Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo

Yeung Leung Cheng, Shih Chun Lee, Shinn Zong Lin, Wen Liang Chang, Yi Lin Chen, Nu Man Tsai, Yao Chi Liu, Ching Tzao, Dah Shyong Yu, Horng Jyh Harn

Research output: Contribution to journalArticle

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Abstract

Nan-Chai-Hu, the root of Bupleurum scorzonerifolium, is a traditional Chinese herb used in treatment of liver diseases such as hepatitis and cirrhosis. We recently reported that the acetone extract of B. scorzonerifolium (BS-AE) could inhibit proliferation and induce apoptosis in A549 human lung cancer cells. We further examined its anti-proliferative mechanisms and in vivo anticancer effect. Our results showed that BS-AE had the ability to cause cell cycle arrest in G2/M phase, inducing tubulin polymerization, and activating caspase-3 and -9 in A549 cells. BS-AE-induced apoptosis could be blocked by the broad caspase inhibitor z-VAD-fmk in majority. The result of in vivo study showed that BS-AE could suppress growth in A549 subcutaneous xenograft tumors. These results indicate that BS-AE exerts antiproliferative effects on A549 cells in vitro and in vivo, and prompted us to further evaluate and elucidate the chemical composition profile of BS-AE.

Original languageEnglish
Pages (from-to)183-193
Number of pages11
JournalCancer Letters
Volume222
Issue number2
DOIs
Publication statusPublished - May 26 2005
Externally publishedYes

Fingerprint

Bupleurum
Lung Neoplasms
Apoptosis
Caspase Inhibitors
Caspase 9
G2 Phase
Tubulin
Acetone
Cell Cycle Checkpoints
Heterografts
Caspase 3
Polymerization
Cell Division
Hepatitis
Liver Diseases
Fibrosis
Growth
Neoplasms
A549 Cells
In Vitro Techniques

Keywords

  • Apoptosis
  • Bupleurum scorzonerifolium
  • Caspases
  • Cell cycle
  • Lung cancer
  • Tubulin

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Cheng, Y. L., Lee, S. C., Lin, S. Z., Chang, W. L., Chen, Y. L., Tsai, N. M., ... Harn, H. J. (2005). Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo. Cancer Letters, 222(2), 183-193. https://doi.org/10.1016/j.canlet.2004.10.015

Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo. / Cheng, Yeung Leung; Lee, Shih Chun; Lin, Shinn Zong; Chang, Wen Liang; Chen, Yi Lin; Tsai, Nu Man; Liu, Yao Chi; Tzao, Ching; Yu, Dah Shyong; Harn, Horng Jyh.

In: Cancer Letters, Vol. 222, No. 2, 26.05.2005, p. 183-193.

Research output: Contribution to journalArticle

Cheng, YL, Lee, SC, Lin, SZ, Chang, WL, Chen, YL, Tsai, NM, Liu, YC, Tzao, C, Yu, DS & Harn, HJ 2005, 'Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo', Cancer Letters, vol. 222, no. 2, pp. 183-193. https://doi.org/10.1016/j.canlet.2004.10.015
Cheng, Yeung Leung ; Lee, Shih Chun ; Lin, Shinn Zong ; Chang, Wen Liang ; Chen, Yi Lin ; Tsai, Nu Man ; Liu, Yao Chi ; Tzao, Ching ; Yu, Dah Shyong ; Harn, Horng Jyh. / Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo. In: Cancer Letters. 2005 ; Vol. 222, No. 2. pp. 183-193.
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