Research output: Contribution to journalArticle

Lee, Fei Peng ; Wang, Peter Da-yun ; Chen, Li Kai ; Kung, Chun Ming ; Wu, Yi Chien ; Ou, Keng Liang ; Yu, Chih Hua. / Antibacterial nanostructured composite films for biomedical applications : microstructural characteristics, biocompatibility, and antibacterial mechanisms. In: Biofouling. 2013 ; Vol. 29, No. 3. pp. 295-305.
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