Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo

Yeung Leung Cheng, Shih Chun Lee, Shinn Zong Lin, Wen Liang Chang, Yi Lin Chen, Nu Man Tsai, Yao Chi Liu, Ching Tzao, Dah Shyong Yu, Horng Jyh Harn

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48 Citations (Scopus)

Abstract

Nan-Chai-Hu, the root of Bupleurum scorzonerifolium, is a traditional Chinese herb used in treatment of liver diseases such as hepatitis and cirrhosis. We recently reported that the acetone extract of B. scorzonerifolium (BS-AE) could inhibit proliferation and induce apoptosis in A549 human lung cancer cells. We further examined its anti-proliferative mechanisms and in vivo anticancer effect. Our results showed that BS-AE had the ability to cause cell cycle arrest in G2/M phase, inducing tubulin polymerization, and activating caspase-3 and -9 in A549 cells. BS-AE-induced apoptosis could be blocked by the broad caspase inhibitor z-VAD-fmk in majority. The result of in vivo study showed that BS-AE could suppress growth in A549 subcutaneous xenograft tumors. These results indicate that BS-AE exerts antiproliferative effects on A549 cells in vitro and in vivo, and prompted us to further evaluate and elucidate the chemical composition profile of BS-AE.

Original languageEnglish
Pages (from-to)183-193
Number of pages11
JournalCancer Letters
Volume222
Issue number2
DOIs
Publication statusPublished - May 26 2005
Externally publishedYes

Keywords

  • Apoptosis
  • Bupleurum scorzonerifolium
  • Caspases
  • Cell cycle
  • Lung cancer
  • Tubulin

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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  • Cite this

    Cheng, Y. L., Lee, S. C., Lin, S. Z., Chang, W. L., Chen, Y. L., Tsai, N. M., Liu, Y. C., Tzao, C., Yu, D. S., & Harn, H. J. (2005). Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo. Cancer Letters, 222(2), 183-193. https://doi.org/10.1016/j.canlet.2004.10.015