Abstract

Diabetic retinopathy (DR), a major microvascular complication of diabetes, leads to retinal vascular leakage, neuronal dysfunction, and apoptosis within the retina. In this study, we combined STZ with whole-body hypoxia (10% O 2) for quicker induction of early-stage retinopathy in C57BL/6 mice. We also compared the effects of a high glucose condition combined with hypoxia (1% O 2) to a low glucose condition by using retinal pigment epithelial (RPE) cells, which are a crucial component of the outer blood-retinal barrier and the damage is related to retinopathy. In the retina of DM/hypoxic C57BL/6 mice, abnormal a-wave and b-wave activity, yellowish-white spots, hyperfluorescence, and reduced retinal thickness were found using electroretinography (ERG), fundus photography (FP), fundus fluorescein angiography (FFA), and optical coherence tomography (OCT). Shikonin dose-dependently (0.5-50 mg/kg, per os) prevented DM/hypoxia-induced lesions. In eye tissue, administration of shikonin also attenuated DM/hypoxia-induced pre-apoptotic protein BAX expression as well as the production of inflammatory proteins cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). We also demonstrated that shikonin administration rescues high glucose/hypoxia (1% O 2)-induced inflammation, decreased junction protein expression, and permeability in RPE cells. These results indicate that shikonin treatment may prevent the loss of vision associated with DR.

Original languageEnglish
Article number44985
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - Mar 21 2017

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Diabetic Retinopathy
Anti-Inflammatory Agents
Retinal Pigments
Inbred C57BL Mouse
Glucose
Retina
Epithelial Cells
Blood-Retinal Barrier
Electroretinography
Retinal Vessels
Proteins
Fluorescein Angiography
Photography
Optical Coherence Tomography
Dental Caries
Nitric Oxide Synthase Type II
Diabetes Complications
Cyclooxygenase 2
Permeability
shikonin

ASJC Scopus subject areas

  • General

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Anti-inflammatory properties of shikonin contribute to improved early-stage diabetic retinopathy. / Liao, Po Lin; Lin, Cheng Hui; Li, Ching Hao; Tsai, Chi Hao; Ho, Jau Der; Chiou, George C Y; Kang, Jaw Jou; Cheng, Yu Wen.

In: Scientific Reports, Vol. 7, 44985, 21.03.2017.

Research output: Contribution to journalArticle

Liao, Po Lin ; Lin, Cheng Hui ; Li, Ching Hao ; Tsai, Chi Hao ; Ho, Jau Der ; Chiou, George C Y ; Kang, Jaw Jou ; Cheng, Yu Wen. / Anti-inflammatory properties of shikonin contribute to improved early-stage diabetic retinopathy. In: Scientific Reports. 2017 ; Vol. 7.
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abstract = "Diabetic retinopathy (DR), a major microvascular complication of diabetes, leads to retinal vascular leakage, neuronal dysfunction, and apoptosis within the retina. In this study, we combined STZ with whole-body hypoxia (10{\%} O 2) for quicker induction of early-stage retinopathy in C57BL/6 mice. We also compared the effects of a high glucose condition combined with hypoxia (1{\%} O 2) to a low glucose condition by using retinal pigment epithelial (RPE) cells, which are a crucial component of the outer blood-retinal barrier and the damage is related to retinopathy. In the retina of DM/hypoxic C57BL/6 mice, abnormal a-wave and b-wave activity, yellowish-white spots, hyperfluorescence, and reduced retinal thickness were found using electroretinography (ERG), fundus photography (FP), fundus fluorescein angiography (FFA), and optical coherence tomography (OCT). Shikonin dose-dependently (0.5-50 mg/kg, per os) prevented DM/hypoxia-induced lesions. In eye tissue, administration of shikonin also attenuated DM/hypoxia-induced pre-apoptotic protein BAX expression as well as the production of inflammatory proteins cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). We also demonstrated that shikonin administration rescues high glucose/hypoxia (1{\%} O 2)-induced inflammation, decreased junction protein expression, and permeability in RPE cells. These results indicate that shikonin treatment may prevent the loss of vision associated with DR.",
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