Antibacterial activities of Anisomeles indica constituents and their inhibition effect on Helicobacter pylori-induced inflammation in human gastric epithelial cells

Yerra Koteswara Rao, Hsiu Man Lien, Yu Hsin Lin, Yuan Man Hsu, Chi Tai Yeh, Chia Chang Chen, Chih Ho Lai, Yew Min Tzeng

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The antibacterial activity of Anisomeles indica extract, and its isolated constituents against Helicobacter pylori growth were examined. Among tested, ethanol extract, pure constituents ovatodiolide (OVT) followed by acteoside, isoacteoside, and terniflorin showed potent antimicrobial activity. OVT demonstrated bactericide activity against H. pylori reference, as well as multidrug-resistant strains. On the other side, in vitro H. pylori-infection model revealed that OVT inhibited the H. pylori bacteria adhesion and invasion to human gastric epithelial (AGS) cells. In addition, OVT inhibited the H. pylori-induced inflammatory response by the reduced nuclear factor (NF)-κB activation and interleukin (IL)-8 expressions in H. pylori-infected AGS cells. Furthermore, OVT attenuated the cytotoxin-associated gene A (CagA) functions by reduced CagA translocation, phosphorylation, and caused hummingbird phenotype of AGS cells. These results indicate that OVT might be useful as food supplement or drug development for H. pylori complications.

Original languageEnglish
Pages (from-to)780-787
Number of pages8
JournalFood Chemistry
Volume132
Issue number2
DOIs
Publication statusPublished - May 15 2012

Fingerprint

Anisomeles indica
Helicobacter pylori
Stomach
stomach
epithelial cells
inflammation
Epithelial Cells
Inflammation
cytotoxins
Cytotoxins
Genes
Bactericides
Phosphorylation
hummingbirds
Interleukin-8
interleukin-8
extracts
Helicobacter Infections
Dietary Supplements
ovatodiolide

Keywords

  • AGS cells
  • Anisomeles indica
  • Cytotoxin-associated gene A
  • Helicobacter pylori
  • Interleukin-8
  • Nuclear factor kappa B
  • Ovatodiolide

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Antibacterial activities of Anisomeles indica constituents and their inhibition effect on Helicobacter pylori-induced inflammation in human gastric epithelial cells. / Rao, Yerra Koteswara; Lien, Hsiu Man; Lin, Yu Hsin; Hsu, Yuan Man; Yeh, Chi Tai; Chen, Chia Chang; Lai, Chih Ho; Tzeng, Yew Min.

In: Food Chemistry, Vol. 132, No. 2, 15.05.2012, p. 780-787.

Research output: Contribution to journalArticle

Rao, Yerra Koteswara ; Lien, Hsiu Man ; Lin, Yu Hsin ; Hsu, Yuan Man ; Yeh, Chi Tai ; Chen, Chia Chang ; Lai, Chih Ho ; Tzeng, Yew Min. / Antibacterial activities of Anisomeles indica constituents and their inhibition effect on Helicobacter pylori-induced inflammation in human gastric epithelial cells. In: Food Chemistry. 2012 ; Vol. 132, No. 2. pp. 780-787.
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