Antibacterial activities of Anisomeles indica constituents and their inhibition effect on Helicobacter pylori-induced inflammation in human gastric epithelial cells

Yerra Koteswara Rao; Hsiu Man Lien; Yu Hsin Lin; Yuan Man Hsu; Chi Tai Yeh; Chia Chang Chen; Chih Ho Lai; Yew Min Tzeng

doi:10.1016/j.foodchem.2011.11.037

Antibacterial activities of Anisomeles indica constituents and their inhibition effect on Helicobacter pylori-induced inflammation in human gastric epithelial cells

Yerra Koteswara Rao, Hsiu Man Lien, Yu Hsin Lin, Yuan Man Hsu, Chi Tai Yeh, Chia Chang Chen, Chih Ho Lai, Yew Min Tzeng

Graduate Institute of Clinical Medicine

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

The antibacterial activity of Anisomeles indica extract, and its isolated constituents against Helicobacter pylori growth were examined. Among tested, ethanol extract, pure constituents ovatodiolide (OVT) followed by acteoside, isoacteoside, and terniflorin showed potent antimicrobial activity. OVT demonstrated bactericide activity against H. pylori reference, as well as multidrug-resistant strains. On the other side, in vitro H. pylori-infection model revealed that OVT inhibited the H. pylori bacteria adhesion and invasion to human gastric epithelial (AGS) cells. In addition, OVT inhibited the H. pylori-induced inflammatory response by the reduced nuclear factor (NF)-κB activation and interleukin (IL)-8 expressions in H. pylori-infected AGS cells. Furthermore, OVT attenuated the cytotoxin-associated gene A (CagA) functions by reduced CagA translocation, phosphorylation, and caused hummingbird phenotype of AGS cells. These results indicate that OVT might be useful as food supplement or drug development for H. pylori complications.

Original language	English
Pages (from-to)	780-787
Number of pages	8
Journal	Food Chemistry
Volume	132
Issue number	2
DOIs	https://doi.org/10.1016/j.foodchem.2011.11.037
Publication status	Published - May 15 2012

Keywords

AGS cells
Anisomeles indica
Cytotoxin-associated gene A
Helicobacter pylori
Interleukin-8
Nuclear factor kappa B
Ovatodiolide

ASJC Scopus subject areas

Food Science
Analytical Chemistry

Access to Document

10.1016/j.foodchem.2011.11.037

Cite this

@article{8ea05806a3c6473094d6d9f34b1f14bf,

title = "Antibacterial activities of Anisomeles indica constituents and their inhibition effect on Helicobacter pylori-induced inflammation in human gastric epithelial cells",

abstract = "The antibacterial activity of Anisomeles indica extract, and its isolated constituents against Helicobacter pylori growth were examined. Among tested, ethanol extract, pure constituents ovatodiolide (OVT) followed by acteoside, isoacteoside, and terniflorin showed potent antimicrobial activity. OVT demonstrated bactericide activity against H. pylori reference, as well as multidrug-resistant strains. On the other side, in vitro H. pylori-infection model revealed that OVT inhibited the H. pylori bacteria adhesion and invasion to human gastric epithelial (AGS) cells. In addition, OVT inhibited the H. pylori-induced inflammatory response by the reduced nuclear factor (NF)-κB activation and interleukin (IL)-8 expressions in H. pylori-infected AGS cells. Furthermore, OVT attenuated the cytotoxin-associated gene A (CagA) functions by reduced CagA translocation, phosphorylation, and caused hummingbird phenotype of AGS cells. These results indicate that OVT might be useful as food supplement or drug development for H. pylori complications.",

keywords = "AGS cells, Anisomeles indica, Cytotoxin-associated gene A, Helicobacter pylori, Interleukin-8, Nuclear factor kappa B, Ovatodiolide",

author = "Rao, {Yerra Koteswara} and Lien, {Hsiu Man} and Lin, {Yu Hsin} and Hsu, {Yuan Man} and Yeh, {Chi Tai} and Chen, {Chia Chang} and Lai, {Chih Ho} and Tzeng, {Yew Min}",

