Antiangiogenic activity of indole-3-carbinol in endothelial cells stimulated with activated macrophages

Mei Ling Wang, Chun-Kuang Shih, H. P. Chang, Yue-Hwa Chen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effect of indole-3-carbinol (I3C), a major indolic metabolite in cruciferous vegetables, on lipopolysaccharide (LPS)-activated macrophage-induced tube formation and its associated factors in endothelial EA hy926 cells was investigated. LPS significantly enhanced the capillary-like structure of endothelial cells (ECs) co-cultured with macrophages, but no such effect was observed in single-cultured ECs. I3C, on the other hand, suppressed such enhancement in concert with decreased secretions of vascular endothelial growth factor (VEGF), nitric oxide (NO), interleukin-6 (IL-6), and matrix metalloproteinases (MMPs). The results obtained from cultivating ECs with conditioned medium (CM) collected from macrophages suggested that both ECs and macrophages were inactivated by I3C. These results indicate that I3C from cruciferous vegetables may possess potential roles in preventing inflammation-associated angiogenic diseases.

Original languageEnglish
Pages (from-to)811-820
Number of pages10
JournalFood Chemistry
Volume134
Issue number2
DOIs
Publication statusPublished - Sep 15 2012

Fingerprint

Macrophages
Endothelial cells
indoles
endothelial cells
macrophages
Endothelial Cells
Vegetables
lipopolysaccharides
Lipopolysaccharides
vegetables
vascular endothelial growth factors
metalloproteinases
Conditioned Culture Medium
Metabolites
Matrix Metalloproteinases
interleukin-6
cultured cells
Vascular Endothelial Growth Factor A
nitric oxide
Cultured Cells

Keywords

  • Activated macrophages
  • Angiogenesis
  • Co-culture
  • Cruciferous vegetables
  • Indole-3-carbinol
  • Tube formation

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Antiangiogenic activity of indole-3-carbinol in endothelial cells stimulated with activated macrophages. / Wang, Mei Ling; Shih, Chun-Kuang; Chang, H. P.; Chen, Yue-Hwa.

In: Food Chemistry, Vol. 134, No. 2, 15.09.2012, p. 811-820.

Research output: Contribution to journalArticle

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