note = "Funding Information: This study was supported by the Grants from National Science Council of Taiwan ( NSC 98-1B05 ; NSC 98-2324-B-324-001 , NSC 99-2811-M-324-003 , and NSC 100-2313-B-039-003 ), China Medical University ( CMU99-ASIA-21 , CMU99-S-09 ), and by Tomorrow Medical Foundation . We thank Chin-Jui Tseng, Chao-Lu Huang, Te-Wei Tzeng, Dr. Yu-Ting Sing and Dr. Li-Yu Chen for their expert technical assistance. ",

year = "2012",

month = may,

day = "15",

doi = "10.1016/j.foodchem.2011.11.037",

language = "English",

volume = "132",

pages = "780--787",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier Ltd",

number = "2",

}

TY - JOUR

T1 - Antibacterial activities of Anisomeles indica constituents and their inhibition effect on Helicobacter pylori-induced inflammation in human gastric epithelial cells

AU - Rao, Yerra Koteswara

AU - Lien, Hsiu Man

AU - Lin, Yu Hsin

AU - Hsu, Yuan Man

AU - Yeh, Chi Tai

AU - Chen, Chia Chang

AU - Lai, Chih Ho

AU - Tzeng, Yew Min

N1 - Funding Information: This study was supported by the Grants from National Science Council of Taiwan ( NSC 98-1B05 ; NSC 98-2324-B-324-001 , NSC 99-2811-M-324-003 , and NSC 100-2313-B-039-003 ), China Medical University ( CMU99-ASIA-21 , CMU99-S-09 ), and by Tomorrow Medical Foundation . We thank Chin-Jui Tseng, Chao-Lu Huang, Te-Wei Tzeng, Dr. Yu-Ting Sing and Dr. Li-Yu Chen for their expert technical assistance.

PY - 2012/5/15

Y1 - 2012/5/15

N2 - The antibacterial activity of Anisomeles indica extract, and its isolated constituents against Helicobacter pylori growth were examined. Among tested, ethanol extract, pure constituents ovatodiolide (OVT) followed by acteoside, isoacteoside, and terniflorin showed potent antimicrobial activity. OVT demonstrated bactericide activity against H. pylori reference, as well as multidrug-resistant strains. On the other side, in vitro H. pylori-infection model revealed that OVT inhibited the H. pylori bacteria adhesion and invasion to human gastric epithelial (AGS) cells. In addition, OVT inhibited the H. pylori-induced inflammatory response by the reduced nuclear factor (NF)-κB activation and interleukin (IL)-8 expressions in H. pylori-infected AGS cells. Furthermore, OVT attenuated the cytotoxin-associated gene A (CagA) functions by reduced CagA translocation, phosphorylation, and caused hummingbird phenotype of AGS cells. These results indicate that OVT might be useful as food supplement or drug development for H. pylori complications.

AB - The antibacterial activity of Anisomeles indica extract, and its isolated constituents against Helicobacter pylori growth were examined. Among tested, ethanol extract, pure constituents ovatodiolide (OVT) followed by acteoside, isoacteoside, and terniflorin showed potent antimicrobial activity. OVT demonstrated bactericide activity against H. pylori reference, as well as multidrug-resistant strains. On the other side, in vitro H. pylori-infection model revealed that OVT inhibited the H. pylori bacteria adhesion and invasion to human gastric epithelial (AGS) cells. In addition, OVT inhibited the H. pylori-induced inflammatory response by the reduced nuclear factor (NF)-κB activation and interleukin (IL)-8 expressions in H. pylori-infected AGS cells. Furthermore, OVT attenuated the cytotoxin-associated gene A (CagA) functions by reduced CagA translocation, phosphorylation, and caused hummingbird phenotype of AGS cells. These results indicate that OVT might be useful as food supplement or drug development for H. pylori complications.

KW - AGS cells

KW - Anisomeles indica

KW - Cytotoxin-associated gene A

KW - Helicobacter pylori

KW - Interleukin-8

KW - Nuclear factor kappa B

KW - Ovatodiolide

UR - http://www.scopus.com/inward/record.url?scp=84855704209&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855704209&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2011.11.037

DO - 10.1016/j.foodchem.2011.11.037

M3 - Article

AN - SCOPUS:84855704209

SN - 0308-8146

VL - 132

SP - 780

EP - 787

JO - Food Chemistry

JF - Food Chemistry

IS - 2

ER -

Antibacterial activities of Anisomeles indica constituents and their inhibition effect on Helicobacter pylori-induced inflammation in human gastric epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